FastQCFastQC Report
Fri 10 Feb 2017
ERR1631413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262141
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT185300.8191355003954218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143440.6340895638247129No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT131050.5793184421307072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.22748360955395794No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA33600.1485318554413717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG659.400355E-825.61538710
CAACCGT400.001931761623.1250026
CTCGTAT753.7440805E-722.2000039
GGTATCA93700.018.440771
CGTATGC953.6095116E-617.52631611
CAGTCGG27900.017.50537710
GCAGTCG27450.017.4553749
AGTCGGT27850.017.33752311
TATTACG650.001580572917.0769257
TCGGTGA29250.016.6341913
GGCAGTC28800.016.5729168
TACGCTA2800.016.51785926
GGGCAGT28950.016.4870457
CGGTGAT29750.016.29243714
ATTACGG803.3843756E-416.18758
CTTATAC69700.015.73959937
TATACAC22450.015.65701537
GTCGGTG30900.015.62621312
TTCCTCG21850.015.4942820
GTGATTC31150.015.44141316
Produced by FastQC (version 0.11.5)