FastQCFastQC Report
Fri 10 Feb 2017
ERR1631402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731804
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16930.23134609813556636No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15790.21576815650092102No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14070.1922645954381228No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11710.16001552328219032No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT10910.14908363441577252No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10900.1489469858049423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.14689725664248895No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC10170.13897163721433609No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9660.13200255806199473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8970.1225738039147094No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG8950.12230050669304897No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC8850.12093402058474673No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8790.1201141289197654No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG8410.11492148170821695No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8400.11478483309738674No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC8240.11259845532410317No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC7990.10918224005334763No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT7980.1090455914425174No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG7870.10754245672338494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7730.10562937617176185No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC7570.10344299839847829No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7560.10330634978764806No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA7370.10071002618187383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC358.8663044E-426.428578
ACACCGT851.9736035E-923.9411756
CCGCTTA952.8012437E-1023.36842225
AGTCGGT3350.021.53731511
CAAGACG2750.021.5272734
GCAGTCG3500.020.6142869
TAGCACC450.003824500120.5555554
CGCCCGA450.003824500120.55555510
AAGACGG2900.020.4137945
CTTGCCG555.140639E-420.1818189
TCTAGAC1202.382876E-1020.0416683
CTAGACA1303.274181E-1119.9230774
TGTCGTA759.258229E-619.73333415
ACATGTA851.2440432E-619.5882348
AGACGGA3050.019.4098366
ACGGACC3150.019.380958
CGCTTAT1153.0449883E-919.30434826
TCGGTGA3950.019.20253213
GGTATCA8000.019.193751
CGGTGAT3900.018.9743614
Produced by FastQC (version 0.11.5)