##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	531759
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.899727132027856	34.0	33.0	34.0	31.0	34.0
2	33.07079898976792	34.0	33.0	34.0	31.0	34.0
3	33.183246545897674	34.0	33.0	34.0	31.0	34.0
4	36.49525630971925	37.0	37.0	37.0	35.0	37.0
5	36.46922195957191	37.0	37.0	37.0	35.0	37.0
6	36.51028567452549	37.0	37.0	37.0	35.0	37.0
7	36.497157546933856	37.0	37.0	37.0	35.0	37.0
8	36.47202021968599	37.0	37.0	37.0	35.0	37.0
9	38.34222834028197	39.0	39.0	39.0	37.0	39.0
10	38.295648968799775	39.0	39.0	39.0	37.0	39.0
11	38.36645171967	39.0	39.0	39.0	37.0	39.0
12	38.144131081937495	39.0	39.0	39.0	37.0	39.0
13	38.26840166315944	39.0	39.0	39.0	37.0	39.0
14	39.824480638785616	41.0	40.0	41.0	38.0	41.0
15	39.829575051856196	41.0	40.0	41.0	38.0	41.0
16	39.83313869628911	41.0	40.0	41.0	38.0	41.0
17	39.80911089422088	41.0	40.0	41.0	38.0	41.0
18	39.79279335187557	41.0	40.0	41.0	38.0	41.0
19	39.791990356533695	41.0	40.0	41.0	38.0	41.0
20	39.76518874151636	41.0	40.0	41.0	38.0	41.0
21	39.727395305015996	41.0	40.0	41.0	38.0	41.0
22	39.687954505706536	41.0	40.0	41.0	37.0	41.0
23	39.6376365985343	41.0	40.0	41.0	37.0	41.0
24	39.611252465872695	41.0	40.0	41.0	37.0	41.0
25	39.579890514311934	41.0	40.0	41.0	37.0	41.0
26	39.53127262538105	41.0	39.0	41.0	37.0	41.0
27	39.45568951348261	41.0	39.0	41.0	37.0	41.0
28	39.37808480909585	41.0	39.0	41.0	36.0	41.0
29	39.31678072209403	41.0	39.0	41.0	36.0	41.0
30	39.26129506035629	41.0	39.0	41.0	36.0	41.0
31	39.23157671050232	41.0	39.0	41.0	36.0	41.0
32	39.15291889747047	41.0	39.0	41.0	35.0	41.0
33	39.06180055250593	40.0	39.0	41.0	35.0	41.0
34	39.01474728213345	40.0	39.0	41.0	35.0	41.0
35	38.931826259640175	40.0	39.0	41.0	35.0	41.0
36	38.86340616707945	40.0	39.0	41.0	35.0	41.0
37	38.80643486993168	40.0	38.0	41.0	35.0	41.0
38	38.70198341729994	40.0	38.0	41.0	35.0	41.0
39	38.6167060642133	40.0	38.0	41.0	35.0	41.0
40	38.534275865570685	40.0	38.0	41.0	35.0	41.0
41	38.444216270904676	40.0	38.0	41.0	35.0	41.0
42	38.321758540993194	40.0	37.0	41.0	34.0	41.0
43	37.3379745335763	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	1.0
15	1.0
16	1.0
17	0.0
18	6.0
19	7.0
20	33.0
21	67.0
22	101.0
23	171.0
24	304.0
25	458.0
26	677.0
27	957.0
28	1415.0
29	1915.0
30	2694.0
31	3575.0
32	4844.0
33	6533.0
34	10044.0
35	14822.0
36	25000.0
37	46888.0
38	115350.0
39	295892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96397804268475	16.08717482919894	10.479371294138886	28.46947583397742
2	21.475329989713384	18.736119181809805	30.409828512540454	29.378722315936358
3	21.437718966674755	19.244620213292112	27.338136260975364	31.979524559057772
4	15.768609464061727	13.648476095374033	30.969104425124915	39.61381001543933
5	17.78926167681224	33.22538969721246	31.49490652720499	17.490442098770306
6	39.56397540991314	30.861348844119235	13.905547437843083	15.669128308124543
7	33.26300072025109	26.904481165339938	18.64735716743863	21.18516094697034
8	30.31429651402233	29.2730353411978	18.234388134474454	22.17828001030542
9	28.05895151751075	12.523342341173352	16.99679742138826	42.42090871992764
10	18.67048794660739	24.032315390994793	30.177580445276902	27.119616217120914
11	41.14965614122187	18.765643834895133	18.085072373011084	21.99962765087192
12	25.296421875323222	22.287351977117453	25.445737636786593	26.97048851077274
13	33.442029189914976	16.285008810382145	22.247108182466118	28.025853817236758
14	25.324818197717384	17.672855560507674	20.554612145727667	36.44771409604727
15	28.621048256823112	24.499632352249797	19.153037372193044	27.72628201873405
16	29.541013880348054	22.176211404038295	20.044606673323816	28.238168042289836
17	27.661779114222796	23.06608820913233	21.19305926180845	28.07907341483642
18	28.540372612405246	20.676283805257643	22.771217788509457	28.012125793827654
19	28.22293557795919	21.795963961117724	21.31191009461053	28.66919036631256
20	28.029426864425428	21.4804074778236	22.178468065420613	28.311697592330358
21	29.833627639588613	21.55788618528318	21.929859203135255	26.67862697199295
22	29.268522018433163	21.269221583461682	20.798143519902812	28.664112878202342
23	27.921859338534937	21.026630484862505	22.071088594645317	28.980421581957238
24	27.694312649151215	21.83075415742846	21.320748685024608	29.15418450839572
25	28.612961886869805	21.492819115426347	21.62558602675272	28.268632970951124
26	29.031572573289782	21.666770096980024	21.113700003196936	28.187957326533258
27	29.05413918711296	21.042051004308345	21.790134252546736	28.113675556031964
28	27.487640077553934	21.58402584629503	22.04795781547656	28.880376260674478
29	27.167194161264785	21.70945860812887	22.28471920550475	28.8386280251016
30	26.488691305647862	22.35317126743506	23.318458173721552	27.839679253195527
31	28.847090505285287	21.98006991889183	21.08530368080277	28.08753589502011
32	27.558348800866554	21.623705475600786	21.869305456043055	28.948640267489594
33	27.533525525661062	21.250980237287944	22.112460719987816	29.10303351706318
34	28.205258397131033	21.061232626058047	22.204419671317268	28.529089305493656
35	27.732299782420235	21.302695393966065	22.95927290370262	28.005731919911085
36	26.904857275570325	22.078234689022658	23.099185909406327	27.917722126000687
37	28.35288166255766	20.922071840815107	23.04521409134589	27.679832405281342
38	26.958452983400377	20.635663900375924	24.1118627047215	28.294020411502203
39	27.785519380019895	20.68907155309078	23.843320000225667	27.682089066663657
40	27.086894627077303	21.56954560242516	23.46946643122166	27.874093339275873
41	26.55582698177182	20.76354137870727	25.427684345728046	27.252947293792868
42	27.290558316831497	20.639236947564594	24.962624045855357	27.107580689748552
43	25.099152059485597	20.297540803258617	25.076021280316834	29.52728585693895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	11.0
4	19.0
5	19.0
6	19.0
7	19.0
8	16.5
9	14.0
10	20.0
11	26.0
12	26.0
13	50.0
14	74.0
15	126.0
16	178.0
17	176.0
18	174.0
19	174.0
20	228.5
21	283.0
22	291.0
23	299.0
24	373.0
25	447.0
26	447.0
27	640.5
28	834.0
29	1226.0
30	1618.0
31	2127.0
32	2636.0
33	2636.0
34	3844.5
35	5053.0
36	6995.5
37	8938.0
38	11148.5
39	13359.0
40	13359.0
41	15237.0
42	17115.0
43	19215.5
44	21316.0
45	25736.5
46	30157.0
47	30157.0
48	33462.5
49	36768.0
50	39690.5
51	42613.0
52	45587.5
53	48562.0
54	48562.0
55	48608.5
56	48655.0
57	47960.5
58	47266.0
59	47115.5
60	46965.0
61	46965.0
62	44660.5
63	42356.0
64	39544.5
65	36733.0
66	33745.0
67	30757.0
68	30757.0
69	24752.5
70	18748.0
71	16102.5
72	13457.0
73	9894.0
74	6331.0
75	6331.0
76	4892.0
77	3453.0
78	2889.5
79	2326.0
80	2065.0
81	1804.0
82	1804.0
83	1391.0
84	978.0
85	804.0
86	630.0
87	546.5
88	463.0
89	463.0
90	359.5
91	256.0
92	150.0
93	44.0
94	31.5
95	19.0
96	19.0
97	13.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	531759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.92240645842368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87188949805613	38.51598999965729
2	7.5207547590521635	6.907423138386354
3	2.6417572466090533	3.6394755012980147
4	1.351160274113316	2.4819412519322737
5	0.8134710269277433	1.867827357036357
6	0.5459478196255693	1.5042742606761341
7	0.4101308023786515	1.3183935385566308
8	0.30858208283220906	1.1336665466886136
9	0.24042307723909137	0.993672564746269
>10	1.8427752271731606	17.422355214715928
>50	0.2781090135279222	8.846624103034042
>100	0.1708912106700362	13.958782468551261
>500	0.0032863694359622346	1.0066230133820246
>1k	8.215923589905586E-4	0.4029510413388315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1123	0.21118589436191962	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1013	0.1904998316906719	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	900	0.16924960367384473	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	727	0.1367160687454279	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	705	0.13257885621117838	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	629	0.11828666745649814	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	601	0.11302112423108965	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	599	0.11264501400070333	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	591	0.11114057307915805	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	584	0.1098241872728059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8805511519316082E-4	0.0	0.0	0.0
3	0.0	1.8805511519316082E-4	0.0	0.0	0.0
4	0.0	1.8805511519316082E-4	0.0	0.0	0.0
5	0.0	1.8805511519316082E-4	0.0	0.0	0.0
6	0.0	1.8805511519316082E-4	0.0	0.0	0.0
7	0.0	1.8805511519316082E-4	0.0	0.0	0.0
8	0.0	1.8805511519316082E-4	0.0	0.0	0.0
9	0.0	1.8805511519316082E-4	0.0	0.0	0.0
10	0.0	1.8805511519316082E-4	0.0	0.0	0.0
11	0.0	3.7611023038632165E-4	0.0	0.0	0.0
12	0.0	3.7611023038632165E-4	0.0	0.0	0.0
13	0.0	3.7611023038632165E-4	0.0	1.8805511519316082E-4	0.0
14	0.0	3.7611023038632165E-4	0.0	3.7611023038632165E-4	0.0
15	0.0	3.7611023038632165E-4	0.0	7.522204607726433E-4	0.0
16	0.0	3.7611023038632165E-4	0.0	7.522204607726433E-4	0.0
17	0.0	3.7611023038632165E-4	0.0	7.522204607726433E-4	0.0
18	0.0	3.7611023038632165E-4	0.0	0.0011283306911589648	0.0
19	0.0	3.7611023038632165E-4	0.0	0.0011283306911589648	0.0
20	0.0	3.7611023038632165E-4	0.0	0.0011283306911589648	0.0
21	0.0	3.7611023038632165E-4	0.0	0.0011283306911589648	0.0
22	0.0	3.7611023038632165E-4	0.0	0.0013163858063521257	0.0
23	0.0	3.7611023038632165E-4	0.0	0.002068606267124769	0.0
24	1.8805511519316082E-4	3.7611023038632165E-4	0.0	0.0033849920734768946	0.0
25	1.8805511519316082E-4	3.7611023038632165E-4	0.0	0.0033849920734768946	0.0
26	1.8805511519316082E-4	3.7611023038632165E-4	0.0	0.005077488110215342	0.0
27	1.8805511519316082E-4	3.7611023038632165E-4	0.0	0.012787747833134935	0.0
28	1.8805511519316082E-4	3.7611023038632165E-4	0.0	0.052843487369278186	0.0
29	1.8805511519316082E-4	5.641653455794824E-4	0.0	0.10831974635126063	0.0
30	1.8805511519316082E-4	5.641653455794824E-4	0.0	0.19708176072243253	0.0
31	1.8805511519316082E-4	5.641653455794824E-4	0.0	0.4300820484467588	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACTA	35	8.863101E-4	26.428572	20
TTACTAT	35	8.863101E-4	26.428572	21
GTATAGT	45	1.3219059E-4	24.666668	1
CGAACGA	100	2.0008883E-11	24.050001	16
GAATCTA	55	1.9001665E-5	23.545454	13
AATCTAC	55	1.9001665E-5	23.545454	14
TACTATG	40	0.0019298297	23.125002	22
TAACGAA	105	4.0017767E-11	22.904764	13
ACGAACG	105	4.0017767E-11	22.904764	15
ATAACGA	110	7.4578566E-11	21.863636	12
GGGTAGA	60	3.72144E-5	21.583334	1
AACGAGA	120	1.0913936E-11	21.583334	18
TATAGTA	60	3.72144E-5	21.583334	2
TAGAACT	155	0.0	21.48387	4
ACTGATC	105	9.786163E-10	21.142859	8
CAACTAA	70	5.0934177E-6	21.142859	26
CTAGAAC	150	0.0	20.966667	3
TGGATTA	125	1.8189894E-11	20.72	19
ACCATGA	170	0.0	20.676472	8
TTACTCC	45	0.0038231446	20.555557	4
>>END_MODULE