FastQCFastQC Report
Fri 10 Feb 2017
ERR1631418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773439
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.509154568104272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31060.40158305955608653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28120.36357101206429987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.13071489800747055No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT8490.10976948408342481No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8430.10899372801216387No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8390.10847655729798988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT400.001930618923.1250024
CTCCGAT400.001930618923.12500214
TTATGCG652.680963E-622.769234
ATGCGGA753.7374957E-722.26
TTCGCCA502.700995E-422.219
CTTATGC705.09836E-621.1428593
TATGCGG806.954051E-720.81255
GTATTGA450.003824693420.5555551
AGCGTCA851.2442306E-619.5882363
GCAGTCG4600.018.9021749
CTATAGA500.007032852618.51
AAGACGG2300.018.55
AATATAG609.233093E-418.55
ATATAGC609.233093E-418.56
CCGTTAT500.007032852618.59
GGTATCA15050.018.438541
AGTCGGT4850.018.30927811
AGACGGA2650.018.1509446
GACGGAC2450.018.1224487
CAGTCGG5150.017.96116610
Produced by FastQC (version 0.11.5)