FastQCFastQC Report
Fri 10 Feb 2017
ERR1631417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128694
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA21951.70559622049202No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA12360.960417735092545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.8772747758248248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10340.803456260587129No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA10230.7949088535596066No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA9720.7552799664320015No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC9110.707880709279376No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC8870.6892318212193265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8680.6744681181717873No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT8300.6449407120767091No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT8160.6340621940416803No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT8110.63017700902917No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT8020.6231836760066514No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA5960.4631140534912272No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT5360.4164918333411037No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA5360.4164918333411037No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA5320.4133836853310955No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT4410.34267331810340806No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG4340.33723405908589366No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA4240.3294636890608731No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT4230.328686652058371No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC3570.2774022098932351No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC3500.2719629508757207No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC3280.2548681368206754No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA3130.24321258178314453No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3120.24243554478064247No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT3050.23699628576312803No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA2970.23077998974311156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.22534073072559713No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT2880.22378665672059306No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA2580.20047554664553127ABI Solid3 Adapter B (100% over 21bp)
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2510.19503628762801684No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2440.18959702861050243No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC2370.18415776959298802ABI Solid3 Adapter B (100% over 23bp)
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT2370.18415776959298802No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA2350.18260369558798392No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA2340.18182665858548186No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT2330.18104962158297977No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT2270.17638739956796742No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT2120.16473184453043654No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT1990.15463036349790976No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT1940.15074517848539948No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC1940.15074517848539948No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA1820.14142073445537479No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT1800.13986666045037063No Hit
TCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCAT1770.13753554944286447No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT1760.1367585124403624No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT1740.1352044384353583No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC1710.13287332742785213No Hit
TCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCC1690.131319253422848ABI Solid3 Adapter B (100% over 21bp)
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC1630.12665703140783566No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT1620.1258799944053336No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG1550.12044073538781917No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT1530.11888666138281505No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT1520.118109624380313No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCT1480.11500147637030476No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT1440.11189332836029653No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1420.11033925435529239No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT1400.10878518035028828No Hit
GGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCT1330.10334592133277387No Hit
TCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCAT1330.10334592133277387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCTC200.001835867837.05
ATTAAAT200.001835867837.035
CACCTCG200.001835867837.07
CTTGAAA200.001835867837.027
GGGCAAA251.2275412E-437.011
TTAAATG303.5818928E-430.83333236
ATGCCCC2100.029.9523837
GCAGTTG250.005478441729.626
TATGCTG502.6891394E-729.636
CCTAAGG250.005478441729.63
ATCACCT250.005478441729.629
TCCTAAG250.005478441729.62
TAAATGA250.005478441729.637
ATATGCT453.971998E-628.77777735
TATGCCT405.8998434E-527.7536
GAGGGCA405.8998434E-527.759
TATTGAC1300.027.0384630
ATTGACT1300.027.0384631
TATTGCT358.8264706E-426.42857230
AGAGTGG358.8264706E-426.42857225
Produced by FastQC (version 0.11.5)