FastQCFastQC Report
Fri 10 Feb 2017
ERR1631420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531759
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11230.21118589436191962No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10130.1904998316906719No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC9000.16924960367384473No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7270.1367160687454279No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7050.13257885621117838No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6290.11828666745649814No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT6010.11302112423108965No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT5990.11264501400070333No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC5910.11114057307915805No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG5840.1098241872728059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTA358.863101E-426.42857220
TTACTAT358.863101E-426.42857221
GTATAGT451.3219059E-424.6666681
CGAACGA1002.0008883E-1124.05000116
GAATCTA551.9001665E-523.54545413
AATCTAC551.9001665E-523.54545414
TACTATG400.001929829723.12500222
TAACGAA1054.0017767E-1122.90476413
ACGAACG1054.0017767E-1122.90476415
ATAACGA1107.4578566E-1121.86363612
GGGTAGA603.72144E-521.5833341
AACGAGA1201.0913936E-1121.58333418
TATAGTA603.72144E-521.5833342
TAGAACT1550.021.483874
ACTGATC1059.786163E-1021.1428598
CAACTAA705.0934177E-621.14285926
CTAGAAC1500.020.9666673
TGGATTA1251.8189894E-1120.7219
ACCATGA1700.020.6764728
TTACTCC450.003823144620.5555574
Produced by FastQC (version 0.11.5)