FastQCFastQC Report
Fri 10 Feb 2017
ERR1631419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412146
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.16935746070567226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6190.1501894959553168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5040.12228676245796392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATAC405.931028E-527.7537
CGCAATA405.931028E-527.7536
TCTAGCG502.6980287E-422.19999928
GAGCCGC1059.767973E-1021.14285916
GGTATCA6600.020.7424241
GACAATC450.003821707320.55555536
AGCTATG450.003821707320.5555557
TACCGCA1001.2838427E-820.34999828
GTTCAAA1153.0340743E-919.3043481
GCAGTCG2700.019.1851869
CGCCTTA2550.018.86274525
CAGTCGG2850.018.8245610
CGGTGAT2950.018.81355914
CCCCTAT609.2231174E-418.51
GCGCAAT609.2231174E-418.535
AACGAAC609.2231174E-418.514
CCTATAC609.2231174E-418.53
AGTCGGT2900.018.511
CTAGCGG609.2231174E-418.529
TAACGCC500.00702741418.4999984
Produced by FastQC (version 0.11.5)