##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631421.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1661808
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83260460895603	34.0	31.0	34.0	31.0	34.0
2	33.01336797030704	34.0	33.0	34.0	31.0	34.0
3	33.1059171697332	34.0	33.0	34.0	31.0	34.0
4	36.44774185706171	37.0	37.0	37.0	35.0	37.0
5	36.43386420091852	37.0	37.0	37.0	35.0	37.0
6	36.47489421160567	37.0	37.0	37.0	35.0	37.0
7	36.46415590730096	37.0	37.0	37.0	35.0	37.0
8	36.43970242049623	37.0	37.0	37.0	35.0	37.0
9	38.29689470745116	39.0	39.0	39.0	37.0	39.0
10	38.25857740485062	39.0	39.0	39.0	37.0	39.0
11	38.32143966089946	39.0	39.0	39.0	37.0	39.0
12	38.096558687886926	39.0	39.0	39.0	37.0	39.0
13	38.209512771631864	39.0	39.0	39.0	37.0	39.0
14	39.74424843303198	41.0	40.0	41.0	38.0	41.0
15	39.751316036509635	41.0	40.0	41.0	38.0	41.0
16	39.737012940123044	41.0	40.0	41.0	38.0	41.0
17	39.699659046051046	41.0	40.0	41.0	37.0	41.0
18	39.63940780162329	41.0	40.0	41.0	37.0	41.0
19	39.66493782675255	41.0	40.0	41.0	37.0	41.0
20	39.65510095029028	41.0	40.0	41.0	37.0	41.0
21	39.633862636357506	41.0	40.0	41.0	37.0	41.0
22	39.58415833838807	41.0	40.0	41.0	37.0	41.0
23	39.525380188325	41.0	39.0	41.0	37.0	41.0
24	39.49292818424271	41.0	39.0	41.0	37.0	41.0
25	39.464163730106	41.0	39.0	41.0	37.0	41.0
26	39.392531507851686	41.0	39.0	41.0	36.0	41.0
27	39.32842662930977	41.0	39.0	41.0	36.0	41.0
28	39.23561085275796	41.0	39.0	41.0	36.0	41.0
29	39.15545598528831	41.0	39.0	41.0	35.0	41.0
30	39.09571201967977	41.0	39.0	41.0	35.0	41.0
31	39.074402096993154	40.0	39.0	41.0	35.0	41.0
32	39.0092206801267	40.0	39.0	41.0	35.0	41.0
33	38.90522069938284	40.0	39.0	41.0	35.0	41.0
34	38.845508024994466	40.0	38.0	41.0	35.0	41.0
35	38.72309677170889	40.0	38.0	41.0	35.0	41.0
36	38.680731468376614	40.0	38.0	41.0	35.0	41.0
37	38.611237278915496	40.0	38.0	41.0	35.0	41.0
38	38.527587422855106	40.0	38.0	41.0	35.0	41.0
39	38.43985165554625	40.0	38.0	41.0	34.0	41.0
40	38.33239218971145	40.0	38.0	41.0	34.0	41.0
41	38.24086055669488	40.0	37.0	41.0	34.0	41.0
42	38.13689307067965	40.0	37.0	41.0	34.0	41.0
43	37.16045716472661	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	3.0
12	2.0
13	2.0
14	1.0
15	3.0
16	3.0
17	13.0
18	34.0
19	74.0
20	123.0
21	223.0
22	430.0
23	711.0
24	1099.0
25	1661.0
26	2520.0
27	3432.0
28	5025.0
29	6800.0
30	9518.0
31	12493.0
32	16829.0
33	23339.0
34	36644.0
35	57790.0
36	84555.0
37	152250.0
38	362122.0
39	884105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.204379808016334	14.836792216670036	11.336267486978038	28.622560488335598
2	21.187586050855455	17.417896652320845	32.48413775839327	28.91037953843043
3	21.18487815680271	19.07669237360754	27.576290401779268	32.16213906781048
4	15.461533462349442	14.061913289621906	31.08126811280244	39.39528513522621
5	17.488903651926094	33.80721479256328	31.10232951099044	17.601552044520187
6	38.326028036933266	30.681462599770853	15.386675235646956	15.60583412764892
7	33.79758672482019	27.405452374762906	18.163771025292935	20.63318987512396
8	31.15648739204529	30.35844092699036	17.551907320219907	20.933164360744442
9	29.333713642009187	13.414485909322856	16.25807554181951	40.99372490684844
10	20.133553334681263	23.351193398996756	28.309588111261952	28.205665155060032
11	39.82764555231411	20.090046503567198	18.120865948412813	21.961441995705883
12	26.865558476069438	22.199014567266495	24.73974129381974	26.195685662844326
13	32.683198058981546	16.062084187824347	23.08503750132386	28.169680251870254
14	25.018473854982044	17.679298691545593	21.698415220049487	35.60381223342288
15	29.28467067194285	23.143588188286493	19.21714181180979	28.354599327960873
16	28.768365539219936	22.472933094557256	19.890143747051404	28.86855761917141
17	27.069613336799435	23.51156090234251	21.91318130614367	27.505644454714385
18	27.740689658492435	20.433768521995322	23.468054071228444	28.3574877482838
19	28.142781837613008	22.212975265493967	21.630296640767163	28.01394625612586
20	28.02664327046205	21.321416192484328	22.28614858034141	28.365791956712204
21	31.66358568498888	20.787900888670652	21.20443516940585	26.344078256934615
22	30.66527541087779	21.219960428641578	20.428773961853597	27.685990198627035
23	28.486864908581495	21.384419860778138	21.111644666531873	29.017070564108487
24	28.402679491252904	21.621872081491965	21.91348218326064	28.06196624399449
25	27.895942250849675	20.83074579012738	22.002180757343808	29.271131201679136
26	29.476269220030233	21.537445962469793	22.19594562067339	26.79033919682659
27	30.059128371027217	20.400190635741314	21.233018495518134	28.30766249771333
28	27.625453722692395	20.50369236397947	22.789094769070793	29.08175914425734
29	27.079421930812703	20.720745116162636	23.904807294224124	28.295025658800537
30	26.147124096165143	21.95957655758066	23.96877376929224	27.924525576961962
31	27.500589719149264	21.868410791138327	22.142148792158903	28.48885069755351
32	26.489522255278587	21.1523834281698	23.593880881545886	28.764213435005733
33	27.710722297642086	20.87930735680656	23.17054677796713	28.239423567584222
34	28.447991585068795	21.472817557744335	22.749740042170938	27.32945081501593
35	28.19832375340593	20.86534665857909	23.772361187333313	27.163968400681664
36	26.731848685287353	21.839767289602648	22.72025408470774	28.70812994040226
37	26.481398573120362	20.632106717502865	24.287522987011737	28.59897172236504
38	26.592963808093355	19.469878590065758	25.433443574708992	28.503714027131892
39	25.885661881516995	19.560322251427362	26.057703417001242	28.4963124500544
40	25.64905211673069	18.84730365962855	26.993190549088702	28.510453674552057
41	23.519383707383763	19.349046339890048	28.55985769715876	28.571712255567427
42	24.084250406785863	19.812397100025997	27.80399420390322	28.299358289284925
43	22.777721614049277	18.273169945023735	29.16040842263366	29.78870001829333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	11.5
2	15.0
3	78.0
4	141.0
5	141.0
6	209.5
7	278.0
8	288.5
9	299.0
10	415.0
11	531.0
12	531.0
13	954.5
14	1378.0
15	2430.0
16	3482.0
17	3183.5
18	2885.0
19	2885.0
20	3236.0
21	3587.0
22	2945.5
23	2304.0
24	2718.0
25	3132.0
26	3132.0
27	4022.5
28	4913.0
29	6570.5
30	8228.0
31	10716.0
32	13204.0
33	13204.0
34	17579.0
35	21954.0
36	28214.5
37	34475.0
38	41453.5
39	48432.0
40	48432.0
41	54102.5
42	59773.0
43	67324.0
44	74875.0
45	81852.0
46	88829.0
47	88829.0
48	100634.0
49	112439.0
50	120736.5
51	129034.0
52	137232.0
53	145430.0
54	145430.0
55	145414.5
56	145399.0
57	139214.5
58	133030.0
59	125832.0
60	118634.0
61	118634.0
62	116441.5
63	114249.0
64	106692.5
65	99136.0
66	88355.0
67	77574.0
68	77574.0
69	66444.5
70	55315.0
71	50113.0
72	44911.0
73	43516.0
74	42121.0
75	42121.0
76	39716.5
77	37312.0
78	29137.5
79	20963.0
80	13822.0
81	6681.0
82	6681.0
83	4927.0
84	3173.0
85	2522.5
86	1872.0
87	1443.5
88	1015.0
89	1015.0
90	798.0
91	581.0
92	361.5
93	142.0
94	93.5
95	45.0
96	45.0
97	32.0
98	19.0
99	14.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1661808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.98524929073166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30573139824203	40.147758228578176
2	6.727384181410565	6.187208773133792
3	1.985078542575696	2.7385299492607698
4	0.930106673456841	1.7108474898354393
5	0.5680186601093732	1.306023984345845
6	0.3769787248757	1.0401276384426728
7	0.25864569518388514	0.8325720739703885
8	0.2043360351881552	0.7517154813765627
9	0.15537762909857422	0.64305711074707
>10	1.1412762239364913	10.551716612276635
>50	0.1640816592732616	5.293076865872699
>100	0.1512585214429872	14.43137950274381
>500	0.02343252210277755	7.651818360641584
>1k	0.008030246338592307	5.940147758211673
>5k	2.6328676519974775E-4	0.7740201705630007
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	7718	0.4644339177570453	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	5068	0.304969045762206	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3244	0.19520907349104108	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2932	0.17643434139202602	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2905	0.1748096049603805	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2900	0.1745087278434091	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2612	0.15717820590585677	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2533	0.1524243474577087	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2495	0.1501376813687261	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2385	0.14351838479535542	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2375	0.14291663056141263	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2236	0.13455224670960783	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2206	0.13274698400777948	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2134	0.1284143535233914	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2039	0.12269768830093489	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1989	0.11968891713122094	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1841	0.11078295446886764	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1830	0.11012102481153056	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1829	0.1100608493881363	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1698	0.10217786892348574	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1685	0.10139558841936011	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1684	0.10133541299596584	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1667	0.10031243079826309	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1666	0.10025225537486883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.0175423394279E-5	0.0
10	0.0	0.0	0.0	1.20350846788558E-4	0.0
11	0.0	0.0	0.0	1.20350846788558E-4	0.0
12	0.0	0.0	0.0	1.20350846788558E-4	0.0
13	0.0	0.0	0.0	1.80526270182837E-4	0.0
14	0.0	0.0	0.0	3.00877116971395E-4	0.0
15	0.0	0.0	0.0	3.00877116971395E-4	6.0175423394279E-5
16	0.0	0.0	0.0	8.42455927519906E-4	1.80526270182837E-4
17	0.0	0.0	0.0	0.0011433330444913011	1.80526270182837E-4
18	0.0	0.0	0.0	0.00120350846788558	1.80526270182837E-4
19	0.0	0.0	0.0	0.001564561008251254	1.80526270182837E-4
20	0.0	0.0	0.0	0.0018052627018283701	1.80526270182837E-4
21	0.0	0.0	0.0	0.002768069476136834	1.80526270182837E-4
22	0.0	0.0	0.0	0.003791051673839577	1.80526270182837E-4
23	0.0	0.0	0.0	0.005716665222456506	1.80526270182837E-4
24	0.0	0.0	0.0	0.008905962662353292	1.80526270182837E-4
25	0.0	0.0	0.0	0.010530699093998825	1.80526270182837E-4
26	0.0	0.0	0.0	0.01570578550590682	1.80526270182837E-4
27	0.0	0.0	0.0	0.043567006537458	1.80526270182837E-4
28	0.0	6.0175423394279E-5	0.0	0.18365539219933952	1.80526270182837E-4
29	0.0	1.20350846788558E-4	0.0	0.4034762138586407	1.80526270182837E-4
30	0.0	1.20350846788558E-4	0.0	0.6949057893571339	1.80526270182837E-4
31	0.0	1.20350846788558E-4	0.0	1.4103915735151114	1.80526270182837E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATCA	1660	0.0	24.183735	14
GACAGGC	1695	0.0	24.120945	7
GCATCAG	1705	0.0	23.21994	15
CAGGACA	2000	0.0	22.57	4
GGTATCA	2010	0.0	22.549753	1
ACAGGCT	1835	0.0	22.179836	8
GCTGCAT	1795	0.0	21.952644	12
CTGCATC	1860	0.0	21.682796	13
CCAGGAC	2075	0.0	21.575903	3
TCCAGGA	2095	0.0	21.458233	2
CATCAGA	1965	0.0	20.24173	16
CAGAAGA	2015	0.0	20.1067	19
ATCAGAA	1990	0.0	20.080402	17
AGAAGAG	2045	0.0	19.992666	20
GGACAGG	2210	0.0	19.923077	6
AAGAGGC	2080	0.0	19.478365	22
TCTAGAT	295	0.0	19.440678	2
GGCCATC	2090	0.0	19.119617	26
CCATCAA	2080	0.0	19.033653	28
TCAGAAG	2140	0.0	19.018692	18
>>END_MODULE