FastQCFastQC Report
Fri 10 Feb 2017
ERR1631421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661808
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC77180.4644339177570453No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT50680.304969045762206No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA32440.19520907349104108No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG29320.17643434139202602No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC29050.1748096049603805No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT29000.1745087278434091No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG26120.15717820590585677No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG25330.1524243474577087No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT24950.1501376813687261No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG23850.14351838479535542No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT23750.14291663056141263No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC22360.13455224670960783No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC22060.13274698400777948No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT21340.1284143535233914No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20390.12269768830093489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.11968891713122094No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA18410.11078295446886764No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG18300.11012102481153056No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT18290.1100608493881363No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC16980.10217786892348574No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG16850.10139558841936011No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC16840.10133541299596584No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG16670.10031243079826309No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT16660.10025225537486883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCA16600.024.18373514
GACAGGC16950.024.1209457
GCATCAG17050.023.2199415
CAGGACA20000.022.574
GGTATCA20100.022.5497531
ACAGGCT18350.022.1798368
GCTGCAT17950.021.95264412
CTGCATC18600.021.68279613
CCAGGAC20750.021.5759033
TCCAGGA20950.021.4582332
CATCAGA19650.020.2417316
CAGAAGA20150.020.106719
ATCAGAA19900.020.08040217
AGAAGAG20450.019.99266620
GGACAGG22100.019.9230776
AAGAGGC20800.019.47836522
TCTAGAT2950.019.4406782
GGCCATC20900.019.11961726
CCATCAA20800.019.03365328
TCAGAAG21400.019.01869218
Produced by FastQC (version 0.11.5)