FastQCFastQC Report
Fri 10 Feb 2017
ERR1631424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804163
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12470.15506806455905084No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9610.11950313555833829No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9300.1156481957016177No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8720.1084357275825921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA451.3227294E-424.66666624
GGTATCA8700.022.540231
ATACACA5050.021.61386137
TATACAC9400.019.8776637
CCTAAGG759.2600985E-619.7333343
TACCGTC1900.019.4736827
TCTATGG3100.019.0967752
TAACGAA1750.019.02857213
ATACCGT1950.018.974366
GTCCTAA1002.872257E-718.51
CTATAGG609.233525E-418.52
TCCTAAG801.6156868E-518.52
CTCTATG3150.018.206351
CAGTCGG2550.018.13725510
GTTTTAT1451.546141E-1017.8620681
TACACAT1451.546141E-1017.86206837
GCCCCTA1258.56744E-917.7627
ATAACGA2000.017.57512
ACCGTCG2000.017.5758
CTTATAC30700.017.41531237
Produced by FastQC (version 0.11.5)