##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631428.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711733
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83212665423691	34.0	33.0	34.0	31.0	34.0
2	33.00946141319849	34.0	33.0	34.0	31.0	34.0
3	33.119199194079805	34.0	33.0	34.0	31.0	34.0
4	36.44219531762613	37.0	37.0	37.0	35.0	37.0
5	36.43228429762284	37.0	37.0	37.0	35.0	37.0
6	36.483127802139286	37.0	37.0	37.0	35.0	37.0
7	36.471939617806115	37.0	37.0	37.0	35.0	37.0
8	36.453738972339345	37.0	37.0	37.0	35.0	37.0
9	38.30969338220934	39.0	39.0	39.0	37.0	39.0
10	38.26393745969345	39.0	39.0	39.0	37.0	39.0
11	38.330116771317336	39.0	39.0	39.0	37.0	39.0
12	38.10307798008523	39.0	39.0	39.0	37.0	39.0
13	38.22960717010452	39.0	39.0	39.0	37.0	39.0
14	39.749934315255864	41.0	40.0	41.0	38.0	41.0
15	39.753134953697526	41.0	40.0	41.0	38.0	41.0
16	39.74392363428421	41.0	40.0	41.0	38.0	41.0
17	39.72841079449737	41.0	40.0	41.0	38.0	41.0
18	39.72245069429126	41.0	40.0	41.0	38.0	41.0
19	39.72846137526292	41.0	40.0	41.0	38.0	41.0
20	39.70560870438774	41.0	40.0	41.0	38.0	41.0
21	39.669093606731735	41.0	40.0	41.0	37.0	41.0
22	39.623271648216395	41.0	40.0	41.0	37.0	41.0
23	39.56331377075392	41.0	39.0	41.0	37.0	41.0
24	39.528796613336745	41.0	39.0	41.0	37.0	41.0
25	39.50173449875164	41.0	39.0	41.0	37.0	41.0
26	39.449754332031816	41.0	39.0	41.0	37.0	41.0
27	39.38343873334523	41.0	39.0	41.0	36.0	41.0
28	39.29215450175838	41.0	39.0	41.0	36.0	41.0
29	39.226105857112145	41.0	39.0	41.0	36.0	41.0
30	39.162979656697104	41.0	39.0	41.0	36.0	41.0
31	39.123574430299	40.0	39.0	41.0	35.0	41.0
32	39.05596761706988	40.0	39.0	41.0	35.0	41.0
33	38.97451150923169	40.0	39.0	41.0	35.0	41.0
34	38.93062707504078	40.0	39.0	41.0	35.0	41.0
35	38.828556495202555	40.0	38.0	41.0	35.0	41.0
36	38.78897845118886	40.0	38.0	41.0	35.0	41.0
37	38.738559263094444	40.0	38.0	41.0	35.0	41.0
38	38.66472118055507	40.0	38.0	41.0	35.0	41.0
39	38.59141981613892	40.0	38.0	41.0	35.0	41.0
40	38.51252646708808	40.0	38.0	41.0	35.0	41.0
41	38.43939651526626	40.0	38.0	41.0	35.0	41.0
42	38.34735778726011	40.0	37.0	41.0	34.0	41.0
43	37.398197076712755	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	8.0
18	11.0
19	28.0
20	46.0
21	89.0
22	160.0
23	271.0
24	433.0
25	662.0
26	1002.0
27	1396.0
28	1970.0
29	2739.0
30	3851.0
31	4989.0
32	6801.0
33	9436.0
34	14216.0
35	21058.0
36	34782.0
37	64823.0
38	158283.0
39	384676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76833306872099	16.41865699637364	11.698347554490237	27.114662380415126
2	21.445261074026355	19.070634634055185	31.569844309593627	27.914259982324836
3	20.268274760338496	20.144913893271774	28.76303332850943	30.8237780178803
4	15.006470122925311	15.078126207440148	33.029801906051844	36.88560176358269
5	16.73043121507644	33.47870620021834	32.68585270038062	17.1050098843246
6	35.95154362661279	33.33272449078517	15.565387582141055	15.150344300460988
7	32.31492708642145	28.444515007734644	19.271131168570236	19.96942673727367
8	29.382085697867037	30.812959354139824	18.70308107113201	21.101873876861127
9	28.283218566513003	13.833136864526443	17.847001614369436	40.036642954591116
10	19.273800708973727	23.5638926395151	29.796847975294106	27.365458676217063
11	38.88480652154671	20.47144083525704	19.210715254175373	21.433037389020885
12	25.568155474033098	23.563330631009098	25.39899091372748	25.46952298123032
13	32.2026658873482	17.143366964859013	23.209265272229896	27.444701875562888
14	24.98479064480641	18.5308254640434	21.965118942075186	34.51926494907501
15	28.300359825945964	24.55794518450037	19.859835078603915	27.281859910949752
16	28.25891169862856	23.26532562070327	21.33159485368811	27.14416782698006
17	26.609838239901762	23.89351062828336	22.260313909851025	27.23633722196385
18	27.201071188212435	21.670204978552352	23.70158472348479	27.427139109750424
19	27.240692787885344	22.923483946929537	22.925731980953532	26.910091284231584
20	27.798486230089093	21.879272142783883	22.877820755817137	27.444420871309887
21	29.62880743200048	22.287149816012466	22.28167023307898	25.802372518908072
22	29.27108901793229	22.02736138411455	21.366158376807032	27.335391221146132
23	27.578038393611088	21.946994167756728	22.690390919066562	27.784576519565622
24	26.752869404678442	22.70387912321053	22.37819519398426	28.16505627812677
25	27.352110974199594	21.79117730946858	22.71244975292701	28.144261963404816
26	27.610915891212013	22.54356619687439	22.63278504720169	27.21273286471191
27	27.81942104693755	21.845130126044456	22.477811201672537	27.85763762534546
28	26.590589448571304	22.234321016448582	23.637515753800933	27.537573781179177
29	26.499544070599505	22.48230726972053	23.841665343604976	27.17648331607499
30	25.753618281012685	23.179478821411962	24.32808370554688	26.738819192028473
31	27.374591314439545	22.767105080135387	22.448727261487104	27.409576343937964
32	25.7010704857018	22.029328413885544	23.805415794968056	28.4641853054446
33	25.735493506694223	21.67427954022084	24.425451679211164	28.164775273873772
34	27.14416782698006	21.01574607331682	24.49078516803352	27.3493009316696
35	26.557992955223376	21.23773943318632	25.162525834828507	27.04174177676179
36	25.120094192625604	21.606417013121494	25.364146386355557	27.90934240789734
37	25.750527234229693	20.121309536019826	25.990083359911655	28.138079869838826
38	24.386392088044254	19.432427609791876	27.562161653316625	28.61901864884725
39	24.60093883520927	18.872948142070133	28.566330351409867	27.95978267131073
40	23.886485521958374	19.67507478225683	28.89622934443113	27.542210351353667
41	22.706267659361025	19.151563858919005	30.87716882595018	27.26499965576979
42	22.586700349709794	19.445634809682844	30.720087448523532	27.247577392083826
43	20.562767217481838	18.915801290652535	31.337453792363146	29.183977699502485
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	22.0
4	41.0
5	41.0
6	58.0
7	75.0
8	72.0
9	69.0
10	99.5
11	130.0
12	130.0
13	224.5
14	319.0
15	556.5
16	794.0
17	741.5
18	689.0
19	689.0
20	786.5
21	884.0
22	841.5
23	799.0
24	1027.0
25	1255.0
26	1255.0
27	1697.0
28	2139.0
29	3070.0
30	4001.0
31	5278.0
32	6555.0
33	6555.0
34	8912.0
35	11269.0
36	14788.5
37	18308.0
38	22307.0
39	26306.0
40	26306.0
41	29456.5
42	32607.0
43	36470.5
44	40334.0
45	44041.0
46	47748.0
47	47748.0
48	52113.5
49	56479.0
50	58648.0
51	60817.0
52	62405.5
53	63994.0
54	63994.0
55	61586.0
56	59178.0
57	57274.5
58	55371.0
59	53796.0
60	52221.0
61	52221.0
62	48823.5
63	45426.0
64	42317.0
65	39208.0
66	35047.5
67	30887.0
68	30887.0
69	25673.0
70	20459.0
71	17365.0
72	14271.0
73	10769.5
74	7268.0
75	7268.0
76	5638.0
77	4008.0
78	3407.0
79	2806.0
80	2456.0
81	2106.0
82	2106.0
83	1663.5
84	1221.0
85	995.0
86	769.0
87	640.0
88	511.0
89	511.0
90	405.5
91	300.0
92	180.5
93	61.0
94	42.0
95	23.0
96	23.0
97	19.0
98	15.0
99	11.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	711733.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0102272430928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04922269191502	44.754396262979974
2	6.364886229146648	6.6207835830869834
3	2.1835833606629453	3.407060003769481
4	1.172301875221182	2.4388674771103
5	0.7577576177564581	1.970557294734902
6	0.5103807728583726	1.5927011986121575
7	0.3711392674542405	1.351212634739103
8	0.3018936751475383	1.2561246918140723
9	0.23020379508260141	1.0775656525021622
>10	1.7564699124212366	17.789061007936553
>50	0.19556371318478824	7.002123097107976
>100	0.1041559344357242	9.825257941463311
>500	0.0021699153007427707	0.7658811630678036
>1k	2.7123941259284633E-4	0.14840799107521263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1052	0.14780823707766816	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	811	0.11394722459124419	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	810	0.11380672246474449	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	796	0.11183969269374892	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	732	0.10284755659776909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.8100425299936913E-4	0.0
18	0.0	0.0	0.0	2.8100425299936913E-4	1.4050212649968457E-4
19	0.0	0.0	0.0	5.620085059987383E-4	1.4050212649968457E-4
20	0.0	0.0	0.0	7.025106324984228E-4	1.4050212649968457E-4
21	0.0	0.0	0.0	0.0014050212649968457	1.4050212649968457E-4
22	0.0	0.0	0.0	0.0015455233914965303	1.4050212649968457E-4
23	0.0	1.4050212649968457E-4	0.0	0.0032315489094927453	1.4050212649968457E-4
24	0.0	1.4050212649968457E-4	0.0	0.007165608451483913	1.4050212649968457E-4
25	0.0	1.4050212649968457E-4	0.0	0.010537659487476344	1.4050212649968457E-4
26	0.0	1.4050212649968457E-4	0.0	0.017562765812460572	1.4050212649968457E-4
27	0.0	1.4050212649968457E-4	0.0	0.06252344629235963	1.4050212649968457E-4
28	0.0	1.4050212649968457E-4	0.0	0.24264717246495526	2.8100425299936913E-4
29	0.0	4.215063794990537E-4	0.0	0.5299740211568102	2.8100425299936913E-4
30	0.0	4.215063794990537E-4	0.0	0.9218344519644305	2.8100425299936913E-4
31	0.0	4.215063794990537E-4	0.0	1.9629552093270932	2.8100425299936913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGTA	85	1.9736035E-9	23.941177	5
CGCTTAT	115	5.456968E-12	22.52174	26
CCGCTTA	120	1.0913936E-11	21.583332	25
CAATATA	60	3.72372E-5	21.583332	2
CTTATAC	1430	0.0	20.958042	37
CGTAACG	45	0.0038243982	20.555555	26
GGTATCA	695	0.0	20.4964	1
GGGTATC	55	5.1404507E-4	20.181818	1
TAGCGAA	55	5.1404507E-4	20.181818	10
CGTCCTA	55	5.1404507E-4	20.181818	33
CCTAAAC	75	9.257643E-6	19.733334	3
GCAGTCG	335	0.0	18.77612	9
GATCCGT	60	9.232105E-4	18.5	29
TCGGGTA	60	9.232105E-4	18.5	25
GTCTAGG	120	5.164111E-9	18.5	1
GTAGCGA	60	9.232105E-4	18.5	9
GCTTATT	140	9.458745E-11	18.5	27
ATAAGGT	50	0.0070323134	18.5	4
TAGATAA	180	0.0	18.5	4
TATACAC	340	0.0	18.5	37
>>END_MODULE