##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773439
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05881394654265	34.0	33.0	34.0	31.0	34.0
2	33.18428861228875	34.0	33.0	34.0	31.0	34.0
3	33.267506551906486	34.0	34.0	34.0	31.0	34.0
4	36.49942400111709	37.0	37.0	37.0	35.0	37.0
5	36.49065795751184	37.0	37.0	37.0	35.0	37.0
6	36.54435062105738	37.0	37.0	37.0	35.0	37.0
7	36.54723901949604	37.0	37.0	37.0	35.0	37.0
8	36.54941242942236	37.0	37.0	37.0	35.0	37.0
9	38.397728844808704	39.0	39.0	39.0	37.0	39.0
10	38.34721419530176	39.0	39.0	39.0	37.0	39.0
11	38.40527695138207	39.0	39.0	39.0	37.0	39.0
12	38.368235891906146	39.0	39.0	39.0	37.0	39.0
13	38.397383633356995	39.0	39.0	39.0	37.0	39.0
14	39.93087366941672	41.0	40.0	41.0	38.0	41.0
15	39.917590139623165	41.0	40.0	41.0	38.0	41.0
16	39.87920055751003	41.0	40.0	41.0	38.0	41.0
17	39.87613244224819	41.0	40.0	41.0	38.0	41.0
18	39.85996827157668	41.0	40.0	41.0	38.0	41.0
19	39.87143653216349	41.0	40.0	41.0	38.0	41.0
20	39.83870996937056	41.0	40.0	41.0	38.0	41.0
21	39.8144107033651	41.0	40.0	41.0	38.0	41.0
22	39.75314924641762	41.0	40.0	41.0	38.0	41.0
23	39.68920367346358	41.0	40.0	41.0	37.0	41.0
24	39.659201307407564	41.0	40.0	41.0	37.0	41.0
25	39.654032186119395	41.0	40.0	41.0	37.0	41.0
26	39.54499191274296	41.0	40.0	41.0	37.0	41.0
27	39.49193278332228	41.0	40.0	41.0	36.0	41.0
28	39.40694870571564	41.0	39.0	41.0	36.0	41.0
29	39.342132993035	41.0	39.0	41.0	36.0	41.0
30	39.2560809579036	41.0	39.0	41.0	35.0	41.0
31	39.1834585532925	41.0	39.0	41.0	35.0	41.0
32	39.09637476258632	41.0	39.0	41.0	35.0	41.0
33	38.99936517294835	41.0	39.0	41.0	35.0	41.0
34	38.935216610489	41.0	39.0	41.0	35.0	41.0
35	38.81912212857122	40.0	38.0	41.0	35.0	41.0
36	38.74138490559695	40.0	38.0	41.0	35.0	41.0
37	38.66271160363002	40.0	38.0	41.0	35.0	41.0
38	38.56187236485359	40.0	38.0	41.0	35.0	41.0
39	38.45948937149536	40.0	38.0	41.0	35.0	41.0
40	38.33651781200586	40.0	38.0	41.0	35.0	41.0
41	38.21918082744728	40.0	37.0	41.0	35.0	41.0
42	38.05487827740779	40.0	37.0	41.0	34.0	41.0
43	37.21755044677085	40.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	2.0
15	2.0
16	2.0
17	11.0
18	21.0
19	26.0
20	54.0
21	100.0
22	201.0
23	288.0
24	481.0
25	711.0
26	1100.0
27	1611.0
28	2311.0
29	3314.0
30	4413.0
31	5740.0
32	7235.0
33	9467.0
34	14350.0
35	21332.0
36	34253.0
37	70933.0
38	148609.0
39	446869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22895664687196	17.394131922491628	11.778821600669218	26.598089829967197
2	19.46695214490089	20.046571222811366	32.84447771576039	27.641998916527356
3	19.915727032125353	21.86688284402519	28.95677616463613	29.260613959213334
4	14.05398486499905	15.288471359732311	33.806673829481056	36.85086994578758
5	15.899120680493226	35.346420338255506	32.256713199101675	16.497745782149593
6	34.69051858000437	34.077412698351125	15.921876191916882	15.31019252972762
7	30.96585509652345	29.611772874137454	20.134619536899486	19.287752492439612
8	29.893501620683725	30.455536894312285	19.11553464461968	20.53542684038431
9	28.220971531045112	13.332661011404905	18.55130139545588	39.8950660620941
10	18.75196363255538	24.893365863371255	30.077097224215482	26.27757327985788
11	38.13823714604513	21.09603989454889	19.788373743760012	20.977349215645965
12	23.7947659737872	24.974820250853654	26.72427948422565	24.506134291133495
13	31.437773373207197	17.625048646370303	23.997109015707768	26.940068964714737
14	23.76994177950685	19.641497260934603	23.660172295423425	32.92838866413511
15	27.342815658377713	25.877283147087233	20.74113149194701	26.03876970258805
16	26.64631599906392	24.538974631483544	22.845240542563797	25.969468826888743
17	25.159579488492305	25.33826197023941	23.830580045743748	25.671578495524532
18	25.771651028717198	22.95682012414683	25.530390890555037	25.741137956580936
19	26.01058389866557	24.149286498353458	24.997446469598767	24.842683133382206
20	25.991060704205506	23.04835934055562	25.016969664058834	25.943610291180043
21	27.595712137608785	23.590742127045573	24.3322356384925	24.481310096853147
22	27.045830375763313	23.153991458925656	24.091363378365973	25.708814786945062
23	25.591934205541744	23.186831799275705	24.8676366203411	26.353597374841453
24	25.26818533846884	24.30262761510604	24.471095975248208	25.958091071176913
25	25.551982767871806	23.19083987231055	24.648485530209882	26.608691829607768
26	25.5799099864372	24.30624781010526	24.64189160360416	25.471950599853383
27	26.10276957846708	23.396415231194702	24.185876326381265	26.31493886395695
28	24.825875085171553	23.878159751447754	25.576806962152155	25.719158201228538
29	24.731879307870432	24.068090696228143	25.72251981087067	25.47751018503075
30	24.36779112509196	24.5477665336245	25.752515712292762	25.331926628990782
31	25.458892039320492	24.48105151149606	24.293965005643624	25.76609144353983
32	24.482732316317126	24.044042258019054	24.901769887476583	26.571455538187237
33	24.086579549259866	23.517303885632867	25.943739583858584	26.452376981248683
34	25.60137257107542	23.53928364098526	25.1765168293815	25.68282695855782
35	25.552241353228887	23.918886945188955	25.567885767332655	24.960985934249504
36	24.139589547462695	24.543629167911106	25.22668238865638	26.090098895969817
37	24.64357240842523	23.46442318010858	25.769582345860503	26.12242206560569
38	24.103646182827603	22.79908305632377	26.99721632863096	26.100054432217668
39	24.39558905097881	22.509726041743434	26.754146092969194	26.340538814308562
40	23.948494968575414	22.744780131335503	27.548132431904776	25.758592468184304
41	22.767535642759157	22.744521545978415	28.55338300758043	25.934559803681996
42	23.514718032062	22.71879230294826	28.429132743500134	25.337356921489608
43	22.32406175535498	21.99074523006986	28.784429024137648	26.900763990437515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	79.0
2	89.0
3	273.5
4	458.0
5	458.0
6	553.0
7	648.0
8	644.5
9	641.0
10	962.5
11	1284.0
12	1284.0
13	2230.0
14	3176.0
15	5298.0
16	7420.0
17	6679.5
18	5939.0
19	5939.0
20	6546.0
21	7153.0
22	5555.5
23	3958.0
24	4361.0
25	4764.0
26	4764.0
27	5465.0
28	6166.0
29	7604.5
30	9043.0
31	10914.0
32	12785.0
33	12785.0
34	15921.0
35	19057.0
36	23229.5
37	27402.0
38	31120.5
39	34839.0
40	34839.0
41	37721.5
42	40604.0
43	42604.0
44	44604.0
45	47124.0
46	49644.0
47	49644.0
48	53119.0
49	56594.0
50	57450.5
51	58307.0
52	57280.5
53	56254.0
54	56254.0
55	54934.5
56	53615.0
57	51302.5
58	48990.0
59	48486.5
60	47983.0
61	47983.0
62	44555.5
63	41128.0
64	38185.0
65	35242.0
66	31594.0
67	27946.0
68	27946.0
69	25006.5
70	22067.0
71	19515.5
72	16964.0
73	13302.5
74	9641.0
75	9641.0
76	7951.5
77	6262.0
78	5211.0
79	4160.0
80	3721.5
81	3283.0
82	3283.0
83	2592.0
84	1901.0
85	1753.0
86	1605.0
87	1360.0
88	1115.0
89	1115.0
90	825.5
91	536.0
92	300.0
93	64.0
94	43.0
95	22.0
96	22.0
97	18.5
98	15.0
99	8.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	773439.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.6087945079774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86116551538026	50.30323458399529
2	5.9745457843211325	6.764236691662753
3	1.6828995039351313	2.858007365875229
4	0.798681229712134	1.8084952644061156
5	0.47135059681747266	1.334129453822641
6	0.30552415260885213	1.0377212383355061
7	0.2244381010460694	0.8893619239314328
8	0.18599847744985723	0.8423319671004509
9	0.13841179401769926	0.7051792324525591
>10	1.1039000353062942	12.709072036151383
>50	0.13661104947110986	5.487203677415862
>100	0.1107530448096503	12.106279671859134
>500	0.0048054007044538395	1.7470670668382347
>1k	9.153144198959693E-4	1.407679826153414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.509154568104272	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3106	0.40158305955608653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2812	0.36357101206429987	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.13071489800747055	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	849	0.10976948408342481	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	843	0.10899372801216387	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	839	0.10847655729798988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.2929267854349213E-4	0.0
12	0.0	0.0	0.0	3.8787803563047635E-4	0.0
13	0.0	0.0	0.0	5.171707141739685E-4	0.0
14	0.0	0.0	0.0	6.464633927174606E-4	0.0
15	0.0	0.0	0.0	0.001034341428347937	0.0
16	0.0	0.0	0.0	0.0016808048210653975	0.0
17	0.0	0.0	0.0	0.002327268213782858	0.0
18	0.0	0.0	0.0	0.0027151462494133345	1.2929267854349213E-4
19	0.0	0.0	0.0	0.003103024285043811	1.2929267854349213E-4
20	0.0	0.0	0.0	0.003490902320674287	1.2929267854349213E-4
21	0.0	0.0	0.0	0.004395951070478732	1.2929267854349213E-4
22	0.0	0.0	0.0	0.0056888778559136535	1.2929267854349213E-4
23	0.0	0.0	0.0	0.007240389998435559	1.2929267854349213E-4
24	0.0	0.0	0.0	0.01047270696202286	1.2929267854349213E-4
25	0.0	0.0	0.0	0.013187853211436196	1.2929267854349213E-4
26	0.0	0.0	0.0	0.019911072495697787	1.2929267854349213E-4
27	0.0	0.0	0.0	0.04667465695420066	1.2929267854349213E-4
28	0.0	0.0	0.0	0.1298098492576661	1.2929267854349213E-4
29	0.0	0.0	0.0	0.24164801619778678	1.2929267854349213E-4
30	0.0	0.0	0.0	0.3902053038442592	1.2929267854349213E-4
31	0.0	0.0	0.0	0.7544227793012765	1.2929267854349213E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACT	40	0.0019306189	23.125002	4
CTCCGAT	40	0.0019306189	23.125002	14
TTATGCG	65	2.680963E-6	22.76923	4
ATGCGGA	75	3.7374957E-7	22.2	6
TTCGCCA	50	2.700995E-4	22.2	19
CTTATGC	70	5.09836E-6	21.142859	3
TATGCGG	80	6.954051E-7	20.8125	5
GTATTGA	45	0.0038246934	20.555555	1
AGCGTCA	85	1.2442306E-6	19.588236	3
GCAGTCG	460	0.0	18.902174	9
CTATAGA	50	0.0070328526	18.5	1
AAGACGG	230	0.0	18.5	5
AATATAG	60	9.233093E-4	18.5	5
ATATAGC	60	9.233093E-4	18.5	6
CCGTTAT	50	0.0070328526	18.5	9
GGTATCA	1505	0.0	18.43854	1
AGTCGGT	485	0.0	18.309278	11
AGACGGA	265	0.0	18.150944	6
GACGGAC	245	0.0	18.122448	7
CAGTCGG	515	0.0	17.961166	10
>>END_MODULE