##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631426.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599253
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78637737316292	34.0	31.0	34.0	31.0	34.0
2	32.957450359030325	34.0	33.0	34.0	31.0	34.0
3	33.06831505224004	34.0	33.0	34.0	31.0	34.0
4	36.411011709578425	37.0	37.0	37.0	35.0	37.0
5	36.38551830362134	37.0	37.0	37.0	35.0	37.0
6	36.435230194926014	37.0	37.0	37.0	35.0	37.0
7	36.43326775168418	37.0	37.0	37.0	35.0	37.0
8	36.421329555296346	37.0	37.0	37.0	35.0	37.0
9	38.270231438140485	39.0	39.0	39.0	37.0	39.0
10	38.22010903574951	39.0	39.0	39.0	37.0	39.0
11	38.2906251616596	39.0	39.0	39.0	37.0	39.0
12	38.0709299744849	39.0	39.0	39.0	37.0	39.0
13	38.203952253889426	39.0	39.0	39.0	37.0	39.0
14	39.69898023038683	41.0	40.0	41.0	37.0	41.0
15	39.692230159882385	41.0	40.0	41.0	37.0	41.0
16	39.66464247988746	41.0	40.0	41.0	37.0	41.0
17	39.665995831476856	41.0	40.0	41.0	37.0	41.0
18	39.66771296931346	41.0	40.0	41.0	37.0	41.0
19	39.68591896911655	41.0	40.0	41.0	37.0	41.0
20	39.656555745236155	41.0	40.0	41.0	37.0	41.0
21	39.63188336145167	41.0	40.0	41.0	37.0	41.0
22	39.576944963145785	41.0	40.0	41.0	37.0	41.0
23	39.516185984884515	41.0	39.0	41.0	37.0	41.0
24	39.49338426340794	41.0	39.0	41.0	37.0	41.0
25	39.475143219975536	41.0	39.0	41.0	37.0	41.0
26	39.3961632232129	41.0	39.0	41.0	36.0	41.0
27	39.32168549844557	41.0	39.0	41.0	36.0	41.0
28	39.25163328343788	41.0	39.0	41.0	36.0	41.0
29	39.167748847314904	41.0	39.0	41.0	35.0	41.0
30	39.09023400800663	40.0	39.0	41.0	35.0	41.0
31	39.01055814489039	40.0	39.0	41.0	35.0	41.0
32	38.913357129626384	40.0	38.0	41.0	35.0	41.0
33	38.818198657328374	40.0	38.0	41.0	35.0	41.0
34	38.77761312834479	40.0	38.0	41.0	35.0	41.0
35	38.67315474432335	40.0	38.0	41.0	35.0	41.0
36	38.5991309179929	40.0	38.0	41.0	35.0	41.0
37	38.53430020375367	40.0	38.0	41.0	35.0	41.0
38	38.439557248774726	40.0	38.0	41.0	35.0	41.0
39	38.34340253615752	40.0	38.0	41.0	35.0	41.0
40	38.241312100231454	40.0	38.0	41.0	34.0	41.0
41	38.141320944575995	40.0	38.0	41.0	34.0	41.0
42	38.017448389912104	40.0	37.0	41.0	34.0	41.0
43	37.113162554046454	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	2.0
15	3.0
16	2.0
17	5.0
18	12.0
19	27.0
20	62.0
21	83.0
22	163.0
23	287.0
24	408.0
25	662.0
26	997.0
27	1388.0
28	2053.0
29	2853.0
30	3857.0
31	5111.0
32	6654.0
33	9008.0
34	12869.0
35	18665.0
36	29842.0
37	58774.0
38	129544.0
39	315919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.431405433097545	18.606331549445727	12.26827400113141	25.693989016325325
2	19.340745895306323	20.710951801659732	33.7847286538407	26.163573649193246
3	19.939491333376722	22.435265238555335	29.57231753533149	28.052925892736457
4	14.206186702444542	15.895957133297623	34.90512354548079	34.99273261877705
5	15.157204052378544	35.9938122963089	33.05081493125608	15.798168720056472
6	33.456319784798744	35.07950731994667	16.17513804686835	15.28903484838624
7	29.734686351173877	30.338771770854716	20.526221812823632	19.400320065147774
8	28.65283945178414	31.410606204724882	19.71921709194614	20.21733725154484
9	27.588514367053644	13.684704123300175	19.764606935634866	38.96217457401131
10	18.183471755669142	25.86386718130739	31.552449466252153	24.400211596771314
11	36.06223081069265	21.693842166831036	20.59030159214889	21.653625430327423
12	22.83092450100375	26.33762367480847	27.439328630812028	23.392123193375753
13	31.368386975117357	19.07808554984289	23.98285865903049	25.570668816009263
14	23.5242877382341	20.789549655988374	24.59645592095492	31.089706684822605
15	26.831238224923364	26.562236651297532	21.957670633271757	24.64885449050735
16	25.292822897841145	25.575174425493074	23.87021842193531	25.261784254730475
17	23.40797626378174	26.06461711497481	25.064538683994908	25.46286793724854
18	23.7504025845511	24.383857903089346	26.670204404483584	25.195535107875973
19	24.39687410826479	25.196870103278577	26.70191054529556	23.704345243161068
20	25.45502483925821	24.281563880364388	26.31125751560693	23.952153764770472
21	26.389688495510242	24.769337825592864	25.062202442040338	23.778771236856553
22	26.08397454831265	24.36082923239433	25.25277303576286	24.302423183530163
23	24.9352110043671	24.536381127837494	25.55481574560328	24.97359212219213
24	24.498834382139094	25.11059602538494	25.335709625149978	25.05485996732599
25	24.57192538043197	24.525200541340634	25.594364984405587	25.308509093821808
26	24.422906518615676	24.853442535957267	25.73153576202372	24.99211518340334
27	24.514186829269107	24.495330019207245	25.529450832953692	25.461032318569956
28	24.064126504164353	24.76816970461558	26.535703617670663	24.6320001735494
29	23.771261887716875	24.95206532132505	26.824730122335644	24.451942668622433
30	23.886572115617277	25.551645131522076	26.436079585750928	24.12570316710972
31	24.529038653123138	25.640922949071594	25.40896749786818	24.42107089993709
32	23.286324807718945	25.1995400940838	26.43174085069245	25.08239424750481
33	23.294668528985255	24.501170624093664	27.180005773855115	25.02415507306597
34	24.228330938685332	23.953321885747755	27.121933473841597	24.696413701725316
35	24.056617155024675	24.490156912022133	27.031654409740124	24.421571523213068
36	23.169846458841256	24.976595861848	26.595110913086796	25.258446766223948
37	23.37042951808335	23.962833727991352	27.338870226765653	25.32786652715965
38	23.04802812835313	23.497921579032564	28.1875935539747	25.266456738639608
39	22.658710094067114	23.07539553410663	29.076032994411378	25.18986137741488
40	22.25737710115761	23.040018155937474	29.62004362097478	25.082561121930137
41	21.46906231591665	22.73830919494771	30.35729483206592	25.43533365706972
42	21.329054673067972	23.027168825187356	30.327758058783182	25.316018442961486
43	20.61850337002902	22.315115652320472	30.923833506048364	26.142547471602146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	79.0
2	77.0
3	211.5
4	346.0
5	346.0
6	443.5
7	541.0
8	532.0
9	523.0
10	779.5
11	1036.0
12	1036.0
13	1872.0
14	2708.0
15	4333.0
16	5958.0
17	5480.5
18	5003.0
19	5003.0
20	5661.0
21	6319.0
22	5427.0
23	4535.0
24	5169.0
25	5803.0
26	5803.0
27	6714.0
28	7625.0
29	9243.5
30	10862.0
31	12837.5
32	14813.0
33	14813.0
34	17319.5
35	19826.0
36	22440.0
37	25054.0
38	27278.5
39	29503.0
40	29503.0
41	31597.0
42	33691.0
43	35212.0
44	36733.0
45	38186.0
46	39639.0
47	39639.0
48	41519.0
49	43399.0
50	43928.5
51	44458.0
52	44094.5
53	43731.0
54	43731.0
55	42622.5
56	41514.0
57	39822.5
58	38131.0
59	36400.5
60	34670.0
61	34670.0
62	31525.0
63	28380.0
64	24973.0
65	21566.0
66	18935.0
67	16304.0
68	16304.0
69	14147.0
70	11990.0
71	10310.5
72	8631.0
73	6969.0
74	5307.0
75	5307.0
76	4318.0
77	3329.0
78	2840.0
79	2351.0
80	2011.5
81	1672.0
82	1672.0
83	1428.5
84	1185.0
85	1021.5
86	858.0
87	717.5
88	577.0
89	577.0
90	465.5
91	354.0
92	228.5
93	103.0
94	78.5
95	54.0
96	54.0
97	33.0
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	599253.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.21190514249164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.51366964633306	64.95560997592146
2	3.895535628001162	5.470259559848285
3	0.9946480436633802	2.0950840227557443
4	0.5325898792122487	1.4957660031640596
5	0.359340003852508	1.261497313219744
6	0.24778680810044482	1.0438550319545528
7	0.18313436886139856	0.9000749054378615
8	0.15363099909392464	0.8629380108263085
9	0.12366616576637195	0.781455339011174
>10	0.8733175504105027	11.913836126306157
>50	0.08575755347834428	4.051598560153432
>100	0.0359704924983206	3.7356497542521216
>500	2.3821518210711748E-4	0.1453449579778788
>1k	7.146455463213524E-4	1.2870304391712053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3044	0.507965750692946	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2459	0.41034421187712034	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2192	0.3657887403150255	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	869	0.14501387560846588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	3.3374885065239556E-4	0.0
13	0.0	0.0	0.0	3.3374885065239556E-4	0.0
14	0.0	0.0	0.0	3.3374885065239556E-4	0.0
15	0.0	0.0	0.0	5.006232759785933E-4	0.0
16	0.0	0.0	0.0	6.674977013047911E-4	0.0
17	0.0	0.0	0.0	0.0011681209772833846	0.0
18	0.0	0.0	0.0	0.0013349954026095822	0.0
19	0.0	0.0	0.0	0.0015018698279357801	0.0
20	0.0	0.0	0.0	0.0018356186785881757	0.0
21	0.0	0.0	0.0	0.002336241954566769	0.0
22	0.0	0.0	0.0	0.0033374885065239556	0.0
23	0.0	0.0	0.0	0.004839358334459735	0.0
24	0.0	0.0	0.0	0.008009972415657493	0.0
25	0.0	0.0	0.0	0.01118058649685525	1.6687442532619778E-4
26	0.0	0.0	0.0	0.01802243793522936	1.6687442532619778E-4
27	0.0	0.0	0.0	0.05373356495503569	1.6687442532619778E-4
28	0.0	0.0	0.0	0.164538183371631	1.6687442532619778E-4
29	0.0	0.0	0.0	0.31305642191194705	1.6687442532619778E-4
30	0.0	0.0	0.0	0.5209819558683895	1.6687442532619778E-4
31	0.0	0.0	0.0	1.0750050479513662	1.6687442532619778E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1145	0.0	21.9738	1
GCTATAC	45	0.0038237034	20.555555	3
AGCTATA	45	0.0038237034	20.555555	2
GCAGTCG	240	0.0	20.041668	9
CGCTTAT	65	6.895087E-5	19.923077	26
TTTCGGG	85	1.2432738E-6	19.588234	22
GGCAGTC	250	0.0	19.24	8
CAGTCGG	255	0.0	18.862745	10
AGTCGGT	265	0.0	18.849056	11
TTTAGGA	150	1.2732926E-11	18.5	2
CCGCTTA	70	1.21796176E-4	18.5	25
ACTAGTC	70	1.21796176E-4	18.5	3
ACGCTTT	50	0.0070310477	18.5	28
CAATACG	50	0.0070310477	18.5	4
TCGGTGA	290	0.0	17.862068	13
GTCGGTG	285	0.0	17.526318	12
GCTCGTC	95	3.6005604E-6	17.526316	12
TAGCTCG	95	3.6005604E-6	17.526316	10
TTAGGAG	170	5.456968E-12	17.411764	3
GTCTAAA	75	2.065186E-4	17.266666	1
>>END_MODULE