##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631403.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283968
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.987773270227635	34.0	33.0	34.0	31.0	34.0
2	33.108378408834795	34.0	33.0	34.0	31.0	34.0
3	33.172142635789946	34.0	33.0	34.0	31.0	34.0
4	36.432545216362406	37.0	37.0	37.0	35.0	37.0
5	36.43427076290286	37.0	37.0	37.0	35.0	37.0
6	36.50863477574938	37.0	37.0	37.0	35.0	37.0
7	36.51681879648411	37.0	37.0	37.0	35.0	37.0
8	36.53148594207798	37.0	37.0	37.0	35.0	37.0
9	38.3539588967771	39.0	39.0	39.0	37.0	39.0
10	38.314929851250845	39.0	39.0	39.0	37.0	39.0
11	38.37288004282173	39.0	39.0	39.0	37.0	39.0
12	38.34093278115844	39.0	39.0	39.0	37.0	39.0
13	38.376415652467884	39.0	39.0	39.0	37.0	39.0
14	39.84613759296822	41.0	40.0	41.0	38.0	41.0
15	39.82986815415821	41.0	40.0	41.0	38.0	41.0
16	39.78005268199234	41.0	40.0	41.0	38.0	41.0
17	39.81221827811584	41.0	40.0	41.0	38.0	41.0
18	39.81974729546991	41.0	40.0	41.0	38.0	41.0
19	39.850071134775746	41.0	40.0	41.0	38.0	41.0
20	39.80798188528285	41.0	40.0	41.0	38.0	41.0
21	39.77901735406807	41.0	40.0	41.0	38.0	41.0
22	39.7104462474645	41.0	40.0	41.0	37.0	41.0
23	39.64574529524454	41.0	40.0	41.0	37.0	41.0
24	39.60854392044174	41.0	40.0	41.0	37.0	41.0
25	39.58538638156412	41.0	40.0	41.0	37.0	41.0
26	39.448191345503716	41.0	39.0	41.0	36.0	41.0
27	39.35605420329051	41.0	39.0	41.0	35.0	41.0
28	39.27553456727519	41.0	39.0	41.0	35.0	41.0
29	39.18386578769439	41.0	39.0	41.0	35.0	41.0
30	39.03556738787469	41.0	39.0	41.0	35.0	41.0
31	38.88615970813613	40.0	38.0	41.0	35.0	41.0
32	38.756296484110884	40.0	38.0	41.0	35.0	41.0
33	38.63296568627451	40.0	38.0	41.0	35.0	41.0
34	38.52401326910074	40.0	38.0	41.0	35.0	41.0
35	38.367301949515436	40.0	38.0	41.0	35.0	41.0
36	38.22460981519044	40.0	37.0	41.0	35.0	41.0
37	38.08609420779806	40.0	37.0	41.0	35.0	41.0
38	37.93449261888664	40.0	37.0	41.0	34.0	41.0
39	37.75309541920216	40.0	36.0	41.0	34.0	41.0
40	37.55930597813838	40.0	36.0	41.0	33.0	41.0
41	37.36144565584855	40.0	35.0	41.0	33.0	41.0
42	37.112819050033806	40.0	35.0	41.0	33.0	41.0
43	36.28678935654722	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	0.0
15	2.0
16	1.0
17	6.0
18	7.0
19	6.0
20	23.0
21	48.0
22	73.0
23	146.0
24	217.0
25	370.0
26	551.0
27	859.0
28	1208.0
29	1742.0
30	2268.0
31	2808.0
32	3498.0
33	4446.0
34	6153.0
35	8850.0
36	13631.0
37	31483.0
38	56054.0
39	149515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.929245548794235	18.845785440613028	12.78031327473518	24.444655735857562
2	20.292075163398692	20.650566260987155	32.80721771467208	26.25014086094208
3	20.45547385620915	23.321994027496057	29.456840207347305	26.76569190894749
4	14.307950191570882	16.664553752535497	33.54004676583277	35.48744929006085
5	14.983730561189992	36.90486251972053	31.697233491097588	16.414173427991887
6	31.745126211404102	34.40246788370521	16.785342010367366	17.067063894523326
7	29.097292652693262	31.00666272256029	20.366027157989635	19.530017466756817
8	29.923089925625423	30.146002366463826	18.928541244083842	21.00236646382691
9	28.234519382465628	13.253958192472393	20.7100800090151	37.801442416046875
10	20.718179513184584	25.784243295019156	28.660975884606714	24.836601307189543
11	35.55048456164075	22.712418300653596	19.47754676583277	22.259550371872887
12	23.13676188866351	28.33558710840658	25.20636128014424	23.321289722785664
13	31.68631676808655	19.112364773495607	24.395706558485465	24.805611899932387
14	23.289948163173317	21.610181428893398	25.327149537976112	29.77272086995718
15	28.16549752084742	26.380437232364212	21.99578825783187	23.458276988956502
16	24.960206783862972	25.062683119224697	24.503817331530314	25.473292765382016
17	23.713235294117645	27.09072853279243	24.661229434302456	24.534806738787466
18	23.312133761550598	23.78190500338066	27.349560513860716	25.55640072120802
19	24.20871365787694	25.697965967996396	27.677414356547214	22.415906017579445
20	24.53621534820825	23.822754676583276	28.557090939824203	23.08393903538427
21	26.105406242956953	24.438316993464053	26.93578149650665	22.520495267072345
22	26.024411201262115	24.452050935316656	26.0532876943881	23.47025016903313
23	24.230194951543833	24.424935203966644	27.01712869055668	24.327741153932838
24	23.340658102321388	26.045188190218617	26.26880493576741	24.345348771692585
25	23.607941739914356	24.83589700247915	26.937190105927428	24.618971151679062
26	23.89670667117422	25.63563500112689	26.758296709488395	23.709361618210504
27	24.015382014874913	25.216573698444893	26.475870520622042	24.29217376605815
28	23.021960220869957	25.432090939824203	27.737632409285553	23.808316430020284
29	23.34488393058373	25.484561640748254	27.990829952670726	23.179724475997297
30	22.40393283750282	25.918061189993242	28.487364773495607	23.19064119900834
31	23.787891593418976	25.975462023890017	26.75512733829164	23.48151904439937
32	21.853870858688303	25.52822853279243	27.84750394410638	24.770396664412893
33	21.541159567275187	25.51872041920216	28.53173597025017	24.408384043272484
34	23.550188753662386	24.88273326572008	27.818979603335585	23.74809837728195
35	23.125845165652468	25.875098602659452	27.803132747351817	23.195923484336266
36	22.58986928104575	25.419413455037187	27.49218221771467	24.498535046202388
37	22.174681654270902	24.40134099616858	28.44123281496507	24.982744534595447
38	21.93134437683119	23.671681316204644	29.326543835925172	25.07043047103899
39	21.792948501239575	23.701966418751407	29.832234617985126	24.67285046202389
40	21.272819472616632	24.117154045526256	30.2523523777327	24.357674104124406
41	20.43857054315979	23.700909961685824	31.149636578769442	24.710882916384943
42	20.192416046878524	24.505930245661485	30.95137480279468	24.350278904665316
43	19.038412778904664	23.322698332206446	31.445796709488395	26.193092179400495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	43.0
2	51.0
3	187.5
4	324.0
5	324.0
6	426.5
7	529.0
8	583.0
9	637.0
10	1022.0
11	1407.0
12	1407.0
13	2419.0
14	3431.0
15	5008.0
16	6585.0
17	5658.5
18	4732.0
19	4732.0
20	5090.0
21	5448.0
22	3886.0
23	2324.0
24	2170.0
25	2016.0
26	2016.0
27	2150.5
28	2285.0
29	2883.5
30	3482.0
31	4628.0
32	5774.0
33	5774.0
34	7056.0
35	8338.0
36	9533.0
37	10728.0
38	11674.0
39	12620.0
40	12620.0
41	14256.0
42	15892.0
43	16726.5
44	17561.0
45	18344.0
46	19127.0
47	19127.0
48	20244.5
49	21362.0
50	21135.5
51	20909.0
52	20149.0
53	19389.0
54	19389.0
55	18062.0
56	16735.0
57	15866.5
58	14998.0
59	14534.5
60	14071.0
61	14071.0
62	13304.5
63	12538.0
64	11843.5
65	11149.0
66	10321.5
67	9494.0
68	9494.0
69	8149.5
70	6805.0
71	6015.0
72	5225.0
73	4170.0
74	3115.0
75	3115.0
76	2489.5
77	1864.0
78	1498.0
79	1132.0
80	909.0
81	686.0
82	686.0
83	557.5
84	429.0
85	407.5
86	386.0
87	304.5
88	223.0
89	223.0
90	158.0
91	93.0
92	60.0
93	27.0
94	17.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	283968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.711402773067476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04647309220795	38.32505850719769
2	7.666786874901776	7.1625273937760845
3	2.9021351238526445	4.066884080164409
4	1.5939273561733276	2.978183309008916
5	1.040859905671605	2.431001314208168
6	0.7510053809789777	2.1048308901390014
7	0.5982846856934361	1.9562701848468926
8	0.40660213945022144	1.5194365043400195
9	0.36283597623900743	1.5253719683993479
>10	2.318798651801821	20.70057561119929
>50	0.2119655035295081	6.889266188436427
>100	0.09730800695838628	7.280029087524806
>500	7.543256353363277E-4	0.3213487501971165
>1k	0.002262976906008983	2.7392162105618243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3079	1.0842771016452557	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2591	0.9124267523101195	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2104	0.740928555330178	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.3211629479377958	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	370	0.13029637142213207	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	338	0.11902749605589363	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	328	0.1155059725039441	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	305	0.10740646833446023	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	299	0.10529355420329052	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.521523551949515E-4	0.0
10	0.0	0.0	0.0	3.521523551949515E-4	0.0
11	0.0	0.0	0.0	0.0010564570655848546	0.0
12	0.0	0.0	0.0	0.0010564570655848546	0.0
13	0.0	0.0	0.0	0.0010564570655848546	0.0
14	0.0	0.0	0.0	0.001408609420779806	0.0
15	0.0	0.0	0.0	0.002465066486364661	0.0
16	0.0	0.0	0.0	0.003873675907144467	0.0
17	0.0	3.521523551949515E-4	0.0	0.006338742393509128	0.0
18	0.0	3.521523551949515E-4	0.0	0.006690894748704079	0.0
19	0.0	3.521523551949515E-4	0.0	0.007043047103899031	0.0
20	0.0	3.521523551949515E-4	0.0	0.008803808879873788	0.0
21	0.0	3.521523551949515E-4	0.0	0.010212418300653595	0.0
22	0.0	3.521523551949515E-4	0.0	0.012677484787018255	0.0
23	0.0	3.521523551949515E-4	0.0	0.019016227180527385	0.0
24	0.0	3.521523551949515E-4	0.0	0.02993295019157088	0.0
25	0.0	3.521523551949515E-4	0.0	0.03662384494027496	0.0
26	0.0	3.521523551949515E-4	0.0	0.051414243858462924	0.0
27	0.0	3.521523551949515E-4	0.0	0.10811077304485013	0.0
28	0.0	3.521523551949515E-4	0.0	0.24932386747802568	0.0
29	0.0	3.521523551949515E-4	0.0	0.4472334910975885	0.0
30	0.0	3.521523551949515E-4	0.0	0.7074740815866577	0.0
31	0.0	3.521523551949515E-4	0.0	1.329375140860942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATT	25	0.0054894625	29.6	23
ATACTAC	55	6.233877E-7	26.90909	3
TAGTCAG	35	8.852887E-4	26.428572	5
GTTTAAC	40	0.0019276278	23.125	33
AAACAGG	40	0.0019276278	23.125	23
TTAACGG	40	0.0019276278	23.125	35
CTCTATG	320	0.0	22.546875	1
TACTGGT	50	2.695165E-4	22.2	7
CTACTAA	60	3.713581E-5	21.583334	6
AGACCGC	60	3.713581E-5	21.583334	14
TCTATGG	345	0.0	21.449274	2
AACAGAC	70	5.07963E-6	21.142859	11
TATACAC	115	1.3278623E-10	20.913044	37
CATGCAC	45	0.0038188237	20.555555	3
GACACAT	45	0.0038188237	20.555555	26
ACCACTC	45	0.0038188237	20.555555	8
TGTTTAC	55	5.1299966E-4	20.181818	14
ATCTTAC	55	5.1299966E-4	20.181818	1
ATGTTTA	55	5.1299966E-4	20.181818	31
CACATGT	50	0.007022162	18.5	28
>>END_MODULE