FastQCFastQC Report
Fri 10 Feb 2017
ERR1631412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613868
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.16215700416638784No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA24720.1531723784101302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20670.12807738922885886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20640.1278915004201087No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT19420.12033202219760228No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18530.11481732087134759No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT17060.10570876924258985No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC16720.10360202941008807No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16610.10292043711133747No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT16280.10087566021508573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG401.6005979E-632.37513
GGTATCA12250.022.653061
ATACACA5500.022.237
ATCCGTC603.7274876E-521.5833347
ACTGATC4250.021.3294128
TCGTATG705.1040315E-621.14285910
CTCGTAT656.9043395E-519.9230779
GTGCTAG656.9043395E-519.9230771
GTCTAGA5450.019.6880741
CTAGACA4900.019.6326544
GTTCTAG5300.019.547171
ACATGTA4300.019.3604648
TCTAGAC4900.019.2551043
ACGGAGT4050.018.72839526
CGTATGC701.21958474E-418.511
GACATGT4450.017.8764067
AAGACGG6700.017.6716425
AGCAATT4650.017.50537716
TAGAACT5950.017.4117664
CAAGACG6750.017.2666684
Produced by FastQC (version 0.11.5)