##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24827
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.819591573689934	34.0	31.0	34.0	31.0	34.0
2	32.92455794095138	34.0	33.0	34.0	31.0	34.0
3	32.91029927095501	34.0	33.0	34.0	31.0	34.0
4	36.27554678374351	37.0	37.0	37.0	35.0	37.0
5	36.28908043662142	37.0	37.0	37.0	35.0	37.0
6	36.367865630160715	37.0	37.0	37.0	35.0	37.0
7	36.388327224392796	37.0	37.0	37.0	35.0	37.0
8	36.41418616828453	37.0	37.0	37.0	35.0	37.0
9	38.237040319007534	39.0	39.0	39.0	37.0	39.0
10	38.19796995206831	39.0	39.0	39.0	37.0	39.0
11	38.235590284770616	39.0	39.0	39.0	37.0	39.0
12	38.204213155032825	39.0	39.0	39.0	37.0	39.0
13	38.21037580053974	39.0	39.0	39.0	37.0	39.0
14	39.57429411527772	41.0	39.0	41.0	37.0	41.0
15	39.55669231079067	41.0	39.0	41.0	37.0	41.0
16	39.42812260845048	41.0	39.0	41.0	37.0	41.0
17	39.40822491642164	41.0	39.0	41.0	37.0	41.0
18	39.58774721069803	41.0	39.0	41.0	37.0	41.0
19	39.63527611068594	41.0	40.0	41.0	37.0	41.0
20	39.64889032102147	41.0	40.0	41.0	37.0	41.0
21	39.67487010109961	41.0	40.0	41.0	37.0	41.0
22	39.583799895275305	41.0	40.0	41.0	37.0	41.0
23	39.54549482418335	41.0	40.0	41.0	37.0	41.0
24	39.48394892657188	41.0	39.0	41.0	37.0	41.0
25	39.44499939581907	41.0	39.0	41.0	36.0	41.0
26	39.35755427558706	41.0	39.0	41.0	36.0	41.0
27	39.25500463205381	41.0	39.0	41.0	36.0	41.0
28	39.17646111088734	41.0	39.0	41.0	35.0	41.0
29	39.083900592097315	41.0	39.0	41.0	35.0	41.0
30	38.92185926612156	40.0	38.0	41.0	35.0	41.0
31	38.759979055061024	40.0	38.0	41.0	35.0	41.0
32	38.67144640915133	40.0	38.0	41.0	35.0	41.0
33	38.56217021790792	40.0	38.0	41.0	35.0	41.0
34	38.46908607564345	40.0	38.0	41.0	35.0	41.0
35	38.35344584524913	40.0	38.0	41.0	35.0	41.0
36	38.28473033391066	40.0	38.0	41.0	35.0	41.0
37	38.12683771700165	40.0	38.0	41.0	34.0	41.0
38	37.9733354815322	40.0	37.0	41.0	34.0	41.0
39	37.7839851774278	40.0	37.0	41.0	34.0	41.0
40	37.63249687839852	40.0	37.0	41.0	33.0	41.0
41	37.463648447255004	40.0	36.0	41.0	33.0	41.0
42	37.300318201957545	40.0	36.0	41.0	33.0	41.0
43	36.515366335038465	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	0.0
19	2.0
20	1.0
21	5.0
22	7.0
23	21.0
24	25.0
25	46.0
26	55.0
27	76.0
28	139.0
29	192.0
30	232.0
31	273.0
32	371.0
33	417.0
34	559.0
35	808.0
36	1209.0
37	2627.0
38	4883.0
39	12877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14254642123495	13.803520360897409	17.13457123293189	25.919361984935758
2	20.50992870665002	19.64393603737866	34.079832440488175	25.766302815483144
3	17.762919402263663	23.832923832923832	31.638941475007048	26.765215289805454
4	13.775325250735088	19.438514520481736	31.792000644459662	34.99415958432352
5	14.266725742135577	38.1359004309824	30.623917509163412	16.973456317718615
6	27.590929230273492	33.06078060176421	19.950054376283884	19.398235791678413
7	28.803318967253393	32.54924074596205	17.940145808998267	20.70729447778628
8	32.96813952551658	28.94429451806501	18.657107181697345	19.43045877472107
9	29.725701856849398	12.442099327345229	15.475087606235146	42.35711120957023
10	21.315503282716396	24.195432392153705	23.172352680549402	31.316711644580497
11	33.20981189833649	25.01711845974141	14.701736013211422	27.071333628710676
12	24.90433801909212	30.49099770411246	23.34152334152334	21.26314093527208
13	34.70415273693962	18.069037741168888	25.085592298707056	22.141217223184437
14	24.07459620574375	21.79079228259556	23.20860353647239	30.926007975188302
15	32.6297982035687	23.063600112780442	20.715350223546945	23.59125146010392
16	25.162121883433358	25.50046320538124	24.598219680186894	24.739195230998508
17	18.7779433681073	31.24018205985419	23.6516695533089	26.330205018729608
18	24.04237322270109	21.29939179119507	26.47923631530189	28.178998670801946
19	24.57002457002457	29.310830950175216	25.814637290047127	20.30450718975309
20	27.329117493051918	21.08591452853748	29.633060780601767	21.951907197808836
21	32.99230676279857	19.490876867926048	24.81975268860515	22.697063680670237
22	30.362105771941838	21.150360494622788	21.40814435896403	27.07938937447134
23	25.29504168848431	24.525717968340917	22.749426028114552	27.42981431506022
24	23.808756595641842	24.199460265034038	27.554678374350505	24.437104764973615
25	22.70109155355057	23.42208079912998	25.90727836629476	27.96954928102469
26	29.94320699238732	21.855238248680873	22.78970475691787	25.411850002013935
27	25.31115318000564	22.3385829943207	22.689007934909576	29.66125589076409
28	22.781649011157207	22.048576146936803	28.81137471301406	26.358400128891933
29	24.01417811253877	22.193579570628753	30.3701615177025	23.42208079912998
30	24.231683248076692	23.236798646634714	29.96334635678898	22.56817174849962
31	22.938736053490153	25.59310428162887	25.858943891730778	25.6092157731502
32	21.802875901236558	24.586136061545897	31.04684416159826	22.564143875619287
33	22.84609497724252	22.797760502678535	32.06589600032223	22.290248519756716
34	24.36460305312764	24.461272002255612	25.7985257985258	25.37559914609095
35	21.460506706408346	24.40488178193096	27.9453820437427	26.18922946791799
36	18.14153945301486	22.858178595883512	29.302775204414548	29.697506746687075
37	19.591573689934346	22.713175172191566	31.79602851733999	25.899222620534097
38	20.07089056269384	18.677246546099006	33.1614774237725	28.090385467434647
39	19.70838200346397	18.89475168163693	36.44822169412333	24.948644620775767
40	18.8786401901156	18.959197647722238	33.757602610061625	28.404559552100533
41	14.979659241954323	22.2378861723124	36.92753856688283	25.854916018850442
42	14.601039191203125	20.586458291376324	35.59028477061264	29.22221774680791
43	13.122809844121319	24.114874934547064	36.61739235509727	26.144922866234342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	9.5
4	16.0
5	16.0
6	28.0
7	40.0
8	38.0
9	36.0
10	72.5
11	109.0
12	109.0
13	160.5
14	212.0
15	325.5
16	439.0
17	392.0
18	345.0
19	345.0
20	350.0
21	355.0
22	246.0
23	137.0
24	140.0
25	143.0
26	143.0
27	139.5
28	136.0
29	149.5
30	163.0
31	188.5
32	214.0
33	214.0
34	277.5
35	341.0
36	370.0
37	399.0
38	474.5
39	550.0
40	550.0
41	703.0
42	856.0
43	1198.0
44	1540.0
45	2122.5
46	2705.0
47	2705.0
48	3171.5
49	3638.0
50	3335.5
51	3033.0
52	2484.5
53	1936.0
54	1936.0
55	1631.5
56	1327.0
57	1263.5
58	1200.0
59	1119.0
60	1038.0
61	1038.0
62	973.5
63	909.0
64	869.5
65	830.0
66	750.5
67	671.0
68	671.0
69	609.5
70	548.0
71	477.0
72	406.0
73	323.5
74	241.0
75	241.0
76	187.5
77	134.0
78	117.0
79	100.0
80	71.0
81	42.0
82	42.0
83	30.5
84	19.0
85	13.5
86	8.0
87	5.5
88	3.0
89	3.0
90	3.0
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	24827.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.06670157489829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.30337581041806	65.79933137310186
2	4.594232059020791	6.621823015265638
3	1.4419852448021462	3.117573609376888
4	0.6483344511513526	1.868933016474
5	0.4303599374021909	1.55073105892778
6	0.3241672255756763	1.4016997623555
7	0.19561815336463223	0.9868288556813147
8	0.1676727028839705	0.9666894912796552
9	0.08383635144198524	0.5437628388448061
>10	0.7098144422088084	10.279131590607001
>50	0.06147999105745584	3.0168767873685907
>100	0.03912363067292645	3.846618600716961
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.8015467031860475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	143	0.5759858218874613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	143	0.5759858218874613	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	130	0.5236234744431465	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	118	0.47528899987916384	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	118	0.47528899987916384	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	104	0.4188987795545172	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	97	0.390703669392194	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	79	0.3182019575462198	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	75	0.30209046602489226	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	71	0.2859789745035647	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.27389535586256897	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	67	0.26986748298223706	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	65	0.26181173722157325	TruSeq Adapter, Index 6 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	63	0.2537559914609095	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	60	0.2416723728199138	TruSeq Adapter, Index 25 (96% over 27bp)
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	52	0.2094493897772586	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	52	0.2094493897772586	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	45	0.18125427961493537	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	44	0.17722640673460346	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	44	0.17722640673460346	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	43	0.17319853385427156	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	42	0.16917066097393965	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	40	0.16111491521327587	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	39	0.15708704233294396	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	38	0.15305916945261208	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	38	0.15305916945261208	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	38	0.15305916945261208	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	37	0.14903129657228018	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA	36	0.14500342369194827	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	35	0.1409755508116164	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	34	0.13694767793128448	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT	34	0.13694767793128448	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	33	0.1329198050509526	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	33	0.1329198050509526	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	33	0.1329198050509526	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	31	0.12486405929028879	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	31	0.12486405929028879	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	31	0.12486405929028879	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	30	0.1208361864099569	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATA	30	0.1208361864099569	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	30	0.1208361864099569	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT	29	0.116808313529625	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	29	0.116808313529625	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTT	29	0.116808313529625	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	29	0.116808313529625	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	29	0.116808313529625	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	28	0.11278044064929311	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	28	0.11278044064929311	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	28	0.11278044064929311	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	28	0.11278044064929311	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.10875256776896121	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.1047246948886293	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATAC	26	0.1047246948886293	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	25	0.10069682200829742	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA	25	0.10069682200829742	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGCTGTCTCT	25	0.10069682200829742	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT	25	0.10069682200829742	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	25	0.10069682200829742	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	25	0.10069682200829742	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008055745760663794	0.0
2	0.0	0.0	0.0	0.008055745760663794	0.0
3	0.0	0.0	0.0	0.008055745760663794	0.0
4	0.0	0.0	0.0	0.008055745760663794	0.0
5	0.0	0.0	0.0	0.008055745760663794	0.0
6	0.0	0.0	0.0	0.008055745760663794	0.0
7	0.0	0.0	0.0	0.008055745760663794	0.0
8	0.0	0.0	0.0	0.008055745760663794	0.0
9	0.0	0.0	0.0	0.008055745760663794	0.0
10	0.0	0.0	0.0	0.008055745760663794	0.0
11	0.0	0.0	0.0	0.008055745760663794	0.0
12	0.0	0.0	0.0	0.008055745760663794	0.0
13	0.0	0.0	0.0	0.008055745760663794	0.0
14	0.0	0.0	0.0	0.008055745760663794	0.0
15	0.0	0.0	0.0	0.008055745760663794	0.0
16	0.0	0.0	0.0	0.008055745760663794	0.0
17	0.0	0.0	0.0	0.020139364401659483	0.0
18	0.0	0.0	0.0	0.02416723728199138	0.0
19	0.0	0.0	0.0	0.02416723728199138	0.0
20	0.0	0.0	0.0	0.02416723728199138	0.0
21	0.0	0.0	0.0	0.03625085592298707	0.0
22	0.0	0.0	0.0	0.04027872880331897	0.0
23	0.0	0.0	0.0	0.08861320336730173	0.0
24	0.0	0.0	0.0	0.19736577113626294	0.0
25	0.0	0.0	0.0	0.22556088129858623	0.0
26	0.0	0.0	0.0	0.2618117372215733	0.0
27	0.0	0.0	0.0	0.40681516091352155	0.0
28	0.0	0.0	0.0	0.8659926692713578	0.0
29	0.0	0.0	0.0	1.7279574656623837	0.0
30	0.0	0.0	0.0	3.2102146856245217	0.0
31	0.0	0.0	0.0	5.824304184959923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTCG	20	0.0018074334	37.0	6
AAATCTC	20	0.0018074334	37.0	5
GAAATCT	20	0.0018074334	37.0	4
TTCTGCT	20	0.0018074334	37.0	22
AGAAATC	20	0.0018074334	37.0	3
GTCTTCT	20	0.0018074334	37.0	19
CTGCTTG	20	0.0018074334	37.0	24
TTGAAAA	20	0.0018074334	37.0	28
CAGAAAT	20	0.0018074334	37.0	2
CTTGAAA	20	0.0018074334	37.0	27
TACCATG	20	0.0018074334	37.0	7
CGTCTTC	20	0.0018074334	37.0	18
TGAAAAA	20	0.0018074334	37.0	29
AGTGGCT	40	1.518185E-6	32.375	27
ATACCAT	35	2.2961347E-5	31.714285	6
TGCTTGA	25	0.0053945538	29.6	25
GCAGAAA	25	0.0053945538	29.6	1
GCTTGAA	25	0.0053945538	29.6	26
GCCGTCT	25	0.0053945538	29.6	16
CTTCTGC	25	0.0053945538	29.6	21
>>END_MODULE