##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631423.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1855505
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.868794209662596	34.0	33.0	34.0	31.0	34.0
2	33.04679319107197	34.0	33.0	34.0	31.0	34.0
3	33.160206520596816	34.0	33.0	34.0	31.0	34.0
4	36.47325067838675	37.0	37.0	37.0	35.0	37.0
5	36.450822282882555	37.0	37.0	37.0	35.0	37.0
6	36.49573512332222	37.0	37.0	37.0	35.0	37.0
7	36.48059369282217	37.0	37.0	37.0	35.0	37.0
8	36.461750305172984	37.0	37.0	37.0	35.0	37.0
9	38.32122468007362	39.0	39.0	39.0	37.0	39.0
10	38.27930240015522	39.0	39.0	39.0	37.0	39.0
11	38.34232836882681	39.0	39.0	39.0	37.0	39.0
12	38.11352542838742	39.0	39.0	39.0	37.0	39.0
13	38.24211468037004	39.0	39.0	39.0	37.0	39.0
14	39.78194669375723	41.0	40.0	41.0	38.0	41.0
15	39.79199032069437	41.0	40.0	41.0	38.0	41.0
16	39.78072061244782	41.0	40.0	41.0	38.0	41.0
17	39.76259886122646	41.0	40.0	41.0	38.0	41.0
18	39.74906507931803	41.0	40.0	41.0	38.0	41.0
19	39.75284087081415	41.0	40.0	41.0	38.0	41.0
20	39.73260001994066	41.0	40.0	41.0	38.0	41.0
21	39.695311518966534	41.0	40.0	41.0	37.0	41.0
22	39.65548624228983	41.0	40.0	41.0	37.0	41.0
23	39.598455945955415	41.0	40.0	41.0	37.0	41.0
24	39.565877214019906	41.0	40.0	41.0	37.0	41.0
25	39.542619394720035	41.0	39.0	41.0	37.0	41.0
26	39.48733148118706	41.0	39.0	41.0	37.0	41.0
27	39.41451734164015	41.0	39.0	41.0	37.0	41.0
28	39.3220966798796	41.0	39.0	41.0	36.0	41.0
29	39.26113807292354	41.0	39.0	41.0	36.0	41.0
30	39.21034435369347	41.0	39.0	41.0	36.0	41.0
31	39.17852929525924	41.0	39.0	41.0	35.0	41.0
32	39.10632684902493	41.0	39.0	41.0	35.0	41.0
33	39.016771175502086	40.0	39.0	41.0	35.0	41.0
34	38.97452607241694	40.0	39.0	41.0	35.0	41.0
35	38.88196852069922	40.0	39.0	41.0	35.0	41.0
36	38.832630469872086	40.0	38.0	41.0	35.0	41.0
37	38.77265111115303	40.0	38.0	41.0	35.0	41.0
38	38.687223693819206	40.0	38.0	41.0	35.0	41.0
39	38.60647586506099	40.0	38.0	41.0	35.0	41.0
40	38.52373019743951	40.0	38.0	41.0	35.0	41.0
41	38.44290853433432	40.0	38.0	41.0	35.0	41.0
42	38.33769728456674	40.0	37.0	41.0	34.0	41.0
43	37.3863363343133	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	2.0
15	1.0
16	4.0
17	14.0
18	31.0
19	43.0
20	129.0
21	217.0
22	428.0
23	741.0
24	1066.0
25	1714.0
26	2484.0
27	3617.0
28	5089.0
29	6960.0
30	9437.0
31	12812.0
32	17627.0
33	23811.0
34	36086.0
35	54128.0
36	89055.0
37	165820.0
38	402462.0
39	1021724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.513598723797564	16.851584878510163	11.857203295059836	27.777613102632436
2	20.94071425299312	18.58954839787551	31.965583493442484	28.504153855688884
3	21.18215795699823	19.365294084359782	27.34527797014829	32.1072699884937
4	15.637252392205895	14.261023279376772	31.451114386649458	38.650609941767875
5	17.984591795764494	33.20055726069183	31.28237326226553	17.53247768127814
6	38.30951681617673	31.615651803686866	14.362343405164632	15.712487974971772
7	32.95388586934554	27.29246215989717	18.77661337479554	20.977038595961748
8	29.538373650300052	30.33255097668829	18.255730919614876	21.873344453396783
9	27.74333671965314	13.372424218743683	17.661229692186225	41.22300936941695
10	19.418056000926974	23.788672086574813	29.54268514501443	27.250586767483785
11	40.3011040121153	19.55521542652809	18.467263629038992	21.676416932317615
12	25.158218382596655	22.943026292033704	25.449136488449238	26.449618836920408
13	33.0111209616789	16.615961692369464	22.845263149385207	27.52765419656643
14	24.954769725761988	18.347889119134685	21.401074101120717	35.29626705398261
15	28.644385221273993	24.48557131346992	19.356239945459592	27.513803519796497
16	28.722315488236354	22.813789237970255	20.984422030660117	27.479473243133274
17	27.301192936693784	23.396972791773667	21.620906437870012	27.680927833662533
18	28.010649391944508	20.984799286447625	23.16652339929022	27.838027922317643
19	27.581979029967584	22.60198706012649	22.02004306105346	27.79599084885247
20	28.100166800951765	21.4291527104481	22.361351761380323	28.10932872721981
21	29.712019099921584	21.88676398069528	22.13990261411314	26.261314305269995
22	29.394315833155932	21.47582464073123	21.265908741825	27.863950784287834
23	27.71870730609726	21.441602151435863	22.376388099196713	28.463302443270162
24	27.266000361087684	22.28552334809122	22.110907812158953	28.337568478662146
25	27.890143114677674	21.533167520432443	22.048930075639785	28.5277592892501
26	28.347754384924862	22.247582194604703	22.062187921886494	27.342475498583944
27	28.419379090867448	21.257986370287334	22.33160244785112	27.991032090994096
28	27.293054990420394	21.66267404291554	22.896084893330926	28.148186073333136
29	26.545280125895648	22.04806777669691	23.272047232424594	28.134604864982848
30	25.943611038504343	23.070215386107826	23.717855785891174	27.26831778949666
31	28.105663956712597	22.245534234615373	21.77003026130353	27.87877154736851
32	26.876780175747307	21.812714058975857	22.540709941498406	28.76979582377843
33	26.575514482580214	21.376067431777333	23.29387417441613	28.75454391122632
34	27.905287239861927	21.081861811205034	23.143618583620093	27.869232365312946
35	27.26271284636797	21.125192333084524	24.066386239864617	27.545708580682888
36	26.096237951393285	21.58668394857465	24.257601030447237	28.059477069584833
37	27.04336555277404	20.22037127358859	24.706589311265667	28.029673862371702
38	25.417662577034285	19.742819340287415	26.124586029140318	28.714932053537982
39	26.138113343806673	19.332203362426938	26.509925869237755	28.01975742452863
40	25.090258447161283	20.153058062360383	26.76732210368606	27.98936138679228
41	24.307291007030432	19.92384822460732	28.324095057679717	27.444765710682535
42	24.792819205553204	19.940986416096965	28.184618203669622	27.08157617468021
43	22.613951457958883	19.581946693757224	28.53401095658594	29.270090891697947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	14.0
2	16.0
3	55.0
4	94.0
5	94.0
6	132.0
7	170.0
8	162.5
9	155.0
10	237.5
11	320.0
12	320.0
13	537.5
14	755.0
15	1264.5
16	1774.0
17	1687.5
18	1601.0
19	1601.0
20	1924.0
21	2247.0
22	2138.0
23	2029.0
24	2546.0
25	3063.0
26	3063.0
27	4121.0
28	5179.0
29	6877.0
30	8575.0
31	10975.5
32	13376.0
33	13376.0
34	18373.5
35	23371.0
36	31012.5
37	38654.0
38	47269.0
39	55884.0
40	55884.0
41	63196.5
42	70509.0
43	80350.0
44	90191.0
45	102567.0
46	114943.0
47	114943.0
48	127030.5
49	139118.0
50	145594.5
51	152071.0
52	158696.0
53	165321.0
54	165321.0
55	161448.0
56	157575.0
57	155021.0
58	152467.0
59	150559.5
60	148652.0
61	148652.0
62	141202.5
63	133753.0
64	124945.0
65	116137.0
66	104918.0
67	93699.0
68	93699.0
69	77623.5
70	61548.0
71	52472.0
72	43396.0
73	32707.0
74	22018.0
75	22018.0
76	17322.5
77	12627.0
78	10477.0
79	8327.0
80	7455.0
81	6583.0
82	6583.0
83	5288.0
84	3993.0
85	3260.0
86	2527.0
87	2040.0
88	1553.0
89	1553.0
90	1215.5
91	878.0
92	543.0
93	208.0
94	148.5
95	89.0
96	89.0
97	63.5
98	38.0
99	23.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1855505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.43039316726605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.79214402735877	40.21724728713076
2	7.467447341976492	7.083679267716017
3	2.4907503018998494	3.544117983017894
4	1.2844211552603708	2.436824015454138
5	0.7795309473977586	1.8486729660563541
6	0.5145905230007327	1.4644338495644291
7	0.4051952524272446	1.3452999092493678
8	0.29114464398963524	1.1047283946377682
9	0.21102682221882346	0.9008176632009794
>10	1.441804372374059	13.215299955250096
>50	0.1646301609670187	5.449765455594056
>100	0.13887417504993044	13.225094328397985
>500	0.01297649057440821	4.229535711162012
>1k	0.0054637855050139825	3.934483213568185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3723	0.20064618527031725	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3515	0.18943629901293718	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2737	0.14750701291562135	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2557	0.13780614980827322	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2537	0.13672827612967897	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	2310	0.12449440987763438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.3893683929711856E-5	0.0	0.0	0.0
3	0.0	5.3893683929711856E-5	0.0	0.0	0.0
4	0.0	5.3893683929711856E-5	0.0	0.0	0.0
5	0.0	5.3893683929711856E-5	0.0	0.0	0.0
6	0.0	5.3893683929711856E-5	0.0	0.0	0.0
7	0.0	5.3893683929711856E-5	0.0	0.0	0.0
8	0.0	5.3893683929711856E-5	0.0	0.0	0.0
9	0.0	5.3893683929711856E-5	0.0	1.0778736785942371E-4	0.0
10	0.0	5.3893683929711856E-5	0.0	1.0778736785942371E-4	0.0
11	0.0	5.3893683929711856E-5	0.0	1.0778736785942371E-4	0.0
12	0.0	5.3893683929711856E-5	0.0	1.0778736785942371E-4	0.0
13	0.0	5.3893683929711856E-5	0.0	1.0778736785942371E-4	0.0
14	0.0	5.3893683929711856E-5	0.0	1.6168105178913556E-4	0.0
15	0.0	5.3893683929711856E-5	0.0	2.694684196485593E-4	0.0
16	0.0	1.0778736785942371E-4	0.0	5.389368392971186E-4	0.0
17	0.0	1.0778736785942371E-4	0.0	0.0010239799946645254	0.0
18	0.0	1.0778736785942371E-4	0.0	0.0011856610464536608	0.0
19	0.0	1.0778736785942371E-4	0.0	0.001509023150031932	5.3893683929711856E-5
20	0.0	1.0778736785942371E-4	0.0	0.0017245978857507794	5.3893683929711856E-5
21	0.0	1.0778736785942371E-4	0.0	0.0023713220929073215	1.0778736785942371E-4
22	0.0	1.0778736785942371E-4	0.0	0.003071939983993576	1.0778736785942371E-4
23	0.0	1.6168105178913556E-4	0.0	0.0045270694500957965	1.0778736785942371E-4
24	0.0	1.6168105178913556E-4	0.0	0.008730776796613321	1.0778736785942371E-4
25	0.0	1.6168105178913556E-4	0.0	0.011048205205590932	1.0778736785942371E-4
26	0.0	1.6168105178913556E-4	0.0	0.017569340961086064	1.0778736785942371E-4
27	0.0	1.6168105178913556E-4	0.0	0.0409053061026513	1.0778736785942371E-4
28	0.0	1.6168105178913556E-4	0.0	0.14826152449063731	1.0778736785942371E-4
29	0.0	1.6168105178913556E-4	0.0	0.3136073467869933	1.0778736785942371E-4
30	0.0	1.6168105178913556E-4	0.0	0.5329546403809206	1.0778736785942371E-4
31	0.0	1.6168105178913556E-4	0.0	1.2194523862775901	1.0778736785942371E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCCG	45	1.3236406E-4	24.666666	5
GGTATCA	1545	0.0	21.194174	1
CTTATAC	2380	0.0	21.142857	37
ATAATAC	80	6.964856E-7	20.8125	3
GTCTAGA	300	0.0	19.733334	1
ACTGATC	245	0.0	18.87755	8
GTATAAT	60	9.2397333E-4	18.5	1
TATACAC	630	0.0	18.206348	37
GCAGTCG	850	0.0	18.064705	9
AGTCGGT	850	0.0	17.62941	11
ATACACA	415	0.0	16.939758	37
CTAGACT	100	5.881706E-6	16.650002	4
CTCTATG	1245	0.0	16.642569	1
CTAGAAC	345	0.0	16.623188	3
TAGGCCG	135	2.2217137E-8	16.444445	5
CAGTCGG	950	0.0	15.968421	10
TAGATAA	350	0.0	15.857143	4
TATTGAC	225	0.0	15.622222	30
TCTAGAC	310	0.0	15.516129	3
TCTATGG	1325	0.0	15.498114	2
>>END_MODULE