FastQCFastQC Report
Fri 10 Feb 2017
ERR1631425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285161
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102830.31301357832995097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82570.25134232386175287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74850.22784271455797753No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA54320.16534958256231583No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC45050.1371317874527306No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC41890.1275127763905635No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC38380.11682836853353609No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT36130.10997938913800571No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT33100.10075609688535814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA358.87292E-426.428572
GGTATCA53150.019.422391
CTTATAC65100.018.81259537
TCTAGAC5350.018.3271033
AGTCGGT16100.018.15527711
GCAGTCG16400.017.9359749
ATACACA14050.017.90747537
CTAGACA5950.017.7226894
GTTCTAG5450.016.9724771
CAGTCGG17550.016.9715110
GGCAGTC18700.016.323538
GTCTAGA4950.015.696971
TCGGTGA18400.015.58423913
AAGACGG10000.015.545
GTCGGTG18600.015.51612912
CTCTAGT5150.015.44660327
TCTAGTT5400.015.41666728
ACTGATC5650.015.389388
CTCTATG29950.015.3806331
CGTATCT1353.9783845E-715.07407527
Produced by FastQC (version 0.11.5)