FastQCFastQC Report
Fri 10 Feb 2017
ERR1631405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110833
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1670.15067714489366885No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1390.12541391101928126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10466196890817717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCT250.005475198429.619
GGCTGTT250.005475198429.620
CGAAAGC405.8925572E-527.7519
GCATTTG405.8925572E-527.7524
GAGCGAA405.8925572E-527.7516
CAAGACG405.8925572E-527.754
CAGAGCG405.8925572E-527.7514
ACTCTTT358.8187E-426.42857232
ACTTTCG358.8187E-426.42857215
GCGAAAG451.3127002E-424.66666618
GCGCAAG451.3127002E-424.6666661
AAGCATT451.3127002E-424.66666622
CGCAAGA451.3127002E-424.6666662
AGAGCGA451.3127002E-424.66666615
AGAGCAG451.3127002E-424.6666665
TCTATGG451.3127002E-424.6666662
ATACACA1002.0008883E-1124.05000137
CGTCTTC400.001920254323.12521
CGGTGAT400.001920254323.12514
AAGACGG502.6808094E-422.25
Produced by FastQC (version 0.11.5)