FastQCFastQC Report
Fri 10 Feb 2017
ERR1631426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599253
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.507965750692946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24590.41034421187712034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21920.3657887403150255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.14501387560846588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.021.97381
GCTATAC450.003823703420.5555553
AGCTATA450.003823703420.5555552
GCAGTCG2400.020.0416689
CGCTTAT656.895087E-519.92307726
TTTCGGG851.2432738E-619.58823422
GGCAGTC2500.019.248
CAGTCGG2550.018.86274510
AGTCGGT2650.018.84905611
TTTAGGA1501.2732926E-1118.52
CCGCTTA701.21796176E-418.525
ACTAGTC701.21796176E-418.53
ACGCTTT500.007031047718.528
CAATACG500.007031047718.54
TCGGTGA2900.017.86206813
GTCGGTG2850.017.52631812
GCTCGTC953.6005604E-617.52631612
TAGCTCG953.6005604E-617.52631610
TTAGGAG1705.456968E-1217.4117643
GTCTAAA752.065186E-417.2666661
Produced by FastQC (version 0.11.5)