FastQCFastQC Report
Fri 10 Feb 2017
ERR1631407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24827
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.8015467031860475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1430.5759858218874613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1430.5759858218874613No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1300.5236234744431465No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1180.47528899987916384No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1180.47528899987916384No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1040.4188987795545172No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT970.390703669392194No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT790.3182019575462198No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC750.30209046602489226No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA710.2859789745035647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.27389535586256897No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA670.26986748298223706No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT650.26181173722157325TruSeq Adapter, Index 6 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA630.2537559914609095No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA600.2416723728199138TruSeq Adapter, Index 25 (96% over 27bp)
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA520.2094493897772586No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT520.2094493897772586No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC450.18125427961493537No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC440.17722640673460346No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC440.17722640673460346No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT430.17319853385427156No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT420.16917066097393965No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA400.16111491521327587No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT390.15708704233294396No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT380.15305916945261208No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT380.15305916945261208No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT380.15305916945261208No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT370.14903129657228018No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA360.14500342369194827No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA350.1409755508116164No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT340.13694767793128448No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT340.13694767793128448No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA330.1329198050509526No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA330.1329198050509526No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT330.1329198050509526No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA310.12486405929028879No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC310.12486405929028879No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC310.12486405929028879No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT300.1208361864099569No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATA300.1208361864099569No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT300.1208361864099569No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT290.116808313529625No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT290.116808313529625No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTT290.116808313529625No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG290.116808313529625No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA290.116808313529625No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA280.11278044064929311No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT280.11278044064929311No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT280.11278044064929311No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT280.11278044064929311No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.10875256776896121No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA260.1047246948886293No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATAC260.1047246948886293No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT250.10069682200829742No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA250.10069682200829742No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGCTGTCTCT250.10069682200829742No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT250.10069682200829742No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC250.10069682200829742No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT250.10069682200829742No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG200.001807433437.06
AAATCTC200.001807433437.05
GAAATCT200.001807433437.04
TTCTGCT200.001807433437.022
AGAAATC200.001807433437.03
GTCTTCT200.001807433437.019
CTGCTTG200.001807433437.024
TTGAAAA200.001807433437.028
CAGAAAT200.001807433437.02
CTTGAAA200.001807433437.027
TACCATG200.001807433437.07
CGTCTTC200.001807433437.018
TGAAAAA200.001807433437.029
AGTGGCT401.518185E-632.37527
ATACCAT352.2961347E-531.7142856
TGCTTGA250.005394553829.625
GCAGAAA250.005394553829.61
GCTTGAA250.005394553829.626
GCCGTCT250.005394553829.616
CTTCTGC250.005394553829.621
Produced by FastQC (version 0.11.5)