##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128694
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96493232007708	34.0	33.0	34.0	31.0	34.0
2	33.07346884858657	34.0	33.0	34.0	31.0	34.0
3	33.048160753415075	34.0	33.0	34.0	31.0	34.0
4	36.38670023466518	37.0	37.0	37.0	35.0	37.0
5	36.41193062613642	37.0	37.0	37.0	35.0	37.0
6	36.47595070477256	37.0	37.0	37.0	35.0	37.0
7	36.499836822229476	37.0	37.0	37.0	35.0	37.0
8	36.52254961381261	37.0	37.0	37.0	35.0	37.0
9	38.32593594106952	39.0	39.0	39.0	37.0	39.0
10	38.30218969027305	39.0	39.0	39.0	37.0	39.0
11	38.3404976144964	39.0	39.0	39.0	37.0	39.0
12	38.34189628110091	39.0	39.0	39.0	37.0	39.0
13	38.332128925979454	39.0	39.0	39.0	37.0	39.0
14	39.77129469905357	41.0	40.0	41.0	38.0	41.0
15	39.72393429375107	41.0	40.0	41.0	37.0	41.0
16	39.57498407074145	41.0	39.0	41.0	37.0	41.0
17	39.41404416678322	41.0	39.0	41.0	37.0	41.0
18	39.6595023855036	41.0	39.0	41.0	38.0	41.0
19	39.721603182743564	41.0	40.0	41.0	38.0	41.0
20	39.80025486813682	41.0	40.0	41.0	38.0	41.0
21	39.84209831072155	41.0	40.0	41.0	38.0	41.0
22	39.78001305422164	41.0	40.0	41.0	38.0	41.0
23	39.7370195968732	41.0	40.0	41.0	38.0	41.0
24	39.71136960542061	41.0	40.0	41.0	37.0	41.0
25	39.69559575426982	41.0	40.0	41.0	37.0	41.0
26	39.609795328453544	41.0	40.0	41.0	37.0	41.0
27	39.54278365735777	41.0	40.0	41.0	37.0	41.0
28	39.430051129034766	41.0	39.0	41.0	36.0	41.0
29	39.33659688874384	41.0	39.0	41.0	36.0	41.0
30	39.21725954589957	41.0	39.0	41.0	35.0	41.0
31	39.06859682658088	41.0	39.0	41.0	35.0	41.0
32	38.9529193280184	40.0	38.0	41.0	35.0	41.0
33	38.86927906506908	40.0	38.0	41.0	35.0	41.0
34	38.766803425179106	40.0	38.0	41.0	35.0	41.0
35	38.64791676379629	40.0	38.0	41.0	35.0	41.0
36	38.56017374547376	40.0	38.0	41.0	35.0	41.0
37	38.476634497334764	40.0	38.0	41.0	35.0	41.0
38	38.348485554882124	40.0	38.0	41.0	35.0	41.0
39	38.1797519697888	40.0	37.0	41.0	35.0	41.0
40	38.07933547795546	40.0	37.0	41.0	35.0	41.0
41	37.88876715309183	40.0	37.0	41.0	34.0	41.0
42	37.74269973736149	40.0	36.0	41.0	34.0	41.0
43	36.92070337389467	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	3.0
14	2.0
15	1.0
16	1.0
17	3.0
18	7.0
19	6.0
20	11.0
21	12.0
22	23.0
23	51.0
24	93.0
25	137.0
26	225.0
27	280.0
28	452.0
29	670.0
30	873.0
31	1142.0
32	1291.0
33	1720.0
34	2357.0
35	3518.0
36	5974.0
37	13737.0
38	27030.0
39	69072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14333224548153	11.437984676830311	16.632477038556576	30.786206039131585
2	19.631839868214524	16.998461466735044	37.88210794598039	25.48759071907004
3	17.547826627504	21.426795343994282	29.983526815546956	31.041851212954764
4	11.403018011717718	19.206023590843397	35.300013986666045	34.09094441077284
5	13.860786050631733	40.485958941364785	30.3021119865728	15.35114302143068
6	26.944535098761403	28.42556762553033	26.09756476603416	18.53233250967411
7	34.490341430058905	30.21430680529007	16.28358742443315	19.011764340217884
8	38.26207904020389	26.291046979657175	16.133619282950253	19.313254697188682
9	34.339596251573504	12.945436461684306	14.177817147652572	38.53715013908962
10	21.004864251635663	23.830947829735653	21.182805725208635	33.98138219342005
11	32.407882263353386	29.688252754596174	13.217399412560027	24.68646556949042
12	27.16288249646448	28.15515874865961	24.311156697281923	20.37080205759398
13	35.08788288498298	17.866411798529846	25.308095171492067	21.737610144995102
14	23.635911542107635	20.762428706855022	22.6801560290301	32.92150372200724
15	38.088799788645936	20.731347226754938	16.97048813464497	24.209364849954156
16	27.483021741495328	24.345346325392015	24.66393149641786	23.507700436694794
17	18.80429546054983	30.675089747773786	26.08668624799913	24.43392854367725
18	27.70447728720842	20.152454659890903	26.46665734222264	25.676410710678045
19	24.496091503877416	32.759102988484315	24.921907781248542	17.82289772638973
20	28.64702317124341	19.275956921068584	30.060453478794656	22.016566428893345
21	34.154661444978004	16.790992587066995	26.204795872379442	22.84955009557555
22	31.48709341538844	17.94023031376754	19.436026543584006	31.136649727260014
23	24.98717888945872	22.240353085613936	20.89374796027787	31.878720064649478
24	24.62197149828275	21.221657575333737	29.669603866536125	24.486767059847388
25	22.62343232784745	21.183582762211138	23.64601302314016	32.54697188680125
26	31.457566009293362	18.846255458684944	21.579871633487187	28.11630689853451
27	26.13097735714175	18.889769530825056	21.25895535145384	33.720297760579356
28	22.30484715682161	20.442289461824174	26.3027025346947	30.950160846659518
29	22.414409374174397	21.15094720810605	31.212799353505215	25.221844064214338
30	21.307131645608965	23.540335990799882	33.60918146922157	21.54335089436959
31	22.601675291777397	26.048611434876527	24.54659890904005	26.80311436430603
32	20.847902777130248	24.482104837832377	32.3402800441357	22.329712340901676
33	25.548976642267707	18.871120642765007	32.04889116819743	23.531011546769857
34	27.476028408472814	23.404354515362023	21.78889458716024	27.330722489004927
35	24.103687817613874	26.024523287798967	24.337575955366994	25.534212939220165
36	18.598380654886785	24.932009262281067	29.103920928714626	27.36568915411752
37	21.65291311172238	25.680295895690552	28.058029123346856	24.608761869240215
38	22.64907454893002	18.078542900212906	32.12193264643262	27.150449904424452
39	24.839541858983324	18.149253267440596	31.09002750710989	25.921177366466193
40	21.809874586227796	17.358229598893498	27.042441761076663	33.78945405380204
41	16.051253360685035	21.401930159914215	32.726467434379224	29.820349045021523
42	17.412622189068642	18.96125693505525	31.63084526085132	31.995275615024788
43	14.998368222294744	25.281675913407	35.23008065644086	24.4898752078574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	5.5
2	11.0
3	57.0
4	103.0
5	103.0
6	134.0
7	165.0
8	159.0
9	153.0
10	213.5
11	274.0
12	274.0
13	531.0
14	788.0
15	1354.0
16	1920.0
17	1749.0
18	1578.0
19	1578.0
20	1760.0
21	1942.0
22	1273.0
23	604.0
24	543.5
25	483.0
26	483.0
27	479.5
28	476.0
29	520.0
30	564.0
31	587.0
32	610.0
33	610.0
34	690.5
35	771.0
36	839.0
37	907.0
38	1126.5
39	1346.0
40	1346.0
41	1873.5
42	2401.0
43	3861.0
44	5321.0
45	7716.0
46	10111.0
47	10111.0
48	16706.0
49	23301.0
50	23041.0
51	22781.0
52	17049.5
53	11318.0
54	11318.0
55	8661.0
56	6004.0
57	5615.5
58	5227.0
59	5164.5
60	5102.0
61	5102.0
62	5140.5
63	5179.0
64	4991.0
65	4803.0
66	4555.5
67	4308.0
68	4308.0
69	3926.5
70	3545.0
71	3104.0
72	2663.0
73	2214.0
74	1765.0
75	1765.0
76	1452.0
77	1139.0
78	863.5
79	588.0
80	446.5
81	305.0
82	305.0
83	202.0
84	99.0
85	64.0
86	29.0
87	18.0
88	7.0
89	7.0
90	4.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	128694.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.88285390150279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62996077526535	48.29518081651048
2	5.332833410244578	5.746965670505229
3	1.5415897554222429	2.4919576670241037
4	0.8104522381172128	1.746779181624629
5	0.5364559298569451	1.44528882465383
6	0.3360059990770651	1.0862977294978786
7	0.2696700507614213	1.0171414362751954
8	0.17593447161975082	0.7583881144420097
9	0.1312298107983387	0.6363933050491865
>10	0.9950392247346562	10.248341025999657
>50	0.1312298107983387	4.945840520925607
>100	0.08508306414397784	9.371066250174835
>500	0.017305029995385326	7.068705611761232
>1k	0.007210429164743885	5.141653845556125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2195	1.70559622049202	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1236	0.960417735092545	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1129	0.8772747758248248	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.803456260587129	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	1023	0.7949088535596066	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	972	0.7552799664320015	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	911	0.707880709279376	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	887	0.6892318212193265	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	868	0.6744681181717873	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	830	0.6449407120767091	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	816	0.6340621940416803	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	811	0.63017700902917	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	802	0.6231836760066514	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	596	0.4631140534912272	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	536	0.4164918333411037	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	536	0.4164918333411037	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	532	0.4133836853310955	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	441	0.34267331810340806	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	434	0.33723405908589366	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	424	0.3294636890608731	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	423	0.328686652058371	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	357	0.2774022098932351	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	350	0.2719629508757207	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	328	0.2548681368206754	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	313	0.24321258178314453	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	312	0.24243554478064247	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	305	0.23699628576312803	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	297	0.23077998974311156	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	290	0.22534073072559713	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	288	0.22378665672059306	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	258	0.20047554664553127	ABI Solid3 Adapter B (100% over 21bp)
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	251	0.19503628762801684	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	244	0.18959702861050243	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	237	0.18415776959298802	ABI Solid3 Adapter B (100% over 23bp)
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	237	0.18415776959298802	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	235	0.18260369558798392	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	234	0.18182665858548186	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	233	0.18104962158297977	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	227	0.17638739956796742	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	212	0.16473184453043654	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	199	0.15463036349790976	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	194	0.15074517848539948	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	194	0.15074517848539948	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	182	0.14142073445537479	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT	180	0.13986666045037063	No Hit
TCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCAT	177	0.13753554944286447	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	176	0.1367585124403624	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	174	0.1352044384353583	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGCGGAGGAATC	171	0.13287332742785213	No Hit
TCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCC	169	0.131319253422848	ABI Solid3 Adapter B (100% over 21bp)
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGAGGCCC	163	0.12665703140783566	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	162	0.1258799944053336	No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG	155	0.12044073538781917	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT	153	0.11888666138281505	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT	152	0.118109624380313	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCT	148	0.11500147637030476	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	144	0.11189332836029653	No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	142	0.11033925435529239	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	140	0.10878518035028828	No Hit
GGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCT	133	0.10334592133277387	No Hit
TCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCAT	133	0.10334592133277387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	7.770370025020592E-4	0.0
14	0.0	0.0	0.0	0.0015540740050041183	0.0
15	0.0	0.0	0.0	0.0023311110075061774	0.0
16	0.0	0.0	0.0	0.007770370025020592	0.0
17	0.0	0.0	0.0	0.020202962065053537	0.0
18	0.0	0.0	0.0	0.022534073072559717	0.0
19	0.0	0.0	0.0	0.025642221082567952	0.0
20	0.0	0.0	0.0	0.028750369092576188	0.0
21	0.0	0.0	0.0	0.032635554105086485	0.0
22	0.0	0.0	0.0	0.05206147916763796	0.0
23	0.0	0.0	0.0	0.08158888526271621	0.0
24	0.0	0.0	0.0	0.1390896234478686	0.0
25	0.0	0.0	0.0	0.15463036349790976	0.0
26	0.0	0.0	0.0	0.17250221455545714	0.0
27	0.0	0.0	0.0	0.20436073165804156	0.0
28	0.0	0.0	0.0	0.3030444309758031	0.0
29	0.0	0.0	0.0	0.5563584937914744	0.0
30	0.0	0.0	0.0	0.9122414409374174	0.0
31	0.0	0.0	0.0	1.8850917680699955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCTC	20	0.0018358678	37.0	5
ATTAAAT	20	0.0018358678	37.0	35
CACCTCG	20	0.0018358678	37.0	7
CTTGAAA	20	0.0018358678	37.0	27
GGGCAAA	25	1.2275412E-4	37.0	11
TTAAATG	30	3.5818928E-4	30.833332	36
ATGCCCC	210	0.0	29.95238	37
GCAGTTG	25	0.0054784417	29.6	26
TATGCTG	50	2.6891394E-7	29.6	36
CCTAAGG	25	0.0054784417	29.6	3
ATCACCT	25	0.0054784417	29.6	29
TCCTAAG	25	0.0054784417	29.6	2
TAAATGA	25	0.0054784417	29.6	37
ATATGCT	45	3.971998E-6	28.777777	35
TATGCCT	40	5.8998434E-5	27.75	36
GAGGGCA	40	5.8998434E-5	27.75	9
TATTGAC	130	0.0	27.03846	30
ATTGACT	130	0.0	27.03846	31
TATTGCT	35	8.8264706E-4	26.428572	30
AGAGTGG	35	8.8264706E-4	26.428572	25
>>END_MODULE