##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3048063
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87230578895515	34.0	33.0	34.0	31.0	34.0
2	33.03890273921503	34.0	33.0	34.0	31.0	34.0
3	33.14756092639818	34.0	33.0	34.0	31.0	34.0
4	36.46865304293251	37.0	37.0	37.0	35.0	37.0
5	36.45155628344952	37.0	37.0	37.0	35.0	37.0
6	36.498074350825426	37.0	37.0	37.0	35.0	37.0
7	36.48408809135507	37.0	37.0	37.0	35.0	37.0
8	36.472486297035196	37.0	37.0	37.0	35.0	37.0
9	38.325070052685916	39.0	39.0	39.0	37.0	39.0
10	38.28706624502184	39.0	39.0	39.0	37.0	39.0
11	38.3448527802739	39.0	39.0	39.0	37.0	39.0
12	38.12082427430142	39.0	39.0	39.0	37.0	39.0
13	38.24787414170901	39.0	39.0	39.0	37.0	39.0
14	39.77071864984418	41.0	40.0	41.0	38.0	41.0
15	39.77567425607673	41.0	40.0	41.0	38.0	41.0
16	39.751620947467295	41.0	40.0	41.0	38.0	41.0
17	39.74468277066452	41.0	40.0	41.0	38.0	41.0
18	39.735810906795564	41.0	40.0	41.0	38.0	41.0
19	39.74262572656799	41.0	40.0	41.0	38.0	41.0
20	39.73234936416996	41.0	40.0	41.0	38.0	41.0
21	39.69348533806552	41.0	40.0	41.0	37.0	41.0
22	39.638704646196615	41.0	40.0	41.0	37.0	41.0
23	39.56761622053087	41.0	40.0	41.0	37.0	41.0
24	39.530345993504724	41.0	39.0	41.0	37.0	41.0
25	39.51034739111363	41.0	39.0	41.0	37.0	41.0
26	39.43342509652852	41.0	39.0	41.0	36.0	41.0
27	39.34878281715306	41.0	39.0	41.0	36.0	41.0
28	39.22956054386015	41.0	39.0	41.0	36.0	41.0
29	39.140815330916716	41.0	39.0	41.0	35.0	41.0
30	39.06343044746779	41.0	39.0	41.0	35.0	41.0
31	38.989703624892265	40.0	39.0	41.0	35.0	41.0
32	38.89921468158631	40.0	38.0	41.0	35.0	41.0
33	38.77627988660339	40.0	38.0	41.0	35.0	41.0
34	38.72709651998663	40.0	38.0	41.0	35.0	41.0
35	38.593507089584435	40.0	38.0	41.0	35.0	41.0
36	38.52419192122998	40.0	38.0	41.0	35.0	41.0
37	38.43195793525265	40.0	38.0	41.0	35.0	41.0
38	38.31919583026991	40.0	37.0	41.0	35.0	41.0
39	38.209121989932626	40.0	37.0	41.0	34.0	41.0
40	38.084329621795874	40.0	37.0	41.0	34.0	41.0
41	37.96615621133815	40.0	37.0	41.0	34.0	41.0
42	37.81182081866418	40.0	36.0	41.0	34.0	41.0
43	36.848121249462366	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	6.0
12	2.0
13	1.0
14	15.0
15	9.0
16	23.0
17	58.0
18	109.0
19	156.0
20	284.0
21	507.0
22	920.0
23	1513.0
24	2248.0
25	3358.0
26	4980.0
27	6854.0
28	9976.0
29	13584.0
30	18314.0
31	23880.0
32	31479.0
33	43278.0
34	65621.0
35	98508.0
36	162007.0
37	317005.0
38	652047.0
39	1591316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7318182071696	16.549690738019525	11.594117313192017	27.124373741618857
2	20.82233864588757	19.610126168652027	30.733682341867606	28.8338528435928
3	20.96698788706139	20.593176715835597	28.25528868661835	30.18454671048466
4	14.897953224720093	14.790967247068057	32.212884051281094	38.098195476930755
5	17.019694146741717	34.121670057344616	31.37717297838004	17.48146281753363
6	36.30892799787931	32.40963195314532	15.413231288198439	15.868208760776925
7	31.856657818424356	28.470441719872586	19.157871736903076	20.51502872479998
8	31.115006481165253	28.438552615218253	18.612148108487258	21.834292795129233
9	28.52710065375945	12.963052272869687	17.73733023234756	40.7725168410233
10	19.799328294723566	23.888088927295794	28.660726500731776	27.651856277248864
11	39.26762668619382	20.388390922366106	18.522189337950035	21.821793053490033
12	25.226840783802697	23.95203117520865	24.89364557097409	25.927482470014564
13	32.83652601668666	16.634105003735158	22.957858810661065	27.57151016891711
14	25.083241389695687	19.13388929297065	21.572421567401985	34.210447749931674
15	28.712497084213812	25.02435153079185	19.213874516373185	27.049276868621153
16	28.121630031925193	23.36788970569178	21.47698390748485	27.03349635489818
17	26.912206210960864	23.71437204545969	22.200131690191444	27.173290053388005
18	27.149077955409712	21.061539738515904	24.255961901049947	27.53342040502444
19	27.38227523512473	22.938830332575147	23.34840191951413	26.33049251278599
20	27.875145625270868	21.239029508248354	23.06992998504296	27.81589488143782
21	29.538431456305204	21.95791228724603	22.673383063276578	25.830273193172186
22	29.17846514327296	21.531477531796423	22.13202286173219	27.158034463198433
23	27.03598974168185	21.53905611530995	22.96550300961627	28.459451133391926
24	26.502076892767633	22.710587018706637	23.590424476134515	27.19691161239121
25	26.9980968241142	21.677307850920403	22.80317040691088	28.521424918054517
26	27.297729738525746	23.02271967475738	22.90999234595873	26.769558240758144
27	28.00480173802182	21.405397460616793	22.624302712903244	27.965498088458148
28	26.34932414454688	22.301540355301057	23.790321919199176	27.558813580952883
29	25.893428055784934	22.777153884286513	24.40297329812409	26.926444761804465
30	25.398129894296805	23.57431588520316	24.379712624050093	26.647841596449943
31	26.784288907414318	23.490164081254225	22.600189038087468	27.12535797324399
32	25.715511785681596	22.636900877704953	23.452336779128252	28.195250557485195
33	25.279169098538972	22.363382909080293	24.348446866091678	28.009001126289057
34	27.288248307203624	22.096918600435753	23.70498903730008	26.909844055060546
35	26.727367511760747	22.33011588015077	24.584957725611314	26.357558882477168
36	24.69135316428827	23.70016630233693	24.4874531792814	27.121027354093403
37	25.87633523322845	22.064274918202152	25.150365986529806	26.9090238620396
38	25.134519857365152	21.508380896326617	25.925218737276758	27.431880509031476
39	25.611478502904962	20.92400977276388	25.98768463775191	27.47682708657925
40	24.75821529935569	21.38138220896353	26.614180874870367	27.246221616810413
41	23.27770128110869	21.468191438300323	28.215886613892167	27.03822066669882
42	24.43381255571161	21.316062036775488	28.07845507130266	26.17167033621024
43	22.95067391979759	20.497082901501706	28.61046507240828	27.94177810629242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	125.0
2	139.0
3	544.5
4	950.0
5	950.0
6	1248.5
7	1547.0
8	1532.5
9	1518.0
10	2392.5
11	3267.0
12	3267.0
13	6298.0
14	9329.0
15	16885.5
16	24442.0
17	21881.5
18	19321.0
19	19321.0
20	21330.5
21	23340.0
22	17374.5
23	11409.0
24	12312.5
25	13216.0
26	13216.0
27	15005.0
28	16794.0
29	20183.0
30	23572.0
31	28591.0
32	33610.0
33	33610.0
34	41266.5
35	48923.0
36	61593.5
37	74264.0
38	86716.0
39	99168.0
40	99168.0
41	108693.5
42	118219.0
43	127012.0
44	135805.0
45	151278.0
46	166751.0
47	166751.0
48	188853.0
49	210955.0
50	218864.0
51	226773.0
52	232588.0
53	238403.0
54	238403.0
55	233188.5
56	227974.0
57	228179.5
58	228385.0
59	229467.5
60	230550.0
61	230550.0
62	218675.0
63	206800.0
64	194130.0
65	181460.0
66	163600.0
67	145740.0
68	145740.0
69	129424.0
70	113108.0
71	98634.5
72	84161.0
73	63762.0
74	43363.0
75	43363.0
76	34959.0
77	26555.0
78	22985.0
79	19415.0
80	17090.5
81	14766.0
82	14766.0
83	11952.5
84	9139.0
85	8030.0
86	6921.0
87	5922.0
88	4923.0
89	4923.0
90	3650.0
91	2377.0
92	1396.0
93	415.0
94	275.5
95	136.0
96	136.0
97	87.0
98	38.0
99	24.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3048063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.69663016601404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.72449340215869	36.17450360794866
2	8.443856403450397	7.210484280661021
3	2.3781516330227093	3.0461718226161882
4	1.1001354782246575	1.8788831058507678
5	0.6745321589440832	1.4400125062759261
6	0.4289982263847849	1.0990067168296276
7	0.3013366337642638	0.900624116511307
8	0.22872328283198468	0.7812570733947102
9	0.18931346365711013	0.7274742248893293
>10	1.1870494490573271	9.974566936748984
>50	0.15175731009596838	4.571715777013938
>100	0.15301415492398202	13.743024596234141
>500	0.024910587475915104	7.409755665875046
>1k	0.013496448322863744	9.889684556608138
>5k	0.0	0.0
>10k+	2.3136768553480707E-4	1.1528350125421225
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13832	0.45379639462832627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11048	0.3624596998159159	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10130	0.3323422120868237	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	4906	0.16095467843020306	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4497	0.1475363206075465	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	4367	0.14327131689863365	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	4074	0.13365865469316088	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3873	0.12706430280476486	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	3779	0.1239803770460125	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3682	0.12079802812474676	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	3509	0.11512229241980891	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3318	0.10885601773979081	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	3174	0.1041317059391489	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.280772083779108E-5
2	3.280772083779108E-5	3.280772083779108E-5	0.0	0.0	3.280772083779108E-5
3	3.280772083779108E-5	3.280772083779108E-5	0.0	0.0	3.280772083779108E-5
4	3.280772083779108E-5	3.280772083779108E-5	0.0	0.0	3.280772083779108E-5
5	3.280772083779108E-5	6.561544167558216E-5	0.0	0.0	3.280772083779108E-5
6	3.280772083779108E-5	6.561544167558216E-5	0.0	0.0	3.280772083779108E-5
7	3.280772083779108E-5	6.561544167558216E-5	0.0	3.280772083779108E-5	3.280772083779108E-5
8	3.280772083779108E-5	6.561544167558216E-5	0.0	3.280772083779108E-5	6.561544167558216E-5
9	3.280772083779108E-5	6.561544167558216E-5	0.0	3.280772083779108E-5	6.561544167558216E-5
10	3.280772083779108E-5	6.561544167558216E-5	0.0	3.280772083779108E-5	9.842316251337324E-5
11	3.280772083779108E-5	6.561544167558216E-5	0.0	3.280772083779108E-5	9.842316251337324E-5
12	3.280772083779108E-5	6.561544167558216E-5	0.0	6.561544167558216E-5	9.842316251337324E-5
13	3.280772083779108E-5	6.561544167558216E-5	0.0	1.640386041889554E-4	9.842316251337324E-5
14	3.280772083779108E-5	6.561544167558216E-5	0.0	3.93692650053493E-4	9.842316251337324E-5
15	3.280772083779108E-5	6.561544167558216E-5	0.0	5.249235334046573E-4	9.842316251337324E-5
16	3.280772083779108E-5	9.842316251337324E-5	0.0	0.0014435397168628076	1.3123088335116433E-4
17	9.842316251337324E-5	9.842316251337324E-5	0.0	0.002001270971105256	1.3123088335116433E-4
18	9.842316251337324E-5	1.3123088335116433E-4	0.0	0.002493386783672122	1.3123088335116433E-4
19	9.842316251337324E-5	1.3123088335116433E-4	0.0	0.0035104261296436457	1.3123088335116433E-4
20	9.842316251337324E-5	1.3123088335116433E-4	0.0	0.0041993882672372584	1.640386041889554E-4
21	9.842316251337324E-5	1.3123088335116433E-4	0.0	0.005610120263262275	2.2965404586453757E-4
22	9.842316251337324E-5	1.3123088335116433E-4	0.0	0.008201930209447771	2.2965404586453757E-4
23	9.842316251337324E-5	2.2965404586453757E-4	0.0	0.011613933176578044	2.2965404586453757E-4
24	9.842316251337324E-5	2.2965404586453757E-4	0.0	0.016863168510624617	2.2965404586453757E-4
25	9.842316251337324E-5	2.2965404586453757E-4	0.0	0.01968463250267465	2.2965404586453757E-4
26	9.842316251337324E-5	2.2965404586453757E-4	0.0	0.024769829232532268	2.2965404586453757E-4
27	9.842316251337324E-5	2.2965404586453757E-4	0.0	0.04474973122274704	2.2965404586453757E-4
28	9.842316251337324E-5	2.6246176670232865E-4	0.0	0.12306176086255435	2.6246176670232865E-4
29	9.842316251337324E-5	2.9526948754011973E-4	0.0	0.24458155884573252	2.6246176670232865E-4
30	9.842316251337324E-5	2.9526948754011973E-4	0.0	0.397924846041568	2.6246176670232865E-4
31	9.842316251337324E-5	2.9526948754011973E-4	0.0	0.8132049764063276	2.6246176670232865E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	50	0.007037484	18.5	2
AAGACGG	1230	0.0	18.349594	5
GCAGTCG	2185	0.0	18.118992	9
ACGGACC	1215	0.0	17.96708	8
CAGTCGG	2160	0.0	17.814816	10
AGTCGGT	2140	0.0	17.808413	11
CAAGACG	1305	0.0	17.720306	4
CGCCTTA	1505	0.0	17.700996	25
CTTATAC	2725	0.0	17.515596	37
GGTATCA	6595	0.0	17.391962	1
CGGACCA	1285	0.0	17.132296	9
AGACGGA	1350	0.0	16.992592	6
GCCTTAT	1530	0.0	16.928104	26
CTCTATG	3510	0.0	16.549858	1
GTTCAAA	1290	0.0	16.492247	1
CGGTGAT	2350	0.0	16.295746	14
TCGGTGA	2375	0.0	16.28	13
CGCAAGA	1365	0.0	16.263737	2
TCGTTTA	1015	0.0	16.221676	30
GCGAAAG	1370	0.0	16.20438	18
>>END_MODULE