FastQCFastQC Report
Fri 10 Feb 2017
ERR1631416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421869
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.1639642771760157No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA34470.14232809454186002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29010.11978352256046879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28890.11928803746197668No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC27520.1136312492541917No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC27440.11330092585519695No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26930.11119511418660546No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24860.10264799623761649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.024.0282341
ATACACA15600.023.12499837
TATACAC25950.016.82466337
AAGACGG8100.016.216055
CTTATAC73650.016.15139237
CGCTTAT1353.9771294E-715.07407526
GACGGAC8050.014.9378877
GTACTAT2500.014.8000011
GTATCAA34250.014.82
AGACGGA8550.014.713456
ACGGACC8100.014.6172848
TATACAG1904.5656634E-1014.6052645
TATAGTG1803.3360266E-914.3888895
CAAGACG9650.014.3782394
CGGTGAT4100.013.98780514
TACCCCG2650.013.9622645
TCGGTGA4300.013.76744213
CGGACCA8900.013.71919
AGTCGGT4050.013.70370411
TAGCGCC2457.2759576E-1213.59183629
Produced by FastQC (version 0.11.5)