##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631408.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23781
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95525839956267	34.0	33.0	34.0	31.0	34.0
2	33.09923888818805	34.0	33.0	34.0	31.0	34.0
3	33.17143938438249	34.0	33.0	34.0	31.0	34.0
4	36.459989066902146	37.0	37.0	37.0	35.0	37.0
5	36.45128463899752	37.0	37.0	37.0	35.0	37.0
6	36.50199739287667	37.0	37.0	37.0	35.0	37.0
7	36.50637063201716	37.0	37.0	37.0	35.0	37.0
8	36.503637357554354	37.0	37.0	37.0	35.0	37.0
9	38.33602455741979	39.0	39.0	39.0	37.0	39.0
10	38.28274673058324	39.0	39.0	39.0	37.0	39.0
11	38.33409024010765	39.0	39.0	39.0	37.0	39.0
12	38.29149320886422	39.0	39.0	39.0	37.0	39.0
13	38.33097851225769	39.0	39.0	39.0	37.0	39.0
14	39.83402716454312	41.0	40.0	41.0	38.0	41.0
15	39.81783776964804	41.0	40.0	41.0	38.0	41.0
16	39.78873890921324	41.0	40.0	41.0	38.0	41.0
17	39.753374542702154	41.0	40.0	41.0	38.0	41.0
18	39.76830242630672	41.0	40.0	41.0	38.0	41.0
19	39.765527101467555	41.0	40.0	41.0	38.0	41.0
20	39.757789832219	41.0	40.0	41.0	38.0	41.0
21	39.752323283293386	41.0	40.0	41.0	38.0	41.0
22	39.70169463016694	41.0	40.0	41.0	38.0	41.0
23	39.64955216349186	41.0	40.0	41.0	37.0	41.0
24	39.65169673268576	41.0	40.0	41.0	37.0	41.0
25	39.58773811025608	41.0	40.0	41.0	37.0	41.0
26	39.513182792985994	41.0	39.0	41.0	37.0	41.0
27	39.448593414911066	41.0	39.0	41.0	37.0	41.0
28	39.42319498759514	41.0	39.0	41.0	37.0	41.0
29	39.36524956898364	41.0	39.0	41.0	36.0	41.0
30	39.26092258525714	41.0	39.0	41.0	36.0	41.0
31	39.201715655355116	41.0	39.0	41.0	36.0	41.0
32	39.13531811109709	41.0	39.0	41.0	35.0	41.0
33	39.086918127917244	41.0	39.0	41.0	35.0	41.0
34	39.05521214414869	40.0	39.0	41.0	35.0	41.0
35	38.93890080316219	40.0	39.0	41.0	35.0	41.0
36	38.86955973255961	40.0	39.0	41.0	35.0	41.0
37	38.80816618308734	40.0	38.0	41.0	35.0	41.0
38	38.74042302678609	40.0	38.0	41.0	35.0	41.0
39	38.66876918548421	40.0	38.0	41.0	35.0	41.0
40	38.597031243429626	40.0	38.0	41.0	35.0	41.0
41	38.506076279382704	40.0	38.0	41.0	35.0	41.0
42	38.39666120011774	40.0	38.0	41.0	35.0	41.0
43	37.60720743450654	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	4.0
21	4.0
22	6.0
23	12.0
24	16.0
25	14.0
26	33.0
27	45.0
28	73.0
29	82.0
30	129.0
31	150.0
32	213.0
33	278.0
34	447.0
35	662.0
36	943.0
37	2036.0
38	5382.0
39	13251.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.934233211387244	18.590471384718892	12.173583953576385	25.301711450317484
2	21.03780328833943	18.758672890122366	32.98431520962112	27.21920861191708
3	19.14133131491527	21.719019385223497	30.036583827425257	29.10306547243598
4	14.545225179765358	16.929481518859593	35.002733274462805	33.52256002691224
5	15.546024136916026	35.22139523148732	34.30890206467348	14.923678566923174
6	33.77906732265254	35.48631260249779	15.596484588537068	15.138135486312605
7	31.167738951263612	28.787687649804468	20.856986670030697	19.187586728901223
8	28.186367267987052	32.08864219334763	18.712417476136412	21.01257306252891
9	27.551406585088937	13.657962238762037	18.674572137420633	40.116059038728395
10	20.07905470753963	23.157142256423192	30.29309112316555	26.470711912871618
11	37.50473066733947	21.348976073335855	19.898238089230897	21.24805517009377
12	24.27988730499138	25.29750641268239	25.125099869643837	25.29750641268239
13	32.130692569698496	18.060636642697954	22.690383078928555	27.11828770867499
14	23.657541734998528	19.05723056221353	23.169757369328455	34.115470333459484
15	28.99793953155881	24.09486564904756	20.339767040915017	26.56742777847862
16	28.232622681973	23.737437450065176	21.67696900887263	26.352970859089186
17	24.990538665321054	25.427862579370085	22.509566460619823	27.072032294689034
18	24.881207686808796	23.11088684243724	24.11589083722299	27.89201463353097
19	27.643917413060848	23.54400571885118	24.34296286951768	24.46911399857029
20	26.390816197804973	23.228627896219674	24.813927084647407	25.566628821327953
21	27.913039821706402	22.74925360581977	22.690383078928555	26.647323493545265
22	28.304108321769476	22.736638492914512	22.16895841217779	26.790294773138218
23	26.495942138682143	23.632311509188007	23.901433917833565	25.97031243429629
24	26.420251461250576	23.09827172953198	22.602077288591733	27.879399520625707
25	27.05941718178378	22.68197300365838	23.375804213447708	26.88280560111013
26	26.777679660232963	23.817333165131828	23.939279256549344	25.46570791808587
27	26.31092048273832	24.048610235061606	22.23623901433918	27.404230267860896
28	25.94087717085068	22.984735713384634	25.251250998696438	25.823136117068245
29	27.36217989151003	23.119296917707413	24.725621294310585	24.79290189647197
30	26.40763634834532	22.745048568184686	24.578444977082544	26.268870106387453
31	26.260460031117276	23.438879777974012	23.834153315672175	26.466506875236533
32	24.616290315798324	23.527185568310838	24.99474370295614	26.861780412934692
33	24.485934149110637	23.304318573651233	25.331146713763086	26.87860056347504
34	26.260460031117276	21.685379084142802	26.231024767671666	25.823136117068245
35	25.953492283755942	23.064631428451285	25.772675665447203	25.209200622345566
36	25.188175434170134	22.307724654135654	25.566628821327953	26.93747109036626
37	24.380808208233464	20.65514486354653	26.971111391446957	27.992935536773057
38	23.93507421891426	20.423867793616754	28.585845843320296	27.055212144148687
39	23.152937218788107	19.767881922543207	29.729616080063913	27.34956477860477
40	22.68617804129347	19.902443126865986	31.361170682477606	26.050208149362934
41	21.424666750767418	19.58706530423447	31.38219587065304	27.606072074345068
42	20.482738320507966	20.373407341995712	31.966696101930115	27.177158235566207
43	20.1211050838905	19.280097556873134	32.883394306379046	27.715403052857322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	1.5
7	3.0
8	2.0
9	1.0
10	3.0
11	5.0
12	5.0
13	8.0
14	11.0
15	16.5
16	22.0
17	20.5
18	19.0
19	19.0
20	21.5
21	24.0
22	18.0
23	12.0
24	16.0
25	20.0
26	20.0
27	27.5
28	35.0
29	80.5
30	126.0
31	171.5
32	217.0
33	217.0
34	333.0
35	449.0
36	603.0
37	757.0
38	891.0
39	1025.0
40	1025.0
41	1240.0
42	1455.0
43	1645.5
44	1836.0
45	1981.0
46	2126.0
47	2126.0
48	2206.5
49	2287.0
50	2329.5
51	2372.0
52	2283.5
53	2195.0
54	2195.0
55	2057.0
56	1919.0
57	1673.0
58	1427.0
59	1331.5
60	1236.0
61	1236.0
62	1120.5
63	1005.0
64	959.5
65	914.0
66	820.0
67	726.0
68	726.0
69	640.0
70	554.0
71	480.5
72	407.0
73	330.0
74	253.0
75	253.0
76	206.0
77	159.0
78	123.5
79	88.0
80	67.5
81	47.0
82	47.0
83	35.0
84	23.0
85	19.0
86	15.0
87	11.5
88	8.0
89	8.0
90	4.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23781.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.90273747950044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4609794082021	61.57436609057652
2	8.398223452731154	12.245069593372861
3	2.9647574551537175	6.484168033303899
4	1.6035069504527888	4.67600185021656
5	0.73253734786872	2.6701988982801397
6	0.5075849339562785	2.2202598713258483
7	0.3460806367883717	1.7661158067364704
8	0.2710964988175578	1.5810941507926495
9	0.16727230778104632	1.0975148227576637
>10	0.5421929976351156	5.4160884739918425
>50	0.005768010613139528	0.26912240864555736
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	64	0.26912240864555736	TruSeq Adapter, Index 8 (95% over 22bp)
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	27	0.11353601614734453	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	25	0.10512594087717085	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG	24	0.10092090324208401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.004205037635086834	0.0
25	0.0	0.0	0.0	0.004205037635086834	0.0
26	0.0	0.0	0.0	0.012615112905260501	0.0
27	0.0	0.0	0.0	0.08830579033682351	0.0
28	0.0	0.0	0.0	0.2228669946596022	0.0
29	0.0	0.0	0.0	0.4415289516841176	0.0
30	0.0	0.0	0.0	0.7569067743156301	0.0
31	0.0	0.0	0.0	1.8460115218031201	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	2.2920347E-5	31.714287	1
GACTGTC	25	0.0053899866	29.599998	37
GTATCAA	45	1.2707117E-4	24.666666	2
TCTTATA	45	0.003718215	20.555555	37
TCAACGC	60	8.879023E-4	18.5	5
TATCAAC	60	8.879023E-4	18.5	3
ATCAACG	60	8.879023E-4	18.5	4
CAACGCA	60	8.879023E-4	18.5	6
AACGCAG	65	0.0015192267	17.076923	7
CAGAGTA	70	0.002493683	15.857143	11
AGAGTAC	70	0.002493683	15.857143	12
CGCAGAG	70	0.002493683	15.857143	9
CTCTTAT	75	0.0039492385	14.8	37
AGTACAT	75	0.0039492385	14.8	14
GAGTACA	75	0.0039492385	14.8	13
GCAGAGT	75	0.0039492385	14.8	10
ACATGGG	80	0.006062437	13.875001	17
ACGCAGA	80	0.006062437	13.875001	8
TACATGG	80	0.006062437	13.875001	16
TCTCTTA	150	1.1585325E-6	13.566667	37
>>END_MODULE