##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631409.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477156
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05890316793669	34.0	33.0	34.0	31.0	34.0
2	33.1828249042242	34.0	33.0	34.0	31.0	34.0
3	33.25490615228563	34.0	34.0	34.0	31.0	34.0
4	36.4932412041345	37.0	37.0	37.0	35.0	37.0
5	36.48900150055747	37.0	37.0	37.0	35.0	37.0
6	36.546728952376164	37.0	37.0	37.0	35.0	37.0
7	36.552058446294296	37.0	37.0	37.0	35.0	37.0
8	36.55498411421003	37.0	37.0	37.0	35.0	37.0
9	38.39711540879712	39.0	39.0	39.0	37.0	39.0
10	38.359251481695715	39.0	39.0	39.0	37.0	39.0
11	38.41015936087988	39.0	39.0	39.0	37.0	39.0
12	38.374395375935755	39.0	39.0	39.0	37.0	39.0
13	38.3999677254399	39.0	39.0	39.0	37.0	39.0
14	39.92884297797785	41.0	40.0	41.0	38.0	41.0
15	39.917188508580004	41.0	40.0	41.0	38.0	41.0
16	39.875210622940926	41.0	40.0	41.0	38.0	41.0
17	39.87264751988867	41.0	40.0	41.0	38.0	41.0
18	39.859771227858396	41.0	40.0	41.0	38.0	41.0
19	39.888346788052544	41.0	40.0	41.0	38.0	41.0
20	39.857403029617146	41.0	40.0	41.0	38.0	41.0
21	39.82673171876703	41.0	40.0	41.0	38.0	41.0
22	39.766384578628376	41.0	40.0	41.0	38.0	41.0
23	39.70174534114629	41.0	40.0	41.0	37.0	41.0
24	39.66989412267686	41.0	40.0	41.0	37.0	41.0
25	39.65988900904526	41.0	40.0	41.0	37.0	41.0
26	39.55961153165841	41.0	40.0	41.0	37.0	41.0
27	39.50317506224379	41.0	40.0	41.0	36.0	41.0
28	39.402792797324146	41.0	39.0	41.0	36.0	41.0
29	39.34691170183336	41.0	39.0	41.0	36.0	41.0
30	39.26070090284938	41.0	39.0	41.0	35.0	41.0
31	39.18667270242855	41.0	39.0	41.0	35.0	41.0
32	39.09415998122208	41.0	39.0	41.0	35.0	41.0
33	38.994033397882454	41.0	39.0	41.0	35.0	41.0
34	38.93282909572551	41.0	39.0	41.0	35.0	41.0
35	38.80642599066133	41.0	38.0	41.0	35.0	41.0
36	38.72683985950088	40.0	38.0	41.0	35.0	41.0
37	38.64328228084735	40.0	38.0	41.0	35.0	41.0
38	38.53778848007779	40.0	38.0	41.0	35.0	41.0
39	38.42344642003873	40.0	38.0	41.0	35.0	41.0
40	38.29062403071532	40.0	38.0	41.0	35.0	41.0
41	38.15979889176705	40.0	37.0	41.0	35.0	41.0
42	37.9971204385987	40.0	37.0	41.0	34.0	41.0
43	37.1762086194033	40.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	0.0
16	2.0
17	11.0
18	6.0
19	26.0
20	42.0
21	64.0
22	126.0
23	195.0
24	331.0
25	487.0
26	715.0
27	1039.0
28	1399.0
29	1985.0
30	2783.0
31	3590.0
32	4419.0
33	5943.0
34	8782.0
35	12883.0
36	21065.0
37	43898.0
38	91383.0
39	275980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30354014200806	17.18201175296968	12.12957607155731	26.384872033464944
2	19.544341892379013	20.159025559775003	32.73101459480757	27.56561795303842
3	20.059686978682024	22.03828517298326	29.01566783190403	28.886360016430686
4	14.035870868227581	15.357241656816639	33.751016439068145	36.85587103588763
5	15.613551962041763	35.865419275876235	32.22530996152202	16.295718800559985
6	34.10016849835274	33.90002431070761	16.555172731769066	15.444634459170587
7	30.830378324908413	29.83678293891306	19.956366471342708	19.37647226483582
8	30.260334146484585	30.123900778780943	19.145730117613528	20.47003495712094
9	28.282364677380144	13.388283915532865	18.710442706368564	39.61890870071842
10	18.944118904509217	25.03290328529873	29.748551836296727	26.274425973895333
11	37.538876174668246	21.465306943641075	19.36578393649037	21.630032945200313
12	23.718448473874375	25.307027471099598	26.739892194586258	24.234631860439773
13	31.28075514087636	17.642029021955082	24.883685838593667	26.19352999857489
14	23.89637770456622	19.949240919112405	23.500071255522304	32.65431012079907
15	27.424154783760446	26.027756121687666	20.802630586223373	25.74545850832851
16	26.624835483573506	24.733210941495024	23.056400841653463	25.585552733278007
17	25.024101132543652	25.628725196790985	23.886527676483162	25.460645994182197
18	25.677765762140687	22.644376262689768	25.87686207445783	25.800995900711715
19	25.667915734057623	24.393489760162296	25.245831551945276	24.692762953834805
20	26.062755157642364	22.528481251414632	25.490405653496968	25.918357937446036
21	27.96045737662316	23.135620216449126	24.501211343879152	24.402711063048564
22	27.272003286137032	22.72380521255103	24.156669936037687	25.84752156527425
23	25.30723704616519	22.87595671017445	25.19595268633319	26.620853557327163
24	24.81704096773382	24.2117881782897	25.392324522797576	25.5788463311789
25	25.31038067214915	23.235797097804493	24.903385894759786	26.55043633528657
26	25.763901114101053	24.626956383237346	24.788748333878228	24.820394168783373
27	26.44250517650412	22.867992857681767	24.386364207931997	26.303137757882116
28	24.558425336787128	23.78760824552138	25.809798053466793	25.844168364224696
29	24.19858494915709	23.91964053684749	26.26667169646824	25.61510281752718
30	23.670036633721466	25.09829070576499	26.494479792772175	24.737192867741367
31	25.354181860858922	24.69800233047473	24.467469758318032	25.480346050348313
32	24.39202273470312	23.986285407707335	25.312685997870716	26.30900585971883
33	23.93703526729204	23.42064230566104	26.113472323516838	26.528850103530083
34	25.60252831359136	23.732490003269373	25.352924410465338	25.312057272673925
35	25.46504707055973	23.947514020571887	25.968655953189312	24.618782955679062
36	23.57426082874364	24.992874447769704	25.710878622505007	25.72198610098165
37	25.07104594723738	23.6048587883208	25.92506433954514	25.39903092489668
38	23.965956626344422	22.87029818340333	27.204520115014795	25.95922507523745
39	24.874883685838594	22.70578175690969	26.424691295928376	25.99464326132334
40	23.750094308779516	23.00086344927026	27.278290538104937	25.970751703845284
41	22.43563949735516	23.302022818533143	28.462180083662364	25.80015760044933
42	23.986914132904126	22.854789628549156	28.05434700601061	25.10394923253611
43	22.237800635431597	22.26630284435279	28.711155261591596	26.784741258624013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	42.0
2	46.0
3	175.0
4	304.0
5	304.0
6	409.0
7	514.0
8	487.0
9	460.0
10	692.5
11	925.0
12	925.0
13	1667.5
14	2410.0
15	3858.0
16	5306.0
17	4728.5
18	4151.0
19	4151.0
20	4628.0
21	5105.0
22	4021.0
23	2937.0
24	3104.5
25	3272.0
26	3272.0
27	3723.5
28	4175.0
29	5054.0
30	5933.0
31	7063.0
32	8193.0
33	8193.0
34	9824.0
35	11455.0
36	13496.5
37	15538.0
38	17849.0
39	20160.0
40	20160.0
41	21715.0
42	23270.0
43	24022.0
44	24774.0
45	26756.0
46	28738.0
47	28738.0
48	32253.0
49	35768.0
50	36505.5
51	37243.0
52	37542.0
53	37841.0
54	37841.0
55	35969.0
56	34097.0
57	32715.5
58	31334.0
59	30350.5
60	29367.0
61	29367.0
62	27645.5
63	25924.0
64	24024.0
65	22124.0
66	20021.5
67	17919.0
68	17919.0
69	15309.0
70	12699.0
71	11258.5
72	9818.0
73	7602.5
74	5387.0
75	5387.0
76	4269.0
77	3151.0
78	2690.0
79	2229.0
80	1916.5
81	1604.0
82	1604.0
83	1314.5
84	1025.0
85	955.0
86	885.0
87	782.5
88	680.0
89	680.0
90	493.5
91	307.0
92	172.0
93	37.0
94	22.5
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	477156.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.029292908837775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.02645345873914	48.10036330557231
2	5.468740690911453	5.909443852634695
3	1.6232374126169873	2.631071088806015
4	0.8366122743221652	1.808062784819248
5	0.4983179410650297	1.3461882999765724
6	0.3662238542811947	1.1872089535897314
7	0.26342017771779097	0.9962684158008841
8	0.20321995604879656	0.8783864424225254
9	0.15678430833221665	0.7623850786551781
>10	1.2501684173050842	13.589284758415943
>50	0.173606600472253	6.640592298581434
>100	0.12738918334000554	12.958450887830915
>500	0.004660579878292885	1.701801287371
>1k	0.0011651449695732213	1.4904925455235774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2813	0.5895346595243484	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2296	0.4811843506107017	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1994	0.4178926808004091	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	885	0.18547393305334106	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	836	0.17520475483908826	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	819	0.17164197872393933	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	690	0.1446067952619269	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	681	0.14272061967155394	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	661	0.13852911835961407	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	655	0.13727166796603207	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	639	0.13391846691648016	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	632	0.13245144145730117	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	595	0.12469716403021233	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	516	0.1081407338480497	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	501	0.10499710786409475	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	498	0.10436838266730376	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	498	0.10436838266730376	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	485	0.10164390681454284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.0957506559699554E-4	0.0
13	0.0	0.0	0.0	2.0957506559699554E-4	0.0
14	0.0	0.0	0.0	2.0957506559699554E-4	0.0
15	0.0	0.0	0.0	4.191501311939911E-4	0.0
16	0.0	0.0	0.0	0.0012574503935819732	0.0
17	0.0	0.0	0.0	0.002724475852760942	0.0
18	0.0	0.0	0.0	0.0029340509183579376	0.0
19	0.0	0.0	0.0	0.003143625983954933	0.0
20	0.0	0.0	0.0	0.003981926246342915	0.0
21	0.0	0.0	0.0	0.005029801574327893	0.0
22	0.0	0.0	0.0	0.007125552230297848	0.0
23	0.0	0.0	0.0	0.011317053542237759	0.0
24	0.0	0.0	0.0	0.014879829657386682	0.0
25	0.0	0.0	0.0	0.01781388057574462	0.0
26	0.0	0.0	0.0	0.02389155747805749	0.0
27	0.0	0.0	0.0	0.045896939365742025	0.0
28	0.0	0.0	0.0	0.12448758896461534	0.0
29	0.0	0.0	0.0	0.23640067399341097	0.0
30	0.0	0.0	0.0	0.3812170443209349	0.0
31	0.0	0.0	0.0	0.6968370931100102	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	25	0.0054931613	29.6	4
CGCCTAT	40	5.93296E-5	27.750002	36
TCTAATA	50	9.076544E-6	25.900002	2
CTATTGC	50	9.076544E-6	25.900002	10
GTACTAT	75	1.3695171E-8	24.666666	1
ATAGTAC	45	1.3216282E-4	24.666666	3
TCTATCG	45	1.3216282E-4	24.666666	31
AAGACGG	165	0.0	23.545456	5
GTTCATA	40	0.0019295409	23.125002	1
AGAGCGA	170	0.0	22.85294	15
CGCAAGA	180	0.0	22.61111	2
TATCGCC	50	2.6988934E-4	22.2	33
CAAGACG	210	0.0	22.02381	4
CGGACCA	175	0.0	21.142857	9
CAGAGCG	195	0.0	20.871796	14
CGTATGC	45	0.0038225786	20.555555	11
TTATGGG	45	0.0038225786	20.555555	2
ATGCTAG	45	0.0038225786	20.555555	15
TATACAG	45	0.0038225786	20.555555	5
AGACGGA	190	0.0	20.447369	6
>>END_MODULE