FastQCFastQC Report
Fri 10 Feb 2017
ERR1631410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629477
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.17109441647589985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10070.15997407371516353No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8490.13487387148378732No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT7860.12486556299912467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7590.12057628793426924No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7000.11120342760736295No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6700.10643756642419024No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6540.1038957737931648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC250.005494478629.5999983
TATGCCG556.254195E-726.9090913
TCGTATG601.3358003E-624.66666810
TACTATA551.9008869E-523.5454542
TCTATGC400.001930221723.1250023
CGTATGC652.6796442E-622.7692311
GGTATCA6250.022.21
AACGTCG450.00382391420.55555526
ACTTACC450.00382391420.5555558
TTAGTCA450.00382391420.5555554
TAAGATG450.00382391420.5555555
ATACACA2700.019.8703737
ATGCCGT759.254851E-619.73333414
CTCGTAT902.1490705E-618.59
TGCCGTC902.1490705E-618.515
ATGTATG500.007031433718.4999987
TGTGTAT1002.8698014E-718.4999988
GTGTATA1054.788744E-717.6190479
TCTCGTA852.7199474E-517.4117648
TATTAGA650.001578860617.0769232
Produced by FastQC (version 0.11.5)