##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631425.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3285161
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84819069750311	34.0	33.0	34.0	31.0	34.0
2	33.020501278323955	34.0	33.0	34.0	31.0	34.0
3	33.13207998025059	34.0	33.0	34.0	31.0	34.0
4	36.45744698661649	37.0	37.0	37.0	35.0	37.0
5	36.4391142473687	37.0	37.0	37.0	35.0	37.0
6	36.484723579757585	37.0	37.0	37.0	35.0	37.0
7	36.47434722377381	37.0	37.0	37.0	35.0	37.0
8	36.46257885077779	37.0	37.0	37.0	35.0	37.0
9	38.30945880582413	39.0	39.0	39.0	37.0	39.0
10	38.27512532871296	39.0	39.0	39.0	37.0	39.0
11	38.33093690080943	39.0	39.0	39.0	37.0	39.0
12	38.116610418789215	39.0	39.0	39.0	37.0	39.0
13	38.24082381350564	39.0	39.0	39.0	37.0	39.0
14	39.762199478199086	41.0	40.0	41.0	38.0	41.0
15	39.7583445681962	41.0	40.0	41.0	38.0	41.0
16	39.74410964942053	41.0	40.0	41.0	38.0	41.0
17	39.74226012058465	41.0	40.0	41.0	38.0	41.0
18	39.73537522209718	41.0	40.0	41.0	38.0	41.0
19	39.74529010906924	41.0	40.0	41.0	38.0	41.0
20	39.721560678456854	41.0	40.0	41.0	38.0	41.0
21	39.690377731867635	41.0	40.0	41.0	37.0	41.0
22	39.63941767237588	41.0	40.0	41.0	37.0	41.0
23	39.57453805155973	41.0	40.0	41.0	37.0	41.0
24	39.541716829099094	41.0	39.0	41.0	37.0	41.0
25	39.526799143177456	41.0	39.0	41.0	37.0	41.0
26	39.45550431166083	41.0	39.0	41.0	37.0	41.0
27	39.38483684665683	41.0	39.0	41.0	36.0	41.0
28	39.2866462252535	41.0	39.0	41.0	36.0	41.0
29	39.206472072449415	41.0	39.0	41.0	36.0	41.0
30	39.14650758364659	41.0	39.0	41.0	35.0	41.0
31	39.090736496628324	40.0	39.0	41.0	35.0	41.0
32	39.00673848252795	40.0	39.0	41.0	35.0	41.0
33	38.90384398207576	40.0	39.0	41.0	35.0	41.0
34	38.854954445155045	40.0	38.0	41.0	35.0	41.0
35	38.75253511167337	40.0	38.0	41.0	35.0	41.0
36	38.69465119061136	40.0	38.0	41.0	35.0	41.0
37	38.62485735097915	40.0	38.0	41.0	35.0	41.0
38	38.52488477733664	40.0	38.0	41.0	35.0	41.0
39	38.438337420905704	40.0	38.0	41.0	35.0	41.0
40	38.328115425697554	40.0	38.0	41.0	35.0	41.0
41	38.228488040616575	40.0	37.0	41.0	34.0	41.0
42	38.099364079873105	40.0	37.0	41.0	34.0	41.0
43	37.179714479746956	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	3.0
13	5.0
14	2.0
15	6.0
16	15.0
17	28.0
18	56.0
19	111.0
20	241.0
21	444.0
22	856.0
23	1365.0
24	2181.0
25	3404.0
26	5145.0
27	7075.0
28	10472.0
29	14513.0
30	19437.0
31	25458.0
32	33219.0
33	45462.0
34	67663.0
35	98756.0
36	161266.0
37	309153.0
38	691561.0
39	1787257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76420546816427	18.243611195920078	12.950111120885705	26.04207221502995
2	19.62759207235201	19.96845816689045	33.65737630514912	26.746573455608413
3	20.28978184022031	21.410457508779633	28.564231707365334	29.735528943634726
4	14.864842240608603	15.643525538017771	32.94791335949745	36.54371886187617
5	16.584788386322618	34.5387334136744	31.80328148300799	17.073196716994996
6	34.83028685656502	33.544809523795024	15.794477043895261	15.830426575744688
7	30.559476384871243	29.28815360951868	20.03146877732933	20.120901228280744
8	28.425243085498703	31.452370218689435	19.349523508893476	20.77286318691839
9	27.390347078879845	14.258540144607828	19.389551988471798	38.96156078804053
10	19.32900701061531	24.739244134457948	29.700309969587487	26.23143888533926
11	37.58025862354996	21.163133252829923	19.879299675114858	21.377308448505264
12	23.90628648032775	25.043582338886893	26.321480134459165	24.728651046326192
13	31.059573640378662	18.051474493944134	24.448908287904306	26.440043577772897
14	24.217717183419627	20.024498038300102	23.22336713482231	32.534417643457964
15	27.125672075128126	26.061797275689074	20.806042687101183	26.006487962081614
16	26.71573782837432	24.620650251235784	22.843659717134106	25.81995220325579
17	25.276112799342254	25.30174320223575	23.542894853555122	25.879249144866872
18	25.876418233383387	22.82825712347127	25.296446658169874	25.998877984975472
19	25.77194846767023	24.42419108226355	24.49937765607226	25.30448279399396
20	26.352741920411205	23.094697641911612	24.426200116219572	26.12636032145761
21	28.21983458344964	23.44070808097381	23.973710877488198	24.365746458088356
22	27.626347688895613	23.07527698033673	23.48429802983781	25.81407730092985
23	25.821504638585445	23.14486261099532	24.402883146366342	26.630749604052887
24	25.397294074780508	24.185085601588476	24.37767890219079	26.039941421440226
25	25.763090454318675	23.283820792953527	24.245234860635446	26.707853892092352
26	25.979457323400588	24.275309490158932	24.450034564516017	25.295198621924463
27	26.487803794091064	22.994946062004267	24.187520794262444	26.32972934964222
28	25.11800791498499	23.516290373592042	25.28898888060585	26.076712830817115
29	24.59760724055838	23.881021356335353	25.474489682545236	26.046881720561032
30	24.258537100616987	24.579860773946848	25.743700232652223	25.417901892783945
31	25.826892502376598	23.991853062909247	23.881964993496513	26.299289441217645
32	24.889739041709067	23.443082393830927	24.685456816271714	26.981721748188292
33	24.46994226462569	22.9834702165282	25.651558629851017	26.895028888995093
34	25.91824266755876	22.606228431422387	25.350903654341444	26.124625246677407
35	25.451659751226803	22.56869602433488	26.140636638508735	25.83900758592958
36	24.013587157524398	23.08090836339528	26.199537861310297	26.705966617770027
37	24.74849786661902	21.91795166203422	26.78520169939921	26.548348771947555
38	23.638597925642	21.142555874734906	28.11308182460464	27.105764375018452
39	24.112334220453732	20.909690575286874	28.385975603630992	26.5919996006284
40	23.263334734583786	21.723775486193826	28.65597759135701	26.356912187865372
41	22.27893853604131	21.915912188169774	29.74803974599723	26.05710952979169
42	22.90840540235319	22.176873523093693	29.305565237137543	25.60915583741558
43	21.166329443214504	21.666030979912403	29.578702535431294	27.588937041441802
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	338.0
1	359.0
2	380.0
3	1009.5
4	1639.0
5	1639.0
6	2105.5
7	2572.0
8	2545.5
9	2519.0
10	3811.5
11	5104.0
12	5104.0
13	8049.0
14	10994.0
15	16533.5
16	22073.0
17	20313.0
18	18553.0
19	18553.0
20	20802.0
21	23051.0
22	20191.5
23	17332.0
24	19300.5
25	21269.0
26	21269.0
27	24568.5
28	27868.0
29	32987.0
30	38106.0
31	44286.0
32	50466.0
33	50466.0
34	62694.0
35	74922.0
36	91324.0
37	107726.0
38	123070.0
39	138414.0
40	138414.0
41	150619.5
42	162825.0
43	176815.0
44	190805.0
45	205740.5
46	220676.0
47	220676.0
48	236781.0
49	252886.0
50	256212.5
51	259539.0
52	260958.5
53	262378.0
54	262378.0
55	251168.5
56	239959.0
57	230857.0
58	221755.0
59	217258.0
60	212761.0
61	212761.0
62	198777.0
63	184793.0
64	170728.5
65	156664.0
66	139054.5
67	121445.0
68	121445.0
69	102847.0
70	84249.0
71	71898.0
72	59547.0
73	45058.0
74	30569.0
75	30569.0
76	24426.5
77	18284.0
78	15588.5
79	12893.0
80	11815.0
81	10737.0
82	10737.0
83	8838.5
84	6940.0
85	6198.0
86	5456.0
87	4681.0
88	3906.0
89	3906.0
90	3011.0
91	2116.0
92	1259.5
93	403.0
94	286.0
95	169.0
96	169.0
97	116.0
98	63.0
99	40.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3285161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.44530928240549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.3293309836618	39.631471683969174
2	7.698252485673201	7.150954352622774
3	2.352711686669291	3.278172658190553
4	1.1841540460070066	2.1999360361922897
5	0.6817866803903089	1.5832896617676218
6	0.44804904044493915	1.2485865754290122
7	0.32627657223181467	1.0607811416236823
8	0.24847000272442304	0.923221289914877
9	0.18288580477076682	0.7644768989345905
>10	1.2556145642031542	11.412847666621015
>50	0.13993549820945603	4.545452473844288
>100	0.1248020872099071	11.703973154594578
>500	0.017808005817330972	5.870048140993742
>1k	0.009659693192373196	7.666713268444855
>5k	1.9713659576271828E-4	0.6462354319690263
>10k+	6.571219858757276E-5	0.31383956488795206
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10283	0.31301357832995097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8257	0.25134232386175287	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7485	0.22784271455797753	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	5432	0.16534958256231583	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	4505	0.1371317874527306	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	4189	0.1275127763905635	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	3838	0.11682836853353609	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3613	0.10997938913800571	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3310	0.10075609688535814	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.0439908424579495E-5
3	0.0	0.0	0.0	0.0	3.0439908424579495E-5
4	0.0	0.0	0.0	0.0	3.0439908424579495E-5
5	0.0	3.0439908424579495E-5	0.0	0.0	3.0439908424579495E-5
6	0.0	3.0439908424579495E-5	0.0	0.0	6.087981684915899E-5
7	0.0	6.087981684915899E-5	0.0	0.0	6.087981684915899E-5
8	0.0	6.087981684915899E-5	0.0	0.0	9.131972527373848E-5
9	0.0	6.087981684915899E-5	0.0	3.0439908424579495E-5	9.131972527373848E-5
10	0.0	6.087981684915899E-5	0.0	1.521995421228975E-4	9.131972527373848E-5
11	0.0	6.087981684915899E-5	0.0	1.8263945054747697E-4	9.131972527373848E-5
12	0.0	6.087981684915899E-5	0.0	4.2615871794411295E-4	9.131972527373848E-5
13	0.0	9.131972527373848E-5	0.0	6.392380769161694E-4	9.131972527373848E-5
14	0.0	9.131972527373848E-5	0.0	7.609977106144874E-4	9.131972527373848E-5
15	0.0	9.131972527373848E-5	0.0	0.0013089160622569184	9.131972527373848E-5
16	0.0	9.131972527373848E-5	0.0	0.002617832124513837	9.131972527373848E-5
17	0.0	9.131972527373848E-5	0.0	0.0037745486446478576	9.131972527373848E-5
18	0.0	9.131972527373848E-5	0.0	0.004261587179441129	9.131972527373848E-5
19	0.0	9.131972527373848E-5	0.0	0.004931265164781878	9.131972527373848E-5
20	0.0	9.131972527373848E-5	0.0	0.005418303699575151	9.131972527373848E-5
21	0.0	9.131972527373848E-5	0.0	0.006453260586010853	1.521995421228975E-4
22	0.0	9.131972527373848E-5	0.0	0.008279655091485623	1.521995421228975E-4
23	0.0	9.131972527373848E-5	0.0	0.011384525750792731	1.521995421228975E-4
24	0.0	1.2175963369831798E-4	0.0	0.017655146886256107	1.521995421228975E-4
25	0.0	1.2175963369831798E-4	0.0	0.021216616171931907	1.521995421228975E-4
26	0.0	1.2175963369831798E-4	0.0	0.03202278366265763	1.521995421228975E-4
27	0.0	1.2175963369831798E-4	0.0	0.07673900913836491	1.521995421228975E-4
28	0.0	1.2175963369831798E-4	0.0	0.26570996063815444	1.521995421228975E-4
29	0.0	1.521995421228975E-4	0.0	0.5207963932361306	1.521995421228975E-4
30	0.0	1.521995421228975E-4	0.0	0.8910065594958664	1.521995421228975E-4
31	0.0	1.521995421228975E-4	0.0	1.8449324097053386	1.521995421228975E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	35	8.87292E-4	26.42857	2
GGTATCA	5315	0.0	19.42239	1
CTTATAC	6510	0.0	18.812595	37
TCTAGAC	535	0.0	18.327103	3
AGTCGGT	1610	0.0	18.155277	11
GCAGTCG	1640	0.0	17.935974	9
ATACACA	1405	0.0	17.907475	37
CTAGACA	595	0.0	17.722689	4
GTTCTAG	545	0.0	16.972477	1
CAGTCGG	1755	0.0	16.97151	10
GGCAGTC	1870	0.0	16.32353	8
GTCTAGA	495	0.0	15.69697	1
TCGGTGA	1840	0.0	15.584239	13
AAGACGG	1000	0.0	15.54	5
GTCGGTG	1860	0.0	15.516129	12
CTCTAGT	515	0.0	15.446603	27
TCTAGTT	540	0.0	15.416667	28
ACTGATC	565	0.0	15.38938	8
CTCTATG	2995	0.0	15.380633	1
CGTATCT	135	3.9783845E-7	15.074075	27
>>END_MODULE