FastQCFastQC Report
Fri 10 Feb 2017
ERR1631414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317045
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55841.7612641738554462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45501.4351275055591477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39441.2439874465769842No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.5030831585421628No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7150.2255200365878661No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5770.181993092463215No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5330.16811493636550015No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.16590704789540917No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.16559163525682474No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.1640145720639026No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5080.16022962040088948No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA4740.14950559068901892No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4550.14351275055591478No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4550.14351275055591478No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4540.14319733791733036No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.1211184532164204No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG3770.11891056474632938No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3680.11607185099906953No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.11544102572190068No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.1088173603116277No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC3400.10724029711870556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGT250.005490415329.59999826
GCTGCAA250.005490415329.5999981
TAAGTAA250.005490415329.59999826
TTCTGCG250.005490415329.5999982
GTAACAT250.005490415329.59999829
TAGATGC405.9267742E-527.7537
ATTGACT556.237733E-726.9090931
ACAGCAC358.8551745E-426.428573
CATGATT551.8970288E-523.54545432
CCATGAT502.696125E-422.19999931
TATTGAC603.7153393E-521.58333230
ATACACA1900.021.42105337
CAAACAC450.003819789320.55555714
ACACGAA450.003819789320.55555717
CATATCC450.003819789320.55555734
CCGCTAT450.003819789320.55555725
CACCTTA450.003819789320.5555572
ATATCCT450.003819789320.55555735
AAACACG450.003819789320.55555715
ATGGTGC450.003819789320.55555716
Produced by FastQC (version 0.11.5)