FastQCFastQC Report
Fri 10 Feb 2017
ERR1631423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855505
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA37230.20064618527031725No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC35150.18943629901293718No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC27370.14750701291562135No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC25570.13780614980827322No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC25370.13672827612967897No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC23100.12449440987763438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG451.3236406E-424.6666665
GGTATCA15450.021.1941741
CTTATAC23800.021.14285737
ATAATAC806.964856E-720.81253
GTCTAGA3000.019.7333341
ACTGATC2450.018.877558
GTATAAT609.2397333E-418.51
TATACAC6300.018.20634837
GCAGTCG8500.018.0647059
AGTCGGT8500.017.6294111
ATACACA4150.016.93975837
CTAGACT1005.881706E-616.6500024
CTCTATG12450.016.6425691
CTAGAAC3450.016.6231883
TAGGCCG1352.2217137E-816.4444455
CAGTCGG9500.015.96842110
TAGATAA3500.015.8571434
TATTGAC2250.015.62222230
TCTAGAC3100.015.5161293
TCTATGG13250.015.4981142
Produced by FastQC (version 0.11.5)