FastQCFastQC Report
Fri 10 Feb 2017
ERR1631409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477156
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28130.5895346595243484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22960.4811843506107017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19940.4178926808004091No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8850.18547393305334106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.17520475483908826No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8190.17164197872393933No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6900.1446067952619269No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6810.14272061967155394No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT6610.13852911835961407No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6550.13727166796603207No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6390.13391846691648016No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6320.13245144145730117No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC5950.12469716403021233No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5160.1081407338480497No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC5010.10499710786409475No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG4980.10436838266730376No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG4980.10436838266730376No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC4850.10164390681454284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG250.005493161329.64
CGCCTAT405.93296E-527.75000236
TCTAATA509.076544E-625.9000022
CTATTGC509.076544E-625.90000210
GTACTAT751.3695171E-824.6666661
ATAGTAC451.3216282E-424.6666663
TCTATCG451.3216282E-424.66666631
AAGACGG1650.023.5454565
GTTCATA400.001929540923.1250021
AGAGCGA1700.022.8529415
CGCAAGA1800.022.611112
TATCGCC502.6988934E-422.233
CAAGACG2100.022.023814
CGGACCA1750.021.1428579
CAGAGCG1950.020.87179614
CGTATGC450.003822578620.55555511
TTATGGG450.003822578620.5555552
ATGCTAG450.003822578620.55555515
TATACAG450.003822578620.5555555
AGACGGA1900.020.4473696
Produced by FastQC (version 0.11.5)