##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631411.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221951
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95253456844078	34.0	33.0	34.0	31.0	34.0
2	33.030655414933925	34.0	33.0	34.0	31.0	34.0
3	33.03644498109943	34.0	33.0	34.0	31.0	34.0
4	36.35852958535893	37.0	37.0	37.0	35.0	37.0
5	36.38658082189312	37.0	37.0	37.0	35.0	37.0
6	36.478713770156475	37.0	37.0	37.0	35.0	37.0
7	36.48094849764137	37.0	37.0	37.0	35.0	37.0
8	36.52060589950034	37.0	37.0	37.0	35.0	37.0
9	38.317908006722206	39.0	39.0	39.0	37.0	39.0
10	38.303084915138925	39.0	39.0	39.0	37.0	39.0
11	38.35107298457768	39.0	39.0	39.0	37.0	39.0
12	38.345251879919445	39.0	39.0	39.0	37.0	39.0
13	38.36378299714802	39.0	39.0	39.0	37.0	39.0
14	39.740226446377804	41.0	40.0	41.0	37.0	41.0
15	39.69058035332123	41.0	39.0	41.0	37.0	41.0
16	39.59445102747904	41.0	39.0	41.0	37.0	41.0
17	39.64953075228316	41.0	39.0	41.0	37.0	41.0
18	39.77271559938905	41.0	40.0	41.0	38.0	41.0
19	39.8250199368329	41.0	40.0	41.0	38.0	41.0
20	39.81475190469968	41.0	40.0	41.0	38.0	41.0
21	39.78359637938104	41.0	40.0	41.0	37.0	41.0
22	39.68482683114742	41.0	40.0	41.0	37.0	41.0
23	39.60058301156562	41.0	40.0	41.0	36.0	41.0
24	39.54223229451546	41.0	40.0	41.0	36.0	41.0
25	39.525751179314355	41.0	40.0	41.0	36.0	41.0
26	39.307067776220876	41.0	39.0	41.0	35.0	41.0
27	39.165527526345905	41.0	39.0	41.0	35.0	41.0
28	38.998751976787666	41.0	38.0	41.0	35.0	41.0
29	38.78177615780059	40.0	38.0	41.0	35.0	41.0
30	38.50558456596276	40.0	37.0	41.0	35.0	41.0
31	38.1910872219544	40.0	36.0	41.0	35.0	41.0
32	37.94189257989376	40.0	35.0	41.0	35.0	41.0
33	37.69873981194047	40.0	35.0	41.0	35.0	41.0
34	37.48583696401458	40.0	35.0	41.0	35.0	41.0
35	37.26103509333141	40.0	35.0	41.0	34.0	41.0
36	37.01835540276908	40.0	35.0	41.0	33.0	41.0
37	36.74337579015188	39.0	35.0	41.0	33.0	41.0
38	36.45475352667931	39.0	35.0	41.0	33.0	41.0
39	36.124225617365994	39.0	35.0	41.0	32.0	41.0
40	35.76634932935648	39.0	35.0	41.0	30.0	41.0
41	35.33487121031219	38.0	35.0	41.0	25.0	41.0
42	34.868200638879756	38.0	35.0	41.0	21.0	41.0
43	33.907745403264684	37.0	35.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	3.0
14	2.0
15	0.0
16	4.0
17	8.0
18	8.0
19	7.0
20	24.0
21	38.0
22	73.0
23	168.0
24	239.0
25	377.0
26	615.0
27	1022.0
28	1487.0
29	2127.0
30	2981.0
31	3394.0
32	3806.0
33	4693.0
34	6456.0
35	8965.0
36	13994.0
37	39635.0
38	41120.0
39	90698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.788255065307204	14.627552928349049	16.120675284184347	22.463516722159397
2	18.896062644457558	22.191384584885853	33.76601141693437	25.146541353722217
3	20.457218034611245	24.91991475595965	29.547963289194463	25.074903920234647
4	13.42863965469856	18.803699915747167	30.399953142810798	37.36770728674347
5	13.160111916594202	40.93426026465301	28.508094128884302	17.397533689868485
6	27.16275213898563	31.63220710877626	21.493933345648365	19.711107406589743
7	29.405589522011617	33.08117557478903	17.806632995571096	19.70660190762826
8	35.88765087789647	28.130082766015924	17.031236624299957	18.951029731787646
9	32.40399908087821	12.405440840545886	17.58676464625075	37.60379543232516
10	21.59755982176246	28.519808426184156	22.46712112132858	27.4155106307248
11	32.75137305080851	27.177620285558525	15.539015368256958	24.531991295376006
12	24.76492559168465	31.660591752233604	24.20399097098008	19.37049168510167
13	35.13388090164045	19.084392501047528	25.50067357209474	20.281053025217275
14	23.62278160494884	22.30582425850751	27.2735874134381	26.79780672310555
15	31.23301990078891	25.374069051277083	22.103076805240796	21.289834242693207
16	21.745790737595236	26.829345215835925	27.57770859333817	23.847155453230666
17	18.072006884402413	31.630404909191668	27.59978553824943	22.697802668156484
18	20.986614162585436	23.346594518609965	31.98994372631797	23.67684759248663
19	20.709525976454263	29.876864713382684	33.09514262156962	16.31846668859343
20	23.341187919856186	23.73091358002442	34.48599015097927	18.441908349140128
21	26.268861145027504	22.353131997603075	32.241350568368695	19.136656289000726
22	24.25265035976409	23.616023356506616	29.186171722587417	22.945154561141873
23	20.80999860329532	26.171091817563337	29.84442512086001	23.174484458281334
24	19.783645939869608	26.243179800947058	33.62543984933611	20.347734409847217
25	19.16729368193881	26.07287194020302	31.03387684669139	23.725957531166788
26	22.733846659848346	24.980288442043513	30.763546909002436	21.522317989105705
27	20.911822879824825	25.031651130204413	29.740798644745915	24.315727345224847
28	18.965897878360536	26.184157764551635	32.73515325454717	22.11479110254065
29	18.990227572752545	26.817180368639924	34.31793503971598	19.874657018891558
30	18.213029001896818	27.835873683831114	35.865123383089056	18.08597393118301
31	18.56220517141171	29.569139134313428	31.81242706723556	20.056228627039303
32	17.806182445674946	28.929808831679065	35.34338660334939	17.9206221192966
33	19.267766308779866	26.971268433122624	34.806781677036824	18.954183581060686
34	19.766525043815978	28.12602781695059	30.478348824740596	21.62909831449284
35	18.67754594482566	29.388018076061833	30.87032723438957	21.064108744722933
36	16.118422534703605	28.878446143518165	32.52609810273439	22.477033219043843
37	17.002851980842618	28.631995350325074	32.99466999472857	21.370482674103744
38	17.804380246090354	25.373618501380935	34.66936395871161	22.152637293817104
39	18.446864397997757	25.34793715730049	35.27084807006952	20.93435037463224
40	17.485390919617394	25.12040945974562	32.93564795833315	24.458551662303844
41	14.306761402291496	25.959783916269807	36.34315682290235	23.390297858536343
42	14.604574883645489	25.157805101125923	34.77839703357948	25.459222981649106
43	13.439903402102265	27.601587737834027	36.663047249167604	22.295461610896098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14.0
1	44.5
2	75.0
3	322.0
4	569.0
5	569.0
6	802.0
7	1035.0
8	1128.0
9	1221.0
10	2011.5
11	2802.0
12	2802.0
13	4745.5
14	6689.0
15	10259.5
16	13830.0
17	12054.0
18	10278.0
19	10278.0
20	11068.0
21	11858.0
22	8049.0
23	4240.0
24	3729.0
25	3218.0
26	3218.0
27	2953.0
28	2688.0
29	2699.0
30	2710.0
31	2787.5
32	2865.0
33	2865.0
34	2791.5
35	2718.0
36	2678.5
37	2639.0
38	3001.5
39	3364.0
40	3364.0
41	4107.5
42	4851.0
43	6460.5
44	8070.0
45	10644.0
46	13218.0
47	13218.0
48	18504.5
49	23791.0
50	22559.0
51	21327.0
52	17270.5
53	13214.0
54	13214.0
55	11147.0
56	9080.0
57	8772.0
58	8464.0
59	8404.0
60	8344.0
61	8344.0
62	8184.5
63	8025.0
64	7788.5
65	7552.0
66	7154.0
67	6756.0
68	6756.0
69	6235.0
70	5714.0
71	4975.0
72	4236.0
73	3552.0
74	2868.0
75	2868.0
76	2350.5
77	1833.0
78	1423.5
79	1014.0
80	744.0
81	474.0
82	474.0
83	330.5
84	187.0
85	132.5
86	78.0
87	55.0
88	32.0
89	32.0
90	20.0
91	8.0
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	221951.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.78108784265155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09886491522978	44.73756198098176
2	6.3401642540448	6.439208758429765
3	1.828487463947569	2.78557747577726
4	0.9010982224106965	1.8303499194837896
5	0.5475301569701843	1.390208849880186
6	0.3260068033767573	0.9932988071746286
7	0.27813100661146123	0.9886656554951199
8	0.19738000120438384	0.8018536943633987
9	0.17644512554845082	0.8064067877895206
>10	1.0806007558378583	10.50048953194018
>50	0.11264564740903599	4.152003749524899
>100	0.09171150054540983	8.899826617946506
>500	0.015949826181810407	5.0874954502225345
>1k	0.0029905924090894514	4.189970133481783
>5k	0.001993728272726301	6.397082587508674
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7107	3.202058111925605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5530	2.4915409256998164	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4897	2.2063428414379747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2035	0.9168690386616866	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1345	0.6059896103193949	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	949	0.42757185144468823	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	849	0.38251686182986333	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	697	0.3140332776153295	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	674	0.3036706300039198	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.30051678073088206	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	629	0.2833958846772486	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	602	0.2712310374812459	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	600	0.27032993768894936	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	583	0.26267058945442917	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	581	0.26176948966213265	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	573	0.25816509049294667	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	562	0.2532090416353159	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	531	0.2392419948547202	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	526	0.23698924537397895	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	521	0.2347364958932377	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	506	0.22797824745101397	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	467	0.21040680150123228	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	430	0.19373645534374703	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	416	0.18742875679767157	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	408	0.18382435762848556	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	374	0.1685056611594451	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	368	0.1658023617825556	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	365	0.16445071209411086	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.16264851250951787	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.16219796261336963	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	322	0.14507706655973615	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	312	0.14057156759825368	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	308	0.13876936801366066	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	302	0.13606606863677118	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	298	0.1342638690521782	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	295	0.13291221936373343	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	267	0.12029682227158248	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	267	0.12029682227158248	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	257	0.11579132331009999	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.11443967362165523	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	242	0.10903307486787625	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	239	0.1076814251794315	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	236	0.10632977549098674	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	234	0.10542867569869026	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	232	0.10452757590639376	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	224	0.10092317673720776	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	222	0.10002207694491128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.010997922964979E-4	0.0
11	0.0	0.0	0.0	0.0018021995845929957	0.0
12	0.0	0.0	0.0	0.0031538492730377424	0.0
13	0.0	0.0	0.0	0.0031538492730377424	0.0
14	0.0	0.0	0.0	0.004956048857630738	0.0
15	0.0	0.0	0.0	0.00810989813066848	0.0
16	0.0	0.0	0.0	0.018021995845929957	0.0
17	0.0	0.0	0.0	0.028835193353487932	0.0
18	0.0	0.0	0.0	0.03379124221111867	0.0
19	0.0	0.0	0.0	0.03829674117260116	0.0
20	0.0	0.0	0.0	0.04280224013408365	0.0
21	0.0	0.0	0.0	0.05406598753778987	0.0
22	0.0	0.0	0.0	0.07298908317601632	0.0
23	0.0	0.0	0.0	0.09641767777572527	0.0
24	0.0	0.0	0.0	0.1288572702983992	0.0
25	0.0	0.0	0.0	0.1464287162481809	0.0
26	0.0	0.0	0.0	0.16400016219796262	0.0
27	0.0	0.0	0.0	0.23608814558168245	0.0
28	0.0	0.0	0.0	0.4717257412672166	0.0
29	0.0	0.0	0.0	0.8839788962428644	0.0
30	0.0	0.0	0.0	1.3854409306558655	0.0
31	0.0	0.0	0.0	2.363584755193714	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTTCC	20	0.0018387382	37.0	4
CTCGTAT	40	1.5923779E-6	32.375	9
TCGTATG	40	1.5923779E-6	32.375	10
CTATGAC	25	0.0054869107	29.6	3
AAGTTCC	25	0.0054869107	29.6	31
ATGCCCC	100	0.0	29.6	37
GTATGCC	45	3.9891293E-6	28.777777	12
GGACCGT	55	6.223563E-7	26.90909	6
GAAATCT	50	9.048008E-6	25.900002	4
TATGCCG	50	9.048008E-6	25.900002	13
CCGTCTT	50	9.048008E-6	25.900002	17
CGTATGC	55	1.893707E-5	23.545454	11
TCTTCTG	55	1.893707E-5	23.545454	20
TCTCGTA	55	1.893707E-5	23.545454	8
CTTGAAA	55	1.893707E-5	23.545454	27
ACCGTTA	55	1.893707E-5	23.545454	8
ATGTGTA	95	2.7830538E-10	23.368422	11
GTGTTGA	40	0.0019263078	23.125	1
ATCTCGT	65	2.6666858E-6	22.769228	7
TATTGAC	130	0.0	22.769228	30
>>END_MODULE