FastQCFastQC Report
Fri 10 Feb 2017
ERR1631411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221951
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71073.202058111925605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55302.4915409256998164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48972.2063428414379747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.9168690386616866No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA13450.6059896103193949No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9490.42757185144468823No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA8490.38251686182986333No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6970.3140332776153295No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.3036706300039198No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.30051678073088206No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6290.2833958846772486No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT6020.2712310374812459No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT6000.27032993768894936No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA5830.26267058945442917No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT5810.26176948966213265No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT5730.25816509049294667No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC5620.2532090416353159No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC5310.2392419948547202No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.23698924537397895No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA5210.2347364958932377No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.22797824745101397No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA4670.21040680150123228No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA4300.19373645534374703No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA4160.18742875679767157No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4080.18382435762848556No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3740.1685056611594451No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3680.1658023617825556No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT3650.16445071209411086No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.16264851250951787No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.16219796261336963No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG3220.14507706655973615No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.14057156759825368No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC3080.13876936801366066No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT3020.13606606863677118No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2980.1342638690521782No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.13291221936373343No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.12029682227158248No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT2670.12029682227158248No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2570.11579132331009999No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.11443967362165523No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA2420.10903307486787625No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC2390.1076814251794315No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2360.10632977549098674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2340.10542867569869026No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT2320.10452757590639376No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA2240.10092317673720776No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT2220.10002207694491128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCC200.001838738237.04
CTCGTAT401.5923779E-632.3759
TCGTATG401.5923779E-632.37510
CTATGAC250.005486910729.63
AAGTTCC250.005486910729.631
ATGCCCC1000.029.637
GTATGCC453.9891293E-628.77777712
GGACCGT556.223563E-726.909096
GAAATCT509.048008E-625.9000024
TATGCCG509.048008E-625.90000213
CCGTCTT509.048008E-625.90000217
CGTATGC551.893707E-523.54545411
TCTTCTG551.893707E-523.54545420
TCTCGTA551.893707E-523.5454548
CTTGAAA551.893707E-523.54545427
ACCGTTA551.893707E-523.5454548
ATGTGTA952.7830538E-1023.36842211
GTGTTGA400.001926307823.1251
ATCTCGT652.6666858E-622.7692287
TATTGAC1300.022.76922830
Produced by FastQC (version 0.11.5)