FastQCFastQC Report
Fri 10 Feb 2017
ERR1631422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030231
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.34681542294883383No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC31340.3042036203531053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30070.2918762879393068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26610.25829158703242283No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT25530.24780850120021627No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG14250.13831849361939216No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT13910.13501826289443825No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT13290.12900019510187521No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG13160.1277383421776281No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG12650.12278799609019725No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT11760.11414915683958257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.10725749856100235No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10990.10667510490365753No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG10720.10405433344560588No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG10420.10114236515888185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA250.00549608329.5999985
GGACCGT358.8687695E-426.428576
TTCGCTA400.001931052223.12526
GGTATCA15350.021.6938111
ATACACA3700.021.537
CCGATAT450.003825543220.55555519
TGCATCA7600.019.96052714
CAGGACA8750.019.451434
GCATCAG8150.019.29447715
CTGCATC8200.018.95122113
TCGCTAC500.007034418.49999827
AGTCGGT4750.018.30526411
GCTGCAT8450.018.17159712
TCGGTGA4900.018.12244813
CATTTGC2950.017.55932225
GCAGTCG5000.017.3900019
CAGTCGG4950.017.1919210
GACAGGC9200.017.0923927
GTATTAT650.001579783917.0769231
TCGTTTA2300.016.89130430
Produced by FastQC (version 0.11.5)