##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631414.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317045
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98863883675819	34.0	33.0	34.0	31.0	34.0
2	33.09832042769954	34.0	33.0	34.0	31.0	34.0
3	33.14805153842514	34.0	33.0	34.0	31.0	34.0
4	36.40056458862307	37.0	37.0	37.0	35.0	37.0
5	36.420962323960325	37.0	37.0	37.0	35.0	37.0
6	36.51080445993471	37.0	37.0	37.0	35.0	37.0
7	36.51638095538488	37.0	37.0	37.0	35.0	37.0
8	36.53495560567112	37.0	37.0	37.0	35.0	37.0
9	38.34585626646059	39.0	39.0	39.0	37.0	39.0
10	38.33160907757574	39.0	39.0	39.0	37.0	39.0
11	38.38011954139002	39.0	39.0	39.0	37.0	39.0
12	38.35558674636093	39.0	39.0	39.0	37.0	39.0
13	38.3784541626583	39.0	39.0	39.0	37.0	39.0
14	39.81983945496696	41.0	40.0	41.0	38.0	41.0
15	39.79073317667839	41.0	40.0	41.0	38.0	41.0
16	39.721578955668754	41.0	40.0	41.0	37.0	41.0
17	39.769796085729155	41.0	40.0	41.0	38.0	41.0
18	39.803570471068774	41.0	40.0	41.0	38.0	41.0
19	39.82999889605576	41.0	40.0	41.0	38.0	41.0
20	39.80997650175843	41.0	40.0	41.0	38.0	41.0
21	39.78205932911732	41.0	40.0	41.0	37.0	41.0
22	39.68580170007412	41.0	40.0	41.0	37.0	41.0
23	39.59765648409532	41.0	40.0	41.0	37.0	41.0
24	39.55335362487975	41.0	40.0	41.0	36.0	41.0
25	39.53389266507909	41.0	40.0	41.0	36.0	41.0
26	39.37696856913057	41.0	39.0	41.0	35.0	41.0
27	39.26222775946632	41.0	39.0	41.0	35.0	41.0
28	39.10107713416077	41.0	39.0	41.0	35.0	41.0
29	38.96072481824347	41.0	39.0	41.0	35.0	41.0
30	38.75932438612815	40.0	38.0	41.0	35.0	41.0
31	38.56274345913041	40.0	37.0	41.0	35.0	41.0
32	38.371338453531834	40.0	37.0	41.0	35.0	41.0
33	38.17824914444322	40.0	36.0	41.0	35.0	41.0
34	38.037937832168936	40.0	36.0	41.0	35.0	41.0
35	37.819829992587806	40.0	35.0	41.0	35.0	41.0
36	37.65103376492296	40.0	35.0	41.0	34.0	41.0
37	37.45596997271681	40.0	35.0	41.0	34.0	41.0
38	37.250137993029384	40.0	35.0	41.0	33.0	41.0
39	36.99978551940576	40.0	35.0	41.0	33.0	41.0
40	36.7373653582299	40.0	35.0	41.0	33.0	41.0
41	36.46265356652841	39.0	35.0	41.0	32.0	41.0
42	36.138832026999324	39.0	35.0	41.0	31.0	41.0
43	35.231273163115645	38.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	3.0
13	3.0
14	3.0
15	0.0
16	4.0
17	7.0
18	8.0
19	12.0
20	26.0
21	60.0
22	107.0
23	166.0
24	301.0
25	490.0
26	791.0
27	1144.0
28	1763.0
29	2399.0
30	2981.0
31	3667.0
32	4471.0
33	5657.0
34	8145.0
35	11531.0
36	18362.0
37	43867.0
38	62300.0
39	148771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92218770206122	17.025027992871674	13.634026715450487	23.418757589616614
2	20.420760459871627	21.40894825655664	31.61475500323298	26.555536280338753
3	21.154094844580424	23.672349351038495	28.779195382358967	26.39436042202211
4	14.078443123215948	16.976454446529672	31.655758646248955	37.289343784005425
5	14.82439401346812	37.596240281348074	30.14619375798388	17.433171947199924
6	31.082023056663882	32.861581163557226	17.955810689334324	18.100585090444575
7	29.41096689744358	31.50215269125834	19.018120456086677	20.068759955211405
8	32.607989402135345	28.1669794508666	18.290148086233813	20.934883060764246
9	29.578766421170492	12.913624248923655	19.11463672349351	38.39297260641234
10	21.48433187717832	26.006718289201846	25.690044000063082	26.818905833556748
11	35.74508350549607	23.627560756359507	17.739437619265406	22.887918118879025
12	24.278572442397767	28.683309940229307	24.230945133971517	22.807172483401413
13	32.55436925357599	18.401488747654117	25.025469570565694	24.018672428204198
14	24.162816004037282	21.553407245028307	24.527748426879466	29.756028324054945
15	29.129934236464855	25.920926051506882	21.427242189594537	23.521897522433726
16	24.745698560141307	25.238688514248764	25.019476730432586	24.99613619517734
17	22.951316059234493	27.690390953965526	24.633727073443833	24.724565913356148
18	23.49319497232254	22.52929394880853	28.360958223596022	25.61655285527291
19	23.35567506189973	26.426847923796302	28.390607011622954	21.826870002681005
20	24.546988597833117	22.87782491444432	29.051081076818747	23.524105410903815
21	27.404942516046617	23.388162563673927	27.535207935781987	21.67168698449747
22	26.512009336214103	23.12195429670867	26.281442697408885	24.084593669668344
23	23.660048258133703	24.16123894084436	27.490103928464414	24.688608872557523
24	22.401551830181834	25.61718368055008	28.195366588339194	23.78589790092889
25	22.753236922203474	24.611963601381508	27.430175527133372	25.204623949281647
26	23.97230677033229	25.343405510258798	27.50839786150231	23.175889857906608
27	24.00100932044347	24.30254380293018	26.916368338879337	24.78007853774701
28	21.982053020864544	25.324796164582313	29.117317730921478	23.57583308363166
29	22.00539355611979	25.42131243198915	29.527669573719816	23.045624438171238
30	21.041807945244365	26.533141982999258	30.257534419404188	22.16751565235219
31	22.687315680739328	26.543550600072546	27.625731363055717	23.143402356132412
32	21.590310523742687	26.048983582772163	29.037202920721032	23.323502972764118
33	21.167973000678135	25.334889369017016	29.5462789193963	23.950858710908545
34	22.666498446592755	25.49953476635809	27.856928827138105	23.977037959911055
35	22.235644782286425	25.864151776561688	28.651768676370864	23.248434764781027
36	20.251699285590373	26.493084577899033	28.83691589521992	24.41830024129067
37	21.244618271854154	25.223864120235294	29.515053068176446	24.016464539734105
38	20.59329117317731	23.546815120881895	30.980144774401108	24.879748931539687
39	21.171442539702564	23.52158210979514	31.14920594868236	24.15776940181993
40	20.362409121733506	23.968521818669274	30.769449131826715	24.899619927770505
41	18.543109022378527	24.514185683420333	32.65403964736867	24.28866564683247
42	19.342364648551467	24.336292955258717	31.845952467315364	24.47538992887445
43	17.644813827690076	24.15335362487975	32.603889037833746	25.597943509596426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	60.5
2	77.0
3	308.0
4	539.0
5	539.0
6	748.5
7	958.0
8	981.5
9	1005.0
10	1645.0
11	2285.0
12	2285.0
13	3898.5
14	5512.0
15	8302.5
16	11093.0
17	9817.0
18	8541.0
19	8541.0
20	9154.5
21	9768.0
22	6797.0
23	3826.0
24	3454.5
25	3083.0
26	3083.0
27	2857.5
28	2632.0
29	2916.0
30	3200.0
31	3546.0
32	3892.0
33	3892.0
34	4722.0
35	5552.0
36	6559.5
37	7567.0
38	8921.0
39	10275.0
40	10275.0
41	11361.0
42	12447.0
43	14169.0
44	15891.0
45	17847.0
46	19803.0
47	19803.0
48	23268.5
49	26734.0
50	25669.0
51	24604.0
52	22867.5
53	21131.0
54	21131.0
55	19173.5
56	17216.0
57	16621.0
58	16026.0
59	15974.0
60	15922.0
61	15922.0
62	15358.0
63	14794.0
64	14208.5
65	13623.0
66	12630.0
67	11637.0
68	11637.0
69	10275.0
70	8913.0
71	7864.5
72	6816.0
73	5273.5
74	3731.0
75	3731.0
76	3070.0
77	2409.0
78	2061.5
79	1714.0
80	1516.5
81	1319.0
82	1319.0
83	1119.5
84	920.0
85	809.5
86	699.0
87	621.5
88	544.0
89	544.0
90	383.0
91	222.0
92	139.0
93	56.0
94	34.5
95	13.0
96	13.0
97	10.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	317045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.876322748198746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.96402013686019	38.51960487034856
2	7.314457434829615	6.711208200164105
3	2.582334918734711	3.554040905274515
4	1.3589401911257069	2.493727152143273
5	0.827780096005274	1.8987753424436447
6	0.62383695784824	1.7171607372300188
7	0.43830128938033114	1.407535598879461
8	0.35129817769851557	1.2893014864760939
9	0.2596402193422733	1.0720204650863323
>10	1.917857479187841	17.437895921652064
>50	0.22518347308354272	7.231412782118651
>100	0.12877464668691893	10.482547292419353
>500	0.00482044131983119	1.2333523591695594
>1k	0.0020659034227847955	3.1873186351592433
>5k	6.886344742615986E-4	1.7640982514351484
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5584	1.7612641738554462	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4550	1.4351275055591477	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3944	1.2439874465769842	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1595	0.5030831585421628	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	715	0.2255200365878661	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	577	0.181993092463215	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	533	0.16811493636550015	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	526	0.16590704789540917	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	525	0.16559163525682474	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	520	0.1640145720639026	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	508	0.16022962040088948	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	474	0.14950559068901892	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	455	0.14351275055591478	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	455	0.14351275055591478	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	454	0.14319733791733036	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.1211184532164204	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	377	0.11891056474632938	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	368	0.11607185099906953	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	366	0.11544102572190068	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.1088173603116277	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	340	0.10724029711870556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.1541263858442806E-4	0.0
11	0.0	0.0	0.0	0.0018924758315065685	0.0
12	0.0	0.0	0.0	0.0028387137472598526	0.0
13	0.0	0.0	0.0	0.0031541263858442807	0.0
14	0.0	0.0	0.0	0.004100364301597565	0.0
15	0.0	0.0	0.0	0.004731189578766421	0.0
16	0.0	0.0	0.0	0.007569903326026274	0.0
17	0.0	0.0	0.0	0.008831553880363986	0.0
18	0.0	0.0	0.0	0.010408617073286127	0.0
19	0.0	0.0	0.0	0.01135485498903941	0.0
20	0.0	0.0	0.0	0.01324733082054598	0.0
21	0.0	0.0	0.0	0.01640145720639026	0.0
22	0.0	0.0	0.0	0.02270970997807882	0.0
23	0.0	0.0	0.0	0.03154126385844281	0.0
24	0.0	0.0	0.0	0.04857354634200192	0.0
25	0.0	0.0	0.0	0.05803592549953477	0.0
26	0.0	0.0	0.0	0.08263811130912016	0.0
27	0.0	0.0	0.0	0.17095365011276	0.0
28	0.0	0.0	0.0	0.39899698780930154	0.0
29	0.0	0.0	0.0	0.7371193363718084	0.0
30	0.0	0.0	0.0	1.2092920563326972	0.0
31	0.0	0.0	0.0	2.140390165433929	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTGT	25	0.0054904153	29.599998	26
GCTGCAA	25	0.0054904153	29.599998	1
TAAGTAA	25	0.0054904153	29.599998	26
TTCTGCG	25	0.0054904153	29.599998	2
GTAACAT	25	0.0054904153	29.599998	29
TAGATGC	40	5.9267742E-5	27.75	37
ATTGACT	55	6.237733E-7	26.90909	31
ACAGCAC	35	8.8551745E-4	26.42857	3
CATGATT	55	1.8970288E-5	23.545454	32
CCATGAT	50	2.696125E-4	22.199999	31
TATTGAC	60	3.7153393E-5	21.583332	30
ATACACA	190	0.0	21.421053	37
CAAACAC	45	0.0038197893	20.555557	14
ACACGAA	45	0.0038197893	20.555557	17
CATATCC	45	0.0038197893	20.555557	34
CCGCTAT	45	0.0038197893	20.555557	25
CACCTTA	45	0.0038197893	20.555557	2
ATATCCT	45	0.0038197893	20.555557	35
AAACACG	45	0.0038197893	20.555557	15
ATGGTGC	45	0.0038197893	20.555557	16
>>END_MODULE