##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1613868
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06742682796858	34.0	33.0	34.0	31.0	34.0
2	33.20663771758285	34.0	33.0	34.0	31.0	34.0
3	33.293194362859914	34.0	34.0	34.0	31.0	34.0
4	36.5113038984601	37.0	37.0	37.0	35.0	37.0
5	36.51053741693868	37.0	37.0	37.0	35.0	37.0
6	36.56625820699091	37.0	37.0	37.0	35.0	37.0
7	36.57000324685786	37.0	37.0	37.0	35.0	37.0
8	36.566519070952516	37.0	37.0	37.0	35.0	37.0
9	38.40937734684621	39.0	39.0	39.0	37.0	39.0
10	38.37300076586189	39.0	39.0	39.0	37.0	39.0
11	38.42602492892851	39.0	39.0	39.0	37.0	39.0
12	38.38880193423502	39.0	39.0	39.0	37.0	39.0
13	38.40836673135597	39.0	39.0	39.0	37.0	39.0
14	39.971989654668164	41.0	40.0	41.0	38.0	41.0
15	39.96228873736886	41.0	40.0	41.0	38.0	41.0
16	39.95275202185061	41.0	40.0	41.0	38.0	41.0
17	39.94037678422275	41.0	40.0	41.0	38.0	41.0
18	39.925607298738186	41.0	40.0	41.0	38.0	41.0
19	39.934872616595655	41.0	40.0	41.0	38.0	41.0
20	39.91065378333296	41.0	40.0	41.0	38.0	41.0
21	39.87882714075749	41.0	40.0	41.0	38.0	41.0
22	39.83584531076891	41.0	40.0	41.0	38.0	41.0
23	39.78613368627422	41.0	40.0	41.0	38.0	41.0
24	39.7621614654978	41.0	40.0	41.0	38.0	41.0
25	39.747681346925525	41.0	40.0	41.0	38.0	41.0
26	39.6699792052386	41.0	40.0	41.0	37.0	41.0
27	39.622382375758114	41.0	40.0	41.0	37.0	41.0
28	39.552817206859544	41.0	40.0	41.0	37.0	41.0
29	39.50885884099567	41.0	40.0	41.0	37.0	41.0
30	39.4540080105684	41.0	39.0	41.0	37.0	41.0
31	39.41000936879596	41.0	39.0	41.0	36.0	41.0
32	39.33977871796206	41.0	39.0	41.0	36.0	41.0
33	39.27194045609678	41.0	39.0	41.0	35.0	41.0
34	39.222953178326854	41.0	39.0	41.0	35.0	41.0
35	39.142758887343945	41.0	39.0	41.0	35.0	41.0
36	39.079644679738365	41.0	39.0	41.0	35.0	41.0
37	39.03595151524164	41.0	39.0	41.0	35.0	41.0
38	38.96697995127235	41.0	39.0	41.0	35.0	41.0
39	38.9027597052547	40.0	39.0	41.0	35.0	41.0
40	38.813292660861975	40.0	38.0	41.0	35.0	41.0
41	38.731758111567984	40.0	38.0	41.0	35.0	41.0
42	38.61002324849368	40.0	38.0	41.0	35.0	41.0
43	37.82107768417244	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	3.0
15	4.0
16	3.0
17	11.0
18	13.0
19	44.0
20	80.0
21	143.0
22	297.0
23	490.0
24	773.0
25	1160.0
26	1765.0
27	2590.0
28	3786.0
29	5196.0
30	7085.0
31	9251.0
32	12128.0
33	16352.0
34	24886.0
35	37352.0
36	62511.0
37	124513.0
38	313153.0
39	990278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20175627746507	17.546354472608662	12.009904155730208	26.241985094196057
2	19.67217888947547	20.019976850647016	33.36233198749836	26.94551227237915
3	20.204254623054673	20.8266103547502	28.6452795395906	30.32385548260452
4	14.65925342097371	15.137669251760366	33.115595575350646	37.08748175191528
5	16.60049025075161	33.96721417117137	32.08620531542852	17.346090262648495
6	35.46931967174515	33.5864519279148	15.662681210607063	15.281547189732988
7	31.09492226130018	29.323216025102422	19.81277279182684	19.769088921770553
8	28.197163584630218	31.704141850510698	19.675215073351723	20.42347949150736
9	27.2950451957657	14.561351981698628	19.24618370275636	38.897419119779315
10	18.69173934919089	24.514582357417087	30.528395135165947	26.265283158226076
11	37.72576195822707	20.910817985114026	20.410962978384852	20.95245707827406
12	24.42114224955201	24.338421729658187	26.700944563000196	24.53949145778961
13	30.530068134444697	18.198576339576718	24.725194377731015	26.546161148247567
14	24.46042675113454	19.426433884307762	23.053062580087094	33.060076784470596
15	26.243038464112306	26.230274099244795	21.161458062245487	26.36522937439741
16	26.898296514956616	24.418911583847006	22.49310352519537	26.189688376001012
17	25.577556528786744	24.886298011981154	23.407614501309894	26.12853095792221
18	26.37749803577492	22.806450093811886	24.812933895461093	26.0031179749521
19	25.969162285887077	23.90412350948157	24.089268762996728	26.03744544163463
20	26.134355473929716	23.585386165411297	23.943841751617853	26.336416609041137
21	27.842673626343668	23.549261773577516	24.102652757226736	24.50541184285208
22	27.426654472360813	22.980875759355783	23.667858833560118	25.924610934723287
23	25.762825708174397	23.177484156077202	24.395551556880736	26.664138578867664
24	25.57594549244424	23.936220310459095	23.849843977326522	26.637990219770142
25	26.119980072719702	23.513880936978737	23.939566309016598	26.42657268128496
26	26.030691481583375	24.19745604968932	24.174901540894297	25.596950927833007
27	26.712283780333955	23.158337608775934	23.953941710226612	26.175436900663502
28	25.352507144326548	23.391008434394884	24.8217326324086	26.43475178886997
29	24.863743503186132	23.63737306892509	24.830345480547354	26.66853794734142
30	24.64798855916345	24.396976704414488	25.216932239811435	25.738102496610626
31	26.02300807748837	23.610543117528817	23.845072831235267	26.521375973747542
32	25.44910736194038	23.302339472621057	24.160402213811786	27.088150951626776
33	24.93215058480619	22.78792317587312	25.208877058098928	27.071049181221763
34	26.221475362297287	22.463361315795343	24.8853685679374	26.429794753969965
35	25.822557978719452	22.55581001668042	25.736863237885625	25.8847687667145
36	24.455159901553287	22.750745414123088	26.049961954757144	26.744132729566484
37	25.511751890489187	22.089353032590026	26.031558962690877	26.36733611422991
38	24.325657364790676	21.50566217311453	27.388299414822033	26.780381047272762
39	24.749731700486038	21.42281772734821	27.210651676593127	26.616798895572625
40	23.84637405289652	22.570309343762933	27.4710818976521	26.112234705688447
41	23.54027714782126	22.20993290653263	28.339430486260337	25.910359459385774
42	24.14100781476552	22.522969660467894	27.791987944491126	25.54403458027546
43	22.60129081188796	21.85909876148483	27.981780418225032	27.557830008402174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	133.0
2	148.0
3	356.5
4	565.0
5	565.0
6	687.0
7	809.0
8	780.5
9	752.0
10	1030.0
11	1308.0
12	1308.0
13	2028.0
14	2748.0
15	4012.5
16	5277.0
17	5094.5
18	4912.0
19	4912.0
20	5959.0
21	7006.0
22	6773.5
23	6541.0
24	7584.0
25	8627.0
26	8627.0
27	9896.5
28	11166.0
29	13502.0
30	15838.0
31	19131.5
32	22425.0
33	22425.0
34	29047.5
35	35670.0
36	45422.0
37	55174.0
38	62589.0
39	70004.0
40	70004.0
41	76214.5
42	82425.0
43	89477.5
44	96530.0
45	104075.5
46	111621.0
47	111621.0
48	117461.5
49	123302.0
50	126905.0
51	130508.0
52	133704.5
53	136901.0
54	136901.0
55	131806.5
56	126712.0
57	121228.5
58	115745.0
59	112990.0
60	110235.0
61	110235.0
62	104627.5
63	99020.0
64	88119.5
65	77219.0
66	67622.0
67	58025.0
68	58025.0
69	46967.5
70	35910.0
71	30698.5
72	25487.0
73	19019.5
74	12552.0
75	12552.0
76	9832.5
77	7113.0
78	5709.5
79	4306.0
80	4024.5
81	3743.0
82	3743.0
83	3109.5
84	2476.0
85	2444.5
86	2413.0
87	2041.5
88	1670.0
89	1670.0
90	1205.5
91	741.0
92	412.5
93	84.0
94	57.0
95	30.0
96	30.0
97	19.5
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1613868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.95744127538714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.52597219746745	35.46493514720247
2	7.350824734530794	6.168435942494781
3	2.4657344390379192	3.103677237798995
4	1.2800715811861103	2.1483411278363276
5	0.7938275796591244	1.665348702816521
6	0.537877006771965	1.3540765755009443
7	0.39855282204520404	1.170557963827103
8	0.29844595339381674	1.0017622850718395
9	0.23701234191147555	0.8949988275563443
>10	1.6416133920050642	13.824144568543318
>50	0.24256057401002806	7.143360297700547
>100	0.20328551389476235	17.310251163229587
>500	0.01812924796648986	5.17826507687328
>1k	0.0060926161198859375	3.571845083548053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2617	0.16215700416638784	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2472	0.1531723784101302	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2067	0.12807738922885886	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2064	0.1278915004201087	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1942	0.12033202219760228	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1853	0.11481732087134759	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1706	0.10570876924258985	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1672	0.10360202941008807	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1661	0.10292043711133747	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	1628	0.10087566021508573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.196293625005267E-5
2	0.0	0.0	0.0	0.0	6.196293625005267E-5
3	0.0	0.0	0.0	0.0	6.196293625005267E-5
4	0.0	0.0	0.0	0.0	6.196293625005267E-5
5	0.0	0.0	0.0	0.0	6.196293625005267E-5
6	0.0	0.0	0.0	0.0	6.196293625005267E-5
7	0.0	0.0	0.0	6.196293625005267E-5	6.196293625005267E-5
8	0.0	0.0	0.0	6.196293625005267E-5	6.196293625005267E-5
9	0.0	0.0	0.0	1.2392587250010533E-4	6.196293625005267E-5
10	0.0	0.0	0.0	3.71777617500316E-4	6.196293625005267E-5
11	0.0	0.0	0.0	4.957034900004213E-4	6.196293625005267E-5
12	0.0	0.0	0.0	7.43555235000632E-4	6.196293625005267E-5
13	0.0	0.0	0.0	8.674811075007374E-4	6.196293625005267E-5
14	0.0	0.0	0.0	0.001115332852500948	6.196293625005267E-5
15	0.0	0.0	0.0	0.0013012216612511061	6.196293625005267E-5
16	0.0	0.0	0.0	0.0016110363425013694	1.2392587250010533E-4
17	0.0	0.0	0.0	0.0018588880875015801	1.2392587250010533E-4
18	0.0	0.0	0.0	0.002106739832501791	1.2392587250010533E-4
19	0.0	0.0	0.0	0.002292628641251949	1.2392587250010533E-4
20	0.0	0.0	0.0	0.0026644062587522646	1.2392587250010533E-4
21	0.0	0.0	0.0	0.0034699244300029496	1.2392587250010533E-4
22	0.0	0.0	0.0	0.004523294346253845	1.2392587250010533E-4
23	0.0	6.196293625005267E-5	0.0	0.0078073299675066364	1.2392587250010533E-4
24	0.0	1.2392587250010533E-4	0.0	0.012578476058760692	1.2392587250010533E-4
25	0.0	1.2392587250010533E-4	0.0	0.015490734062513167	1.2392587250010533E-4
26	0.0	1.2392587250010533E-4	0.0	0.02274039760376933	1.2392587250010533E-4
27	0.0	1.2392587250010533E-4	0.0	0.06958437740880914	1.2392587250010533E-4
28	0.0	1.2392587250010533E-4	0.0	0.27561114044023427	1.85888808750158E-4
29	0.0	1.2392587250010533E-4	0.0	0.5774326029142408	1.85888808750158E-4
30	0.0	1.2392587250010533E-4	0.0	0.991778757618343	1.85888808750158E-4
31	0.0	1.2392587250010533E-4	0.0	1.784718452810267	1.85888808750158E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	40	1.6005979E-6	32.375	13
GGTATCA	1225	0.0	22.65306	1
ATACACA	550	0.0	22.2	37
ATCCGTC	60	3.7274876E-5	21.583334	7
ACTGATC	425	0.0	21.329412	8
TCGTATG	70	5.1040315E-6	21.142859	10
CTCGTAT	65	6.9043395E-5	19.923077	9
GTGCTAG	65	6.9043395E-5	19.923077	1
GTCTAGA	545	0.0	19.688074	1
CTAGACA	490	0.0	19.632654	4
GTTCTAG	530	0.0	19.54717	1
ACATGTA	430	0.0	19.360464	8
TCTAGAC	490	0.0	19.255104	3
ACGGAGT	405	0.0	18.728395	26
CGTATGC	70	1.21958474E-4	18.5	11
GACATGT	445	0.0	17.876406	7
AAGACGG	670	0.0	17.671642	5
AGCAATT	465	0.0	17.505377	16
TAGAACT	595	0.0	17.411766	4
CAAGACG	675	0.0	17.266668	4
>>END_MODULE