##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631419.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412146
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.862531724194824	34.0	33.0	34.0	31.0	34.0
2	33.03368223881828	34.0	33.0	34.0	31.0	34.0
3	33.148743406462756	34.0	33.0	34.0	31.0	34.0
4	36.474467785687594	37.0	37.0	37.0	35.0	37.0
5	36.45163607071281	37.0	37.0	37.0	35.0	37.0
6	36.501492189660944	37.0	37.0	37.0	35.0	37.0
7	36.490673208037926	37.0	37.0	37.0	35.0	37.0
8	36.47421302159914	37.0	37.0	37.0	35.0	37.0
9	38.32461797518355	39.0	39.0	39.0	37.0	39.0
10	38.2906664143289	39.0	39.0	39.0	37.0	39.0
11	38.35431376259869	39.0	39.0	39.0	37.0	39.0
12	38.13279760085018	39.0	39.0	39.0	37.0	39.0
13	38.26314218747725	39.0	39.0	39.0	37.0	39.0
14	39.79260504772581	41.0	40.0	41.0	38.0	41.0
15	39.804035463161114	41.0	40.0	41.0	38.0	41.0
16	39.776884890305865	41.0	40.0	41.0	38.0	41.0
17	39.767097096659924	41.0	40.0	41.0	38.0	41.0
18	39.76753140877262	41.0	40.0	41.0	38.0	41.0
19	39.779459220761574	41.0	40.0	41.0	38.0	41.0
20	39.76674285326074	41.0	40.0	41.0	38.0	41.0
21	39.741795383189455	41.0	40.0	41.0	38.0	41.0
22	39.69074308618791	41.0	40.0	41.0	37.0	41.0
23	39.635583021550616	41.0	40.0	41.0	37.0	41.0
24	39.61195789841464	41.0	40.0	41.0	37.0	41.0
25	39.58492864179198	41.0	40.0	41.0	37.0	41.0
26	39.52704624089522	41.0	39.0	41.0	37.0	41.0
27	39.455705017154116	41.0	39.0	41.0	37.0	41.0
28	39.38003523023394	41.0	39.0	41.0	36.0	41.0
29	39.31722496396908	41.0	39.0	41.0	36.0	41.0
30	39.262909260310664	41.0	39.0	41.0	36.0	41.0
31	39.20647780155576	41.0	39.0	41.0	35.0	41.0
32	39.13240938890587	41.0	39.0	41.0	35.0	41.0
33	39.043797586292236	40.0	39.0	41.0	35.0	41.0
34	39.002736894207395	40.0	39.0	41.0	35.0	41.0
35	38.91383150631087	40.0	39.0	41.0	35.0	41.0
36	38.86455285262989	40.0	39.0	41.0	35.0	41.0
37	38.80687668932854	40.0	38.0	41.0	35.0	41.0
38	38.72789739558312	40.0	38.0	41.0	35.0	41.0
39	38.63973446303009	40.0	38.0	41.0	35.0	41.0
40	38.56183003110548	40.0	38.0	41.0	35.0	41.0
41	38.47171875985694	40.0	38.0	41.0	35.0	41.0
42	38.37672087075939	40.0	38.0	41.0	35.0	41.0
43	37.47280575330102	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	2.0
18	4.0
19	15.0
20	27.0
21	52.0
22	76.0
23	157.0
24	232.0
25	365.0
26	519.0
27	788.0
28	1049.0
29	1516.0
30	2131.0
31	2854.0
32	3841.0
33	5251.0
34	7823.0
35	11941.0
36	19126.0
37	36013.0
38	87353.0
39	231008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34472250124956	16.795261873219687	12.056407195508388	26.80360843002237
2	21.005420409272443	18.97604247038671	32.08134981293037	27.937187307410483
3	21.21942224357388	20.358076992133856	28.05462142056456	30.36787934372771
4	15.686188874816207	14.76928078884667	32.465437005333065	37.07909333100406
5	17.087148728848515	33.92923866785071	32.126479451456525	16.857133151844252
6	36.61032740824853	32.04277125096446	15.29021269161899	16.056688649168013
7	31.7050268594139	28.310841303809813	19.2608929845249	20.723238852251384
8	30.013878577009116	29.717381704541594	18.761798003620076	21.50694171482921
9	27.842318013519478	13.609254972752375	18.39809193829371	40.150335075434434
10	19.605188452635716	24.07763268356359	30.22933620610172	26.087842657698975
11	37.61846530113115	20.70576931475739	19.10924769377842	22.566517690333036
12	24.48088784071664	24.136592372605826	26.029125601121933	25.353394185555604
13	33.00383844559938	17.53092350768902	22.656776967385344	26.808461079326257
14	24.790486866304658	19.311845802215718	22.427731920241854	33.46993541123776
15	28.39260844458032	24.89748778345538	20.07080015334372	26.639103618620585
16	27.34176723782349	23.818015945805612	22.025447292949586	26.81476952342131
17	25.86462078972015	24.254269118225093	22.956670694365588	26.92443939768917
18	26.194843574849692	22.210575863892892	24.546641238784314	27.047939322473102
19	26.763088808334913	23.562281327490744	23.916767359139723	25.757862505034623
20	27.76055087274898	22.30301883313195	23.384674362968465	26.551755931150613
21	28.36834519806088	22.46849417439451	23.094485934595994	26.06867469294862
22	28.509799925269203	22.423849800798745	22.636881105239407	26.42946916869265
23	27.198856715824004	22.524299641389216	22.67764335939206	27.59920028339472
24	26.420977032410846	23.253652831763503	23.669524877106657	26.655845258718998
25	26.7939031314146	22.88630727945922	22.889461501506748	27.430328087619436
26	27.07705521829643	22.949634352874952	23.04426101430076	26.92904941452786
27	27.051093544520633	22.275601364564984	23.343184211420226	27.330120879494157
28	26.315917174981678	22.746308347042067	23.647202690308774	27.290571787667478
29	26.04610987368554	23.24734438766845	24.416832869905324	26.28971286874069
30	26.061395718992785	23.457464102526774	24.007269268657225	26.473870909823216
31	26.465621406006612	23.67801701338846	23.349492655515277	26.506868925089655
32	25.499701562067813	22.95958228394792	24.2627612545069	27.27795489947737
33	25.31724194824164	22.857191383635897	24.80043479737763	27.025131870744833
34	26.85625967496955	21.73889835155503	24.268584433671563	27.136257539803854
35	25.724136592372602	21.980075021958235	25.065632081835076	27.23015630383408
36	24.750209877082394	22.76304998714048	25.28764078748793	27.199099348289195
37	25.366253706210905	21.260184497726534	26.033250353030233	27.340311443032324
38	24.734924031775147	20.85498828085193	26.784925730202403	27.62516195717052
39	24.492291566580775	20.31780000291159	28.05462142056456	27.135287009943077
40	23.52443066292042	20.213710675343204	28.458847107578382	27.80301155415799
41	22.38017595706376	20.543448195542357	29.756930796368273	27.31944505102561
42	22.463884157555817	20.46604843914535	29.913914001349035	27.156153401949794
43	21.625588990309257	20.541021870890415	29.831661595648146	28.00172754315218
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	13.5
2	10.0
3	32.0
4	54.0
5	54.0
6	88.5
7	123.0
8	118.0
9	113.0
10	196.0
11	279.0
12	279.0
13	480.0
14	681.0
15	1079.5
16	1478.0
17	1332.0
18	1186.0
19	1186.0
20	1445.5
21	1705.0
22	1580.5
23	1456.0
24	1809.0
25	2162.0
26	2162.0
27	2675.0
28	3188.0
29	4068.0
30	4948.0
31	5814.5
32	6681.0
33	6681.0
34	7971.0
35	9261.0
36	10736.5
37	12212.0
38	13608.0
39	15004.0
40	15004.0
41	16349.5
42	17695.0
43	19119.5
44	20544.0
45	22546.5
46	24549.0
47	24549.0
48	27330.5
49	30112.0
50	30462.5
51	30813.0
52	31479.5
53	32146.0
54	32146.0
55	31477.0
56	30808.0
57	30728.0
58	30648.0
59	30378.0
60	30108.0
61	30108.0
62	28252.0
63	26396.0
64	24684.0
65	22972.0
66	20445.5
67	17919.0
68	17919.0
69	15774.5
70	13630.0
71	11617.0
72	9604.0
73	7307.5
74	5011.0
75	5011.0
76	4136.0
77	3261.0
78	2680.0
79	2099.0
80	1787.0
81	1475.0
82	1475.0
83	1183.0
84	891.0
85	659.0
86	427.0
87	350.0
88	273.0
89	273.0
90	210.5
91	148.0
92	92.0
93	36.0
94	23.0
95	10.0
96	10.0
97	10.0
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	412146.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.08615931889021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.09875087117183	67.31143979241243
2	4.4303254115125	6.475909377206608
3	1.1402440667998914	2.500081785856684
4	0.5653131024424164	1.6526625388065017
5	0.32683441187482226	1.1943535948589519
6	0.23592739212356353	1.034581618105983
7	0.17047118057878596	0.8721358704142318
8	0.1436568081838136	0.8399459496132362
9	0.1052048854910651	0.6920118919913028
>10	0.6736761676367325	9.62608325262089
>50	0.07728329154011854	3.806298760654679
>100	0.031313057737971724	3.5511496524013983
>500	9.993529065310124E-4	0.4433459150571059
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.16935746070567226	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.1501894959553168	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	504	0.12228676245796392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	2.4263246519437288E-4	0.0	0.0	0.0	0.0
11	2.4263246519437288E-4	0.0	0.0	0.0	0.0
12	2.4263246519437288E-4	0.0	0.0	0.0	0.0
13	2.4263246519437288E-4	0.0	0.0	0.0	0.0
14	2.4263246519437288E-4	0.0	0.0	0.0	2.4263246519437288E-4
15	2.4263246519437288E-4	0.0	0.0	0.0	2.4263246519437288E-4
16	2.4263246519437288E-4	0.0	0.0	2.4263246519437288E-4	2.4263246519437288E-4
17	2.4263246519437288E-4	0.0	0.0	7.278973955831186E-4	2.4263246519437288E-4
18	2.4263246519437288E-4	0.0	0.0	9.705298607774915E-4	2.4263246519437288E-4
19	2.4263246519437288E-4	0.0	0.0	0.0014557947911662372	2.4263246519437288E-4
20	2.4263246519437288E-4	0.0	0.0	0.001941059721554983	2.4263246519437288E-4
21	2.4263246519437288E-4	0.0	0.0	0.001941059721554983	2.4263246519437288E-4
22	2.4263246519437288E-4	0.0	0.0	0.003882119443109966	2.4263246519437288E-4
23	2.4263246519437288E-4	0.0	0.0	0.007036341490636813	2.4263246519437288E-4
24	2.4263246519437288E-4	0.0	0.0	0.009220033677386169	2.4263246519437288E-4
25	2.4263246519437288E-4	0.0	0.0	0.013344785585690508	2.4263246519437288E-4
26	2.4263246519437288E-4	0.0	0.0	0.02207955433268793	2.4263246519437288E-4
27	2.4263246519437288E-4	0.0	0.0	0.06478286820689756	2.4263246519437288E-4
28	7.278973955831186E-4	0.0	0.0	0.20599496295002256	2.4263246519437288E-4
29	7.278973955831186E-4	0.0	0.0	0.415144147947572	2.4263246519437288E-4
30	7.278973955831186E-4	0.0	0.0	0.6868925089652695	2.4263246519437288E-4
31	7.278973955831186E-4	0.0	0.0	1.400959854032309	2.4263246519437288E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAATAC	40	5.931028E-5	27.75	37
CGCAATA	40	5.931028E-5	27.75	36
TCTAGCG	50	2.6980287E-4	22.199999	28
GAGCCGC	105	9.767973E-10	21.142859	16
GGTATCA	660	0.0	20.742424	1
GACAATC	45	0.0038217073	20.555555	36
AGCTATG	45	0.0038217073	20.555555	7
TACCGCA	100	1.2838427E-8	20.349998	28
GTTCAAA	115	3.0340743E-9	19.304348	1
GCAGTCG	270	0.0	19.185186	9
CGCCTTA	255	0.0	18.862745	25
CAGTCGG	285	0.0	18.82456	10
CGGTGAT	295	0.0	18.813559	14
CCCCTAT	60	9.2231174E-4	18.5	1
GCGCAAT	60	9.2231174E-4	18.5	35
AACGAAC	60	9.2231174E-4	18.5	14
CCTATAC	60	9.2231174E-4	18.5	3
AGTCGGT	290	0.0	18.5	11
CTAGCGG	60	9.2231174E-4	18.5	29
TAACGCC	50	0.007027414	18.499998	4
>>END_MODULE