FastQCFastQC Report
Fri 10 Feb 2017
ERR1631404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498033
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11760.2361289312154014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11200.22488469639562036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.2188609991707377No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10240.20560886527599578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8430.16926589201920356No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8150.16364377460931304No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8040.16143508562685605No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7840.15741928747693426No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT7210.14476952330468063No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7040.1413560948772471No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6800.13653713709734094No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG6400.12850554079749735No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC6310.12669843163003255No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG6020.12087552431264595No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA5900.11846604542269287No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC5890.11826525551519677No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC5820.11685972616272415No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT5710.11465103718026716No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC5670.1138478775502828No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC5430.10902891977037667No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG5410.10862733995538448No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG5380.1080249702328962No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC5300.1064186509729275No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG5280.10601707115793532No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT5110.10260364273050179No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC5110.10260364273050179No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG5050.10139890328552527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT200.00184093537.09
TCGTATG200.00184093537.010
ATCTCGT303.5965748E-430.8333347
CGTATGC250.0054933929.611
TTAGAAC502.715551E-729.63
TATGCCG250.0054933929.613
TCTCGTA250.0054933929.68
GTACTAT652.9813236E-928.4615381
GTTCTAG905.456968E-1226.7222231
TCTAGAC901.4188117E-1024.6666683
ACTGATC1150.024.1304368
CTTAGAA551.8998531E-523.5454542
TAATGCC502.6991236E-422.24
ACGGAGT1107.4578566E-1121.86363626
ATACTGG705.092348E-621.1428576
CTATATC450.003822808920.5555574
TCTAATG450.003822808920.5555572
CATACAC450.003822808920.5555573
TAGATTG450.003822808920.5555575
CTCTAGT1001.2852979E-820.3527
Produced by FastQC (version 0.11.5)