FastQCFastQC Report
Fri 10 Feb 2017
ERR1631408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23781
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT640.26912240864555736TruSeq Adapter, Index 8 (95% over 22bp)
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT270.11353601614734453No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC250.10512594087717085No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG240.10092090324208401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352.2920347E-531.7142871
GACTGTC250.005389986629.59999837
GTATCAA451.2707117E-424.6666662
TCTTATA450.00371821520.55555537
TCAACGC608.879023E-418.55
TATCAAC608.879023E-418.53
ATCAACG608.879023E-418.54
CAACGCA608.879023E-418.56
AACGCAG650.001519226717.0769237
CAGAGTA700.00249368315.85714311
AGAGTAC700.00249368315.85714312
CGCAGAG700.00249368315.8571439
CTCTTAT750.003949238514.837
AGTACAT750.003949238514.814
GAGTACA750.003949238514.813
GCAGAGT750.003949238514.810
ACATGGG800.00606243713.87500117
ACGCAGA800.00606243713.8750018
TACATGG800.00606243713.87500116
TCTCTTA1501.1585325E-613.56666737
Produced by FastQC (version 0.11.5)