##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2421869
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07497886962507	34.0	33.0	34.0	31.0	34.0
2	33.21102049697981	34.0	33.0	34.0	31.0	34.0
3	33.297206413724275	34.0	34.0	34.0	31.0	34.0
4	36.54071462989947	37.0	37.0	37.0	35.0	37.0
5	36.52532940468704	37.0	37.0	37.0	35.0	37.0
6	36.57341334316596	37.0	37.0	37.0	35.0	37.0
7	36.57018567065353	37.0	37.0	37.0	35.0	37.0
8	36.56749848980271	37.0	37.0	37.0	35.0	37.0
9	38.41547416478761	39.0	39.0	39.0	37.0	39.0
10	38.37734039289491	39.0	39.0	39.0	37.0	39.0
11	38.43397103641857	39.0	39.0	39.0	37.0	39.0
12	38.38734258541647	39.0	39.0	39.0	37.0	39.0
13	38.41625703124323	39.0	39.0	39.0	37.0	39.0
14	39.96716461542718	41.0	40.0	41.0	38.0	41.0
15	39.9684210830561	41.0	40.0	41.0	38.0	41.0
16	39.946966165387146	41.0	40.0	41.0	38.0	41.0
17	39.9311919843724	41.0	40.0	41.0	38.0	41.0
18	39.90934315605014	41.0	40.0	41.0	38.0	41.0
19	39.926599250413624	41.0	40.0	41.0	38.0	41.0
20	39.90434784044884	41.0	40.0	41.0	38.0	41.0
21	39.8726033489012	41.0	40.0	41.0	38.0	41.0
22	39.822508153826654	41.0	40.0	41.0	38.0	41.0
23	39.76071992333194	41.0	40.0	41.0	38.0	41.0
24	39.735231344057006	41.0	40.0	41.0	37.0	41.0
25	39.71133162033124	41.0	40.0	41.0	37.0	41.0
26	39.6367384032745	41.0	40.0	41.0	37.0	41.0
27	39.58297207652437	41.0	40.0	41.0	37.0	41.0
28	39.503899261273006	41.0	40.0	41.0	37.0	41.0
29	39.44806221971543	41.0	39.0	41.0	36.0	41.0
30	39.379570488742374	41.0	39.0	41.0	36.0	41.0
31	39.33834530273933	41.0	39.0	41.0	36.0	41.0
32	39.27260599148839	41.0	39.0	41.0	35.0	41.0
33	39.20207822966478	41.0	39.0	41.0	35.0	41.0
34	39.145813419305505	41.0	39.0	41.0	35.0	41.0
35	39.05925258550318	41.0	39.0	41.0	35.0	41.0
36	39.00474922466905	41.0	39.0	41.0	35.0	41.0
37	38.95081278136844	41.0	39.0	41.0	35.0	41.0
38	38.88560694240687	40.0	39.0	41.0	35.0	41.0
39	38.812747510290606	40.0	38.0	41.0	35.0	41.0
40	38.71591774782203	40.0	38.0	41.0	35.0	41.0
41	38.63783136082092	40.0	38.0	41.0	35.0	41.0
42	38.52001367538872	40.0	38.0	41.0	35.0	41.0
43	37.710554534535106	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	2.0
10	2.0
11	0.0
12	1.0
13	4.0
14	1.0
15	9.0
16	8.0
17	6.0
18	34.0
19	55.0
20	122.0
21	241.0
22	432.0
23	748.0
24	1229.0
25	1868.0
26	2835.0
27	4071.0
28	5754.0
29	7901.0
30	10656.0
31	13953.0
32	18450.0
33	25390.0
34	39093.0
35	59612.0
36	99902.0
37	195014.0
38	474037.0
39	1460438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99282909191207	17.97892454133564	12.526647807953278	26.50159855879901
2	19.616255049302833	19.516084478557676	33.45961321607403	27.40804725606546
3	19.880348606799128	21.270514631468508	28.742842820978343	30.10629394075402
4	14.711984834852752	15.477674473722566	33.36699879308088	36.4433418983438
5	16.71494205508225	34.063403099011545	32.26859091057361	16.953063935332587
6	35.05168941837894	33.854101935323506	15.694242752188496	15.399965894109055
7	30.672220504081764	29.24964149588603	20.277232170691313	19.80090582934089
8	28.329979862659787	31.394307454284277	19.49444829592352	20.781264387132417
9	27.307587652346182	14.278352792822401	19.231965064997322	39.18209448983409
10	19.28242196419377	24.232524550254368	30.101586832318343	26.383466653233516
11	38.099418259203944	20.708717110628196	20.193330027346647	20.99853460282121
12	23.964095498146264	24.46973804115747	26.611100765565766	24.955065695130497
13	30.869340992431876	17.80971638020058	24.169432781046375	27.15150984632117
14	24.049566677636154	19.5773594690712	23.125817292347357	33.24725656094529
15	26.89414662807939	25.76617480136209	20.833785807572582	26.505892762985944
16	26.892288558960043	24.347807416503535	22.578058515964322	26.1818455085721
17	25.786985175498756	24.66723014333145	23.37124757780045	26.17453710336934
18	26.150175752693478	22.910198693653538	24.737589027317334	26.202036526335654
19	26.22619142488714	24.05559507966781	23.97470713733897	25.74350635810607
20	26.63926083533007	22.824933966288018	23.829736455605154	26.706068742776758
21	28.37077480243564	23.45444778392225	23.318891319059784	24.855886094582324
22	27.870334852958607	23.015117663259243	23.133456020949108	25.981091462833046
23	26.174660974643967	22.96639496190752	24.007533025114075	26.851411038334444
24	25.810644588951753	24.075786097431365	23.67939801863767	26.43417129497921
25	26.031052876931	22.86003082743121	23.964673564094507	27.144242731543283
26	25.90478675766526	24.25023814252546	24.178805707492852	25.66616939231643
27	26.83464712583546	22.99740407098815	23.571795171415133	26.596153631761254
28	25.493451545067053	23.61432430903571	24.632174572613135	26.260049573284107
29	25.17464817461225	23.73881494003185	24.863029338085585	26.22350754727031
30	24.825372470600186	24.062160257222832	25.011303253809352	26.101164018367633
31	25.985014053196103	23.415428332415996	23.720729733936892	26.878827880451006
32	24.94321534319156	22.88009797392014	24.520277521203663	27.656409161684632
33	24.663637876367382	22.38407609990466	25.61084022298481	27.34144580074315
34	25.901772556649433	21.973318953254697	25.758040587661846	26.366867902434027
35	25.63759641830338	21.771739099018156	26.25183277873411	26.338831703944347
36	24.125995254078564	22.227791841755273	26.380246000093315	27.265966904072847
37	24.463503187001447	20.99766750389885	27.005217870991373	27.533611438108334
38	23.44073936286397	20.387931799779427	28.632597386563845	27.538731450792753
39	23.741663979348182	20.037954158544498	29.036004837586177	27.184377024521144
40	22.925063246608303	21.058653461438254	29.509936334293883	26.50634695765956
41	21.73482545918049	21.11518005309123	30.693113459068183	26.456881028660096
42	22.188813680673896	21.69163567476193	30.083584207073134	26.03596643749104
43	20.674858962231234	20.8828388323233	30.212162590131836	28.230139615313625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	118.5
2	137.0
3	355.0
4	573.0
5	573.0
6	736.0
7	899.0
8	915.5
9	932.0
10	1410.0
11	1888.0
12	1888.0
13	2960.0
14	4032.0
15	6248.5
16	8465.0
17	8078.5
18	7692.0
19	7692.0
20	8804.0
21	9916.0
22	9519.0
23	9122.0
24	10982.0
25	12842.0
26	12842.0
27	15716.5
28	18591.0
29	23004.0
30	27417.0
31	33751.0
32	40085.0
33	40085.0
34	50739.5
35	61394.0
36	74109.0
37	86824.0
38	100030.5
39	113237.0
40	113237.0
41	121229.0
42	129221.0
43	137829.5
44	146438.0
45	153310.5
46	160183.0
47	160183.0
48	168443.5
49	176704.0
50	180567.0
51	184430.0
52	188714.5
53	192999.0
54	192999.0
55	185780.0
56	178561.0
57	173049.0
58	167537.0
59	163564.5
60	159592.0
61	159592.0
62	147882.0
63	136172.0
64	126029.0
65	115886.0
66	103467.0
67	91048.0
68	91048.0
69	77565.5
70	64083.0
71	55230.0
72	46377.0
73	35492.5
74	24608.0
75	24608.0
76	19670.0
77	14732.0
78	12252.0
79	9772.0
80	8616.0
81	7460.0
82	7460.0
83	5908.5
84	4357.0
85	4000.5
86	3644.0
87	3093.0
88	2542.0
89	2542.0
90	1836.0
91	1130.0
92	644.5
93	159.0
94	109.0
95	59.0
96	59.0
97	39.5
98	20.0
99	14.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2421869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.83956554161094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.78945786313638	47.26546270484822
2	6.806428501430818	7.329103068141464
3	1.9798975057220594	3.1979046457498446
4	0.9020094972714433	1.942551977900056
5	0.49959254881794163	1.3448922888092014
6	0.33009164110933914	1.0663194327746506
7	0.2309635475382658	0.8704483938786602
8	0.1812852630960221	0.7808255843349171
9	0.1443549145653103	0.6994805295593401
>10	0.8997027556068518	9.472214907935962
>50	0.1128874469356477	4.22018904027018
>100	0.10318811576631673	11.551333921586071
>500	0.013707724036787385	4.930206997092848
>1k	0.006432674966983983	5.329066507118715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3971	0.1639642771760157	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3447	0.14232809454186002	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2901	0.11978352256046879	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2889	0.11928803746197668	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2752	0.1136312492541917	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2744	0.11330092585519695	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2693	0.11119511418660546	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2486	0.10264799623761649	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.258084974868583E-5	0.0
2	0.0	4.1290424874342915E-5	0.0	8.258084974868583E-5	0.0
3	0.0	4.1290424874342915E-5	0.0	8.258084974868583E-5	0.0
4	0.0	4.1290424874342915E-5	0.0	8.258084974868583E-5	0.0
5	0.0	8.258084974868583E-5	0.0	8.258084974868583E-5	0.0
6	0.0	8.258084974868583E-5	0.0	8.258084974868583E-5	0.0
7	0.0	8.258084974868583E-5	0.0	8.258084974868583E-5	0.0
8	0.0	8.258084974868583E-5	0.0	8.258084974868583E-5	0.0
9	0.0	8.258084974868583E-5	0.0	1.6516169949737166E-4	0.0
10	0.0	8.258084974868583E-5	0.0	3.303233989947433E-4	0.0
11	0.0	8.258084974868583E-5	0.0	4.95485098492115E-4	0.0
12	0.0	8.258084974868583E-5	0.0	6.193563731151438E-4	4.1290424874342915E-5
13	0.0	8.258084974868583E-5	0.0	6.606467979894866E-4	4.1290424874342915E-5
14	0.0	8.258084974868583E-5	0.0	8.258084974868583E-4	4.1290424874342915E-5
15	0.0	8.258084974868583E-5	0.0	9.49679772109887E-4	4.1290424874342915E-5
16	0.0	8.258084974868583E-5	0.0	0.0013212935959789733	1.2387127462302875E-4
17	0.0	8.258084974868583E-5	0.0	0.001940649969094117	1.2387127462302875E-4
18	0.0	8.258084974868583E-5	0.0	0.002394844642711889	1.2387127462302875E-4
19	0.0	8.258084974868583E-5	0.0	0.0026425871919579466	1.2387127462302875E-4
20	0.0	8.258084974868583E-5	0.0	0.0030967818655757186	1.2387127462302875E-4
21	0.0	8.258084974868583E-5	0.0	0.003798719088439548	2.0645212437171457E-4
22	0.0	8.258084974868583E-5	0.0	0.005202593534167207	2.0645212437171457E-4
23	0.0	1.2387127462302875E-4	0.0	0.008712279648486355	2.0645212437171457E-4
24	0.0	1.2387127462302875E-4	0.0	0.015236166778632536	2.0645212437171457E-4
25	0.0	1.2387127462302875E-4	0.0	0.02043876031279974	2.0645212437171457E-4
26	0.0	1.2387127462302875E-4	0.0	0.03431234307057896	2.0645212437171457E-4
27	0.0	1.2387127462302875E-4	0.0	0.11173188970997193	2.0645212437171457E-4
28	0.0	1.2387127462302875E-4	0.0	0.45266692789742136	2.0645212437171457E-4
29	0.0	1.2387127462302875E-4	0.0	0.8966215761463564	2.0645212437171457E-4
30	0.0	1.2387127462302875E-4	0.0	1.5144914939660237	2.0645212437171457E-4
31	0.0	1.2387127462302875E-4	0.0	2.8483786695316717	2.0645212437171457E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2125	0.0	24.028234	1
ATACACA	1560	0.0	23.124998	37
TATACAC	2595	0.0	16.824663	37
AAGACGG	810	0.0	16.21605	5
CTTATAC	7365	0.0	16.151392	37
CGCTTAT	135	3.9771294E-7	15.074075	26
GACGGAC	805	0.0	14.937887	7
GTACTAT	250	0.0	14.800001	1
GTATCAA	3425	0.0	14.8	2
AGACGGA	855	0.0	14.71345	6
ACGGACC	810	0.0	14.617284	8
TATACAG	190	4.5656634E-10	14.605264	5
TATAGTG	180	3.3360266E-9	14.388889	5
CAAGACG	965	0.0	14.378239	4
CGGTGAT	410	0.0	13.987805	14
TACCCCG	265	0.0	13.962264	5
TCGGTGA	430	0.0	13.767442	13
CGGACCA	890	0.0	13.7191	9
AGTCGGT	405	0.0	13.703704	11
TAGCGCC	245	7.2759576E-12	13.591836	29
>>END_MODULE