FastQCFastQC Report
Fri 10 Feb 2017
ERR1631427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048063
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT138320.45379639462832627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110480.3624596998159159No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101300.3323422120868237No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA49060.16095467843020306No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT44970.1475363206075465No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC43670.14327131689863365No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC40740.13365865469316088No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT38730.12706430280476486No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC37790.1239803770460125No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC36820.12079802812474676No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC35090.11512229241980891No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.10885601773979081No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG31740.1041317059391489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA500.00703748418.52
AAGACGG12300.018.3495945
GCAGTCG21850.018.1189929
ACGGACC12150.017.967088
CAGTCGG21600.017.81481610
AGTCGGT21400.017.80841311
CAAGACG13050.017.7203064
CGCCTTA15050.017.70099625
CTTATAC27250.017.51559637
GGTATCA65950.017.3919621
CGGACCA12850.017.1322969
AGACGGA13500.016.9925926
GCCTTAT15300.016.92810426
CTCTATG35100.016.5498581
GTTCAAA12900.016.4922471
CGGTGAT23500.016.29574614
TCGGTGA23750.016.2813
CGCAAGA13650.016.2637372
TCGTTTA10150.016.22167630
GCGAAAG13700.016.2043818
Produced by FastQC (version 0.11.5)