##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804163
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82694553218688	34.0	31.0	34.0	31.0	34.0
2	33.003251828298495	34.0	33.0	34.0	31.0	34.0
3	33.11601752381047	34.0	33.0	34.0	31.0	34.0
4	36.44565591801662	37.0	37.0	37.0	35.0	37.0
5	36.42565499780517	37.0	37.0	37.0	35.0	37.0
6	36.472483812361425	37.0	37.0	37.0	35.0	37.0
7	36.46183796071195	37.0	37.0	37.0	35.0	37.0
8	36.43747349728849	37.0	37.0	37.0	35.0	37.0
9	38.29467533323468	39.0	39.0	39.0	37.0	39.0
10	38.25222871482523	39.0	39.0	39.0	37.0	39.0
11	38.31494982982306	39.0	39.0	39.0	37.0	39.0
12	38.09009118798055	39.0	39.0	39.0	37.0	39.0
13	38.21309858822154	39.0	39.0	39.0	37.0	39.0
14	39.738201086098215	41.0	40.0	41.0	38.0	41.0
15	39.74990891150177	41.0	40.0	41.0	38.0	41.0
16	39.72696704523834	41.0	40.0	41.0	38.0	41.0
17	39.716781299313695	41.0	40.0	41.0	38.0	41.0
18	39.68973081328039	41.0	40.0	41.0	37.0	41.0
19	39.703897095489346	41.0	40.0	41.0	37.0	41.0
20	39.686053698068676	41.0	40.0	41.0	37.0	41.0
21	39.64680419268233	41.0	40.0	41.0	37.0	41.0
22	39.593811204942284	41.0	40.0	41.0	37.0	41.0
23	39.532390821263846	41.0	39.0	41.0	37.0	41.0
24	39.49509116932761	41.0	39.0	41.0	37.0	41.0
25	39.463973099980976	41.0	39.0	41.0	37.0	41.0
26	39.3947358930963	41.0	39.0	41.0	37.0	41.0
27	39.33069539384428	41.0	39.0	41.0	36.0	41.0
28	39.2305067504971	41.0	39.0	41.0	36.0	41.0
29	39.169146056209996	41.0	39.0	41.0	36.0	41.0
30	39.11396321392554	41.0	39.0	41.0	35.0	41.0
31	39.07412303227082	40.0	39.0	41.0	35.0	41.0
32	39.00062176449302	40.0	39.0	41.0	35.0	41.0
33	38.91833247737088	40.0	39.0	41.0	35.0	41.0
34	38.87910784256426	40.0	39.0	41.0	35.0	41.0
35	38.78057931041344	40.0	38.0	41.0	35.0	41.0
36	38.73863507771434	40.0	38.0	41.0	35.0	41.0
37	38.68357783185747	40.0	38.0	41.0	35.0	41.0
38	38.60607737486057	40.0	38.0	41.0	35.0	41.0
39	38.535546897830415	40.0	38.0	41.0	35.0	41.0
40	38.45286589907767	40.0	38.0	41.0	35.0	41.0
41	38.37589021131288	40.0	38.0	41.0	34.0	41.0
42	38.2867105300791	40.0	37.0	41.0	34.0	41.0
43	37.30506626144202	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	3.0
16	1.0
17	11.0
18	26.0
19	36.0
20	84.0
21	121.0
22	246.0
23	386.0
24	575.0
25	852.0
26	1242.0
27	1741.0
28	2404.0
29	3304.0
30	4413.0
31	5839.0
32	7940.0
33	11031.0
34	16587.0
35	24485.0
36	40198.0
37	73543.0
38	177370.0
39	431722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75916325421587	16.539805984607597	12.34389047991514	27.357140281261387
2	20.94625094663644	18.683649956538662	31.609387649021404	28.760711447803494
3	20.680135743624117	19.631965161291927	27.98661465399428	31.701284441089676
4	15.78610306616942	14.952192528131736	31.861077915795676	37.40062648990317
5	17.518587649518818	33.01134222788166	32.09125513111148	17.378814991488046
6	36.877846904172415	32.311732820336175	14.907549837532938	15.902870437958475
7	32.22207438044277	27.76514214158075	19.255673290116558	20.757110187859922
8	29.685897013416433	29.968924210638885	18.90474941025638	21.4404293656883
9	27.821847063344123	13.848684905920813	17.782837559052084	40.54663047168298
10	19.896339423723795	23.388293169419633	29.40000969952609	27.315357707330477
11	38.97232277535773	20.09915900134674	18.884231181986735	22.044287041308788
12	25.121150811464837	23.20052029252776	25.3500844977946	26.328244398212803
13	32.57560967117363	16.54589927663919	23.246406512112593	27.632084540074587
14	25.214539838316362	18.617121155785583	21.55135215124297	34.61698685465509
15	28.19789022872229	24.500505494532828	19.4009174756859	27.90068680105899
16	28.2623050301991	23.230489341091296	21.139495351066888	27.367710277642715
17	27.444809074777126	23.47073914119401	21.706793274497834	27.377658509531027
18	27.17471457900948	21.418294549736807	23.579547927472415	27.8274429437813
19	27.941723257598273	22.610590141550905	22.392475157399684	27.055211443451142
20	28.70189252676385	21.247184961257854	21.91844190792165	28.132480604056642
21	29.884115533791032	21.728679384652118	21.705301039714588	26.681904041842262
22	29.738373936627276	21.613155541849103	21.198438625005128	27.45003189651849
23	28.006262411973694	21.462937240335602	22.057219742763596	28.47358060492711
24	26.866070684674625	22.467459955257826	22.77473596770804	27.89173339235951
25	27.463586362466312	21.619248833880693	22.27769743198829	28.6394673716647
26	27.677722053862215	22.76366855973229	22.54468310529084	27.013926281114646
27	28.469476959273184	21.248801548939706	22.066173151463072	28.215548340324037
28	27.165263758715586	22.148370417440244	22.664061887950577	28.022303935893593
29	26.423871777239192	22.347335055206468	23.646947198515726	27.581845969038614
30	26.214585848888845	22.927938738788033	23.36740188245418	27.490073529868948
31	27.224331385552432	22.36872375376634	22.38961504073179	28.01732981994944
32	25.808200576251334	21.484325938895473	23.62506108836144	29.08241239649176
33	25.68533991243069	21.281506361272527	24.458847273500524	28.57430645279626
34	27.29794830152593	20.23333577894034	24.67459955257827	27.794116366955457
35	26.25238913006443	19.99930362376782	25.678127444311666	28.070179801856092
36	24.58158358442256	20.612487766783598	26.282358178627963	28.523570470165875
37	25.296239692699118	18.874780361692842	26.98196758617345	28.847012359434594
38	24.059425763184827	18.52597048110893	28.33554888747679	29.079054868229452
39	24.03915624071239	18.13624849688434	29.636653265569297	28.187941996833977
40	22.855067940206155	18.81310132398531	30.044282067192846	28.287548668615692
41	21.225423204002173	18.91270799576703	31.955213060038822	27.906655740191976
42	21.621611538954166	19.389352656115737	31.39152137066739	27.597514434262706
43	19.953168698385777	19.142139093691206	31.648807517878836	29.25588469004418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	12.0
2	11.0
3	29.0
4	47.0
5	47.0
6	59.0
7	71.0
8	82.0
9	93.0
10	142.5
11	192.0
12	192.0
13	302.5
14	413.0
15	592.0
16	771.0
17	779.0
18	787.0
19	787.0
20	987.5
21	1188.0
22	1255.0
23	1322.0
24	1781.5
25	2241.0
26	2241.0
27	2829.0
28	3417.0
29	4518.5
30	5620.0
31	7322.0
32	9024.0
33	9024.0
34	11232.0
35	13440.0
36	16857.5
37	20275.0
38	23930.5
39	27586.0
40	27586.0
41	30348.5
42	33111.0
43	36957.0
44	40803.0
45	44268.0
46	47733.0
47	47733.0
48	52119.0
49	56505.0
50	60135.5
51	63766.0
52	68427.0
53	73088.0
54	73088.0
55	70032.0
56	66976.0
57	65716.5
58	64457.0
59	63084.5
60	61712.0
61	61712.0
62	58194.5
63	54677.0
64	51111.0
65	47545.0
66	42085.0
67	36625.0
68	36625.0
69	31614.0
70	26603.0
71	22468.5
72	18334.0
73	13978.5
74	9623.0
75	9623.0
76	7695.0
77	5767.0
78	4853.0
79	3939.0
80	3361.5
81	2784.0
82	2784.0
83	2209.5
84	1635.0
85	1298.0
86	961.0
87	774.5
88	588.0
89	588.0
90	453.0
91	318.0
92	189.0
93	60.0
94	44.5
95	29.0
96	29.0
97	19.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	804163.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.025382882528156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.96064000118304	50.73014557542213
2	5.443294278370263	6.20811880732678
3	1.713334300482091	2.9311063347227937
4	0.8658687161182917	1.975059802505947
5	0.5362134176915216	1.5288887725304008
6	0.3773918873997096	1.2912550125437037
7	0.26564354963582026	1.0603897589779567
8	0.20753499897036837	0.9467810222248275
9	0.17035426460636469	0.8743065448364515
>10	1.1937946612359596	13.512374729734653
>50	0.1544888900964738	6.139055194352619
>100	0.10838186856543496	11.559187716341947
>500	0.0028406538129341773	1.0879455392220592
>1k	2.1851183176416748E-4	0.15538518925780642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1247	0.15506806455905084	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	961	0.11950313555833829	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	930	0.1156481957016177	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	872	0.1084357275825921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.974115944155601E-4	0.0
2	0.0	0.0	0.0	4.974115944155601E-4	0.0
3	0.0	0.0	0.0	4.974115944155601E-4	0.0
4	0.0	0.0	0.0	4.974115944155601E-4	0.0
5	0.0	0.0	0.0	4.974115944155601E-4	1.2435289860389002E-4
6	0.0	1.2435289860389002E-4	0.0	4.974115944155601E-4	1.2435289860389002E-4
7	0.0	1.2435289860389002E-4	0.0	4.974115944155601E-4	1.2435289860389002E-4
8	0.0	1.2435289860389002E-4	0.0	4.974115944155601E-4	2.4870579720778004E-4
9	0.0	1.2435289860389002E-4	0.0	4.974115944155601E-4	2.4870579720778004E-4
10	0.0	1.2435289860389002E-4	0.0	4.974115944155601E-4	2.4870579720778004E-4
11	0.0	1.2435289860389002E-4	0.0	4.974115944155601E-4	2.4870579720778004E-4
12	0.0	1.2435289860389002E-4	0.0	6.2176449301945E-4	2.4870579720778004E-4
13	0.0	1.2435289860389002E-4	0.0	6.2176449301945E-4	2.4870579720778004E-4
14	0.0	1.2435289860389002E-4	0.0	6.2176449301945E-4	2.4870579720778004E-4
15	0.0	1.2435289860389002E-4	0.0	6.2176449301945E-4	2.4870579720778004E-4
16	0.0	1.2435289860389002E-4	0.0	8.704702902272301E-4	2.4870579720778004E-4
17	0.0	1.2435289860389002E-4	0.0	9.948231888311202E-4	2.4870579720778004E-4
18	0.0	1.2435289860389002E-4	0.0	0.0012435289860389	2.4870579720778004E-4
19	0.0	1.2435289860389002E-4	0.0	0.00149223478324668	2.4870579720778004E-4
20	0.0	1.2435289860389002E-4	0.0	0.0017409405804544602	2.4870579720778004E-4
21	0.0	1.2435289860389002E-4	0.0	0.00236270507347391	2.4870579720778004E-4
22	0.0	1.2435289860389002E-4	0.0	0.00323317536370114	3.7305869581167E-4
23	0.0	1.2435289860389002E-4	0.0	0.00634199782879839	3.7305869581167E-4
24	0.0	1.2435289860389002E-4	0.0	0.012310936961785111	3.7305869581167E-4
25	0.0	1.2435289860389002E-4	0.0	0.01666328841292126	3.7305869581167E-4
26	0.0	1.2435289860389002E-4	0.0	0.029844695664933602	3.7305869581167E-4
27	0.0	1.2435289860389002E-4	0.0	0.11751348918067606	3.7305869581167E-4
28	0.0	1.2435289860389002E-4	0.0	0.5377019335632204	3.7305869581167E-4
29	0.0	1.2435289860389002E-4	0.0	1.131735730194003	3.7305869581167E-4
30	0.0	1.2435289860389002E-4	0.0	1.897003468202342	3.7305869581167E-4
31	0.0	1.2435289860389002E-4	0.0	3.6241408769117704	3.7305869581167E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	45	1.3227294E-4	24.666666	24
GGTATCA	870	0.0	22.54023	1
ATACACA	505	0.0	21.613861	37
TATACAC	940	0.0	19.87766	37
CCTAAGG	75	9.2600985E-6	19.733334	3
TACCGTC	190	0.0	19.473682	7
TCTATGG	310	0.0	19.096775	2
TAACGAA	175	0.0	19.028572	13
ATACCGT	195	0.0	18.97436	6
GTCCTAA	100	2.872257E-7	18.5	1
CTATAGG	60	9.233525E-4	18.5	2
TCCTAAG	80	1.6156868E-5	18.5	2
CTCTATG	315	0.0	18.20635	1
CAGTCGG	255	0.0	18.137255	10
GTTTTAT	145	1.546141E-10	17.862068	1
TACACAT	145	1.546141E-10	17.862068	37
GCCCCTA	125	8.56744E-9	17.76	27
ATAACGA	200	0.0	17.575	12
ACCGTCG	200	0.0	17.575	8
CTTATAC	3070	0.0	17.415312	37
>>END_MODULE