FastQCFastQC Report
Fri 10 Feb 2017
ERR1631406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429853
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32440.7546765987442219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28930.6730207768702324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24190.5627505216899731No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.2793978406571549No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4820.11213135653351262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC200.001840654437.011
ATAACGA303.5957634E-430.83333212
TATGCCG250.005492564329.59999813
AGCGTCA706.5683707E-926.4285723
GCAGCGT856.91216E-1126.1176471
TAACGAA400.001929231223.12500213
TTGCGTT400.001929231223.1250024
CAGCGTC1059.767973E-1021.1428572
GATAACG450.003821970220.55555711
CTATAGT450.003821970220.5555574
TTTGACG450.003821970220.55555731
ACGAACG450.003821970220.55555715
GCGTCAG951.6700324E-719.4736844
TTAATTC902.1458436E-618.53
CGAAAGC801.6128366E-518.519
CGATAAC609.223998E-418.510
CGAACGA500.00702789518.49999816
TATGGGT500.00702789518.4999983
AACGAAC500.00702789518.49999814
ATACCGC1002.8645263E-718.49999827
Produced by FastQC (version 0.11.5)