FastQCFastQC Report
Fri 10 Feb 2017
ERR1631403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283968
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30791.0842771016452557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25910.9124267523101195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21040.740928555330178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.3211629479377958No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.13029637142213207No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3380.11902749605589363No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.1155059725039441No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT3050.10740646833446023No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT2990.10529355420329052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT250.005489462529.623
ATACTAC556.233877E-726.909093
TAGTCAG358.852887E-426.4285725
GTTTAAC400.001927627823.12533
AAACAGG400.001927627823.12523
TTAACGG400.001927627823.12535
CTCTATG3200.022.5468751
TACTGGT502.695165E-422.27
CTACTAA603.713581E-521.5833346
AGACCGC603.713581E-521.58333414
TCTATGG3450.021.4492742
AACAGAC705.07963E-621.14285911
TATACAC1151.3278623E-1020.91304437
CATGCAC450.003818823720.5555553
GACACAT450.003818823720.55555526
ACCACTC450.003818823720.5555558
TGTTTAC555.1299966E-420.18181814
ATCTTAC555.1299966E-420.1818181
ATGTTTA555.1299966E-420.18181831
CACATGT500.00702216218.528