FastQCFastQC Report
Fri 10 Feb 2017
ERR1631415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214069
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.6329734805132925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9890.46200056990970195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9820.4587305962096333No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.169571493303561No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3510.1639658241034433No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3180.14855023380311955No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2620.12239044420257018No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT2510.11725191410246229No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2450.11444907950240345No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG2380.11117910580233477No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2360.11024482760231515No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2310.10790913210226609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG250.0054864829.636
CTAACCT250.0054864829.64
GCCCTAC358.8457356E-426.4285721
GACGGTA451.318304E-424.6666668
ACGGTAT451.318304E-424.6666669
AGTTTGC400.001926085323.12524
GTTCAGA400.001926085323.1251
CAAAGTC400.001926085323.12532
CGACGGT603.708082E-521.5833347
TGGTCGG603.708082E-521.58333437
GGTATCA5350.020.4018691
TGACGAA555.1243266E-420.18181821
AGTGACG555.1243266E-420.18181819
GACGAAA555.1243266E-420.18181822
CCGGGAA656.8686524E-519.92307731
ATACCGT656.8686524E-519.9230776
TACCGTC656.8686524E-519.9230777
GCAGTCG2100.019.3809539
AGTCGGT2050.018.95121811
GGGCAGT2150.018.9302337
Produced by FastQC (version 0.11.5)