##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631406.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429853
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01437933433057	34.0	33.0	34.0	31.0	34.0
2	33.13388297859966	34.0	33.0	34.0	31.0	34.0
3	33.20473510711801	34.0	33.0	34.0	31.0	34.0
4	36.44571051033726	37.0	37.0	37.0	35.0	37.0
5	36.43012843925714	37.0	37.0	37.0	35.0	37.0
6	36.49026062398076	37.0	37.0	37.0	35.0	37.0
7	36.503993225591074	37.0	37.0	37.0	35.0	37.0
8	36.514280463321185	37.0	37.0	37.0	35.0	37.0
9	38.3537558188497	39.0	39.0	39.0	37.0	39.0
10	38.318632183560425	39.0	39.0	39.0	37.0	39.0
11	38.37273207352281	39.0	39.0	39.0	37.0	39.0
12	38.339385790025894	39.0	39.0	39.0	37.0	39.0
13	38.36655786978339	39.0	39.0	39.0	37.0	39.0
14	39.87918660565356	41.0	40.0	41.0	38.0	41.0
15	39.84879016780155	41.0	40.0	41.0	38.0	41.0
16	39.8006109065192	41.0	40.0	41.0	38.0	41.0
17	39.81958716119232	41.0	40.0	41.0	38.0	41.0
18	39.80344210695284	41.0	40.0	41.0	38.0	41.0
19	39.81908931658032	41.0	40.0	41.0	38.0	41.0
20	39.783593461020395	41.0	40.0	41.0	38.0	41.0
21	39.753006260279676	41.0	40.0	41.0	38.0	41.0
22	39.674726010985154	41.0	40.0	41.0	37.0	41.0
23	39.607938062546964	41.0	40.0	41.0	37.0	41.0
24	39.573607721709514	41.0	40.0	41.0	37.0	41.0
25	39.57271671943664	41.0	40.0	41.0	37.0	41.0
26	39.443305036838176	41.0	39.0	41.0	36.0	41.0
27	39.37640076956541	41.0	39.0	41.0	36.0	41.0
28	39.30353399883216	41.0	39.0	41.0	35.0	41.0
29	39.227454501887856	41.0	39.0	41.0	35.0	41.0
30	39.13893121602036	41.0	39.0	41.0	35.0	41.0
31	39.044098796565336	41.0	39.0	41.0	35.0	41.0
32	38.93600602996838	41.0	38.0	41.0	35.0	41.0
33	38.832533447480884	41.0	38.0	41.0	35.0	41.0
34	38.741358092185	40.0	38.0	41.0	35.0	41.0
35	38.63010843241759	40.0	38.0	41.0	35.0	41.0
36	38.51809106834197	40.0	38.0	41.0	35.0	41.0
37	38.42207452315094	40.0	38.0	41.0	35.0	41.0
38	38.30019099552638	40.0	38.0	41.0	35.0	41.0
39	38.15943590017983	40.0	37.0	41.0	35.0	41.0
40	38.007921312634785	40.0	37.0	41.0	34.0	41.0
41	37.850595436114205	40.0	37.0	41.0	34.0	41.0
42	37.65864842166973	40.0	37.0	41.0	33.0	41.0
43	36.85205407429982	40.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	1.0
13	0.0
14	1.0
15	2.0
16	5.0
17	4.0
18	9.0
19	14.0
20	32.0
21	53.0
22	138.0
23	181.0
24	322.0
25	537.0
26	749.0
27	1181.0
28	1683.0
29	2419.0
30	3189.0
31	3908.0
32	4882.0
33	6426.0
34	9007.0
35	12675.0
36	19564.0
37	41000.0
38	78180.0
39	243685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01900184481672	17.47574170704869	12.718068735125728	25.787187713008862
2	19.683007912007128	20.111293860924548	33.16156918760599	27.044129039462327
3	20.856664952902506	22.03520738485017	28.317587640425913	28.790540021821414
4	14.946737605646582	15.790281794008648	32.04095353527834	37.222027065066435
5	16.189720671950646	35.45467869248324	31.315589282847856	17.040011352718253
6	34.61578725750431	32.31825763691308	16.383507850358146	16.68244725522446
7	30.447618139224343	29.714809481380843	19.829802281244984	20.007770098149834
8	29.672236787925176	30.63605465124124	19.04604597385618	20.64566258697741
9	28.088672173975755	13.794715867982777	19.282173208050192	38.83443874999127
10	19.526210122995536	25.488248308142552	29.770875159647602	25.214666409214313
11	36.43594438098606	21.785354528175912	19.449439692173836	22.329261398664194
12	23.35216922994605	25.79812168345923	26.90803600300568	23.94167308358904
13	31.73061488462335	18.605430228473455	24.3241294116826	25.3398254752206
14	23.92027041802663	20.33346283496916	24.38112564062598	31.365141106378225
15	27.388432789814193	25.96911037028938	21.736267979983857	24.906188859912575
16	25.63876488008691	24.663547770982174	24.161515680942088	25.53617166798882
17	24.02891220952279	26.00633239735444	24.746366781201946	25.218388611920815
18	24.586544702491317	23.37403717084678	26.73914105519794	25.300277071463967
19	24.9576017847962	25.084854589824896	26.336212612218596	23.62133101316031
20	25.30144025980975	23.638546200677908	26.787529690382527	24.27248384912982
21	26.793810907449757	23.582713160080306	25.95026671908769	23.673209213382247
22	26.246414471924123	23.545258495346083	25.437300658597238	24.77102637413255
23	24.80731785052099	23.962377836143983	25.79742377045176	25.43288054288326
24	24.498607663550096	24.624464642563854	26.114741551181453	24.762186142704596
25	24.601898788655657	23.90817325923048	25.93561054593082	25.554317406183042
26	25.093229545914536	24.592360644220236	25.898621156534908	24.415788653330324
27	25.128823109295507	23.80488213412492	25.62922673565149	25.437068020928084
28	24.041009368318935	24.39671236445948	26.691915608359135	24.87036265886245
29	23.694379241275506	24.603527252339756	27.12299320930644	24.579100297078305
30	23.52525165579861	25.18419087455479	27.288631229745985	24.001926239900616
31	24.4202087690443	25.017389665769464	25.852791535711045	24.709610029475193
32	23.554796639781507	24.821276110670393	26.71448146226733	24.909445787280767
33	23.455692992720767	24.28365045724934	27.08088579118908	25.179770758840814
34	24.46417728851491	24.17966141913631	26.354590988081974	25.001570304266806
35	24.042405194333877	24.389965872053935	26.746120185272638	24.82150874833955
36	22.966688612153458	24.6800650454923	26.997834143300153	25.355412199054093
37	23.572477102637414	23.651108634812363	27.46962333634987	25.30679092620035
38	22.901550064789593	23.070910287935643	28.491367979285943	25.53617166798882
39	23.116041995752038	22.82291853261464	28.675849650927177	25.38518982070615
40	22.512812519628802	23.052531912072265	28.901973465347456	25.532682102951476
41	21.404294026097293	23.286100131905556	29.967221352415823	25.34238448958132
42	21.815830062835435	23.303780594761463	29.514973723575267	25.36541561882783
43	20.79571388358346	23.070910287935643	29.956054744296306	26.177321084184594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	94.5
2	104.0
3	278.0
4	452.0
5	452.0
6	567.5
7	683.0
8	716.0
9	749.0
10	1230.0
11	1711.0
12	1711.0
13	2788.0
14	3865.0
15	5478.5
16	7092.0
17	6323.0
18	5554.0
19	5554.0
20	6134.0
21	6714.0
22	5200.5
23	3687.0
24	3690.0
25	3693.0
26	3693.0
27	4040.0
28	4387.0
29	5068.0
30	5749.0
31	6656.5
32	7564.0
33	7564.0
34	8882.0
35	10200.0
36	11784.5
37	13369.0
38	15042.5
39	16716.0
40	16716.0
41	18165.0
42	19614.0
43	21249.5
44	22885.0
45	24974.5
46	27064.0
47	27064.0
48	29914.0
49	32764.0
50	32732.0
51	32700.0
52	31787.0
53	30874.0
54	30874.0
55	29859.5
56	28845.0
57	27881.0
58	26917.0
59	26304.0
60	25691.0
61	25691.0
62	24030.0
63	22369.0
64	20626.0
65	18883.0
66	17130.5
67	15378.0
68	15378.0
69	13411.0
70	11444.0
71	9829.0
72	8214.0
73	6581.0
74	4948.0
75	4948.0
76	3996.5
77	3045.0
78	2561.0
79	2077.0
80	1788.5
81	1500.0
82	1500.0
83	1208.5
84	917.0
85	790.5
86	664.0
87	559.0
88	454.0
89	454.0
90	324.0
91	194.0
92	110.0
93	26.0
94	17.0
95	8.0
96	8.0
97	5.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	429853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.84647274668725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.250036615873	62.13396686262227
2	5.500448575627813	7.5737296591303
3	1.5473409888590872	3.195869076579578
4	0.7036560700530184	1.9377695379978477
5	0.4425867679194517	1.5235268927805463
6	0.28191958984894055	1.16455016155754
7	0.1995234735563052	0.9615541169162979
8	0.13585380177449605	0.7482444049121356
9	0.11805189614102705	0.7314710985331178
>10	0.6985405539336459	9.246884420841033
>50	0.07606475056937363	3.5137695664015975
>100	0.04462465361316341	4.99775941094136
>500	0.0	0.0
>1k	0.0013522622307019217	2.2709047907863296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3244	0.7546765987442219	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2893	0.6730207768702324	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2419	0.5627505216899731	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.2793978406571549	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	482	0.11213135653351262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.326376691566652E-4	0.0
10	0.0	0.0	0.0	2.326376691566652E-4	0.0
11	0.0	0.0	0.0	0.0016284636840966562	0.0
12	0.0	0.0	0.0	0.002326376691566652	0.0
13	0.0	0.0	0.0	0.002559014360723317	0.0
14	0.0	0.0	0.0	0.003954840375663308	0.0
15	0.0	0.0	0.0	0.006048579398073295	0.0
16	0.0	0.0	0.0	0.011864521126989925	0.0
17	0.0	0.0	0.0	0.017680462855906555	0.0
18	0.0	0.0	0.0	0.020006839547473208	0.0
19	0.0	0.0	0.0	0.023031129246509855	0.0
20	0.0	0.0	0.0	0.024892230599763174	2.326376691566652E-4
21	0.0	0.0	0.0	0.029777621652053145	2.326376691566652E-4
22	0.0	0.0	0.0	0.04350324413229639	2.326376691566652E-4
23	0.0	0.0	0.0	0.05815941728916629	2.326376691566652E-4
24	0.0	0.0	0.0	0.08188845954314615	2.326376691566652E-4
25	0.0	0.0	0.0	0.09212451698603942	2.326376691566652E-4
26	0.0	0.0	0.0	0.10887442916531931	2.326376691566652E-4
27	0.0	0.0	0.0	0.1644748320937623	2.326376691566652E-4
28	0.0	0.0	0.0	0.32383163546607796	2.326376691566652E-4
29	0.0	0.0	0.0	0.5320423493612932	2.326376691566652E-4
30	0.0	0.0	0.0	0.8128360160333882	2.326376691566652E-4
31	0.0	0.0	0.0	1.4214161585472243	2.326376691566652E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	20	0.0018406544	37.0	11
ATAACGA	30	3.5957634E-4	30.833332	12
TATGCCG	25	0.0054925643	29.599998	13
AGCGTCA	70	6.5683707E-9	26.428572	3
GCAGCGT	85	6.91216E-11	26.117647	1
TAACGAA	40	0.0019292312	23.125002	13
TTGCGTT	40	0.0019292312	23.125002	4
CAGCGTC	105	9.767973E-10	21.142857	2
GATAACG	45	0.0038219702	20.555557	11
CTATAGT	45	0.0038219702	20.555557	4
TTTGACG	45	0.0038219702	20.555557	31
ACGAACG	45	0.0038219702	20.555557	15
GCGTCAG	95	1.6700324E-7	19.473684	4
TTAATTC	90	2.1458436E-6	18.5	3
CGAAAGC	80	1.6128366E-5	18.5	19
CGATAAC	60	9.223998E-4	18.5	10
CGAACGA	50	0.007027895	18.499998	16
TATGGGT	50	0.007027895	18.499998	3
AACGAAC	50	0.007027895	18.499998	14
ATACCGC	100	2.8645263E-7	18.499998	27
>>END_MODULE