FastQCFastQC Report
Fri 10 Feb 2017
ERR1631401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526384
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31820.20846654577092003No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC27040.17715070388578497No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC26730.1751197601652009No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT23520.15408966551012065No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22860.1497657208146836No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22510.14747271983983062No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC22140.14504869023784317No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC19830.12991488380381344No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG19270.12624608224404868No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC19100.1251323389134058No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC17840.11687753540393506No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC17110.1120949905135274No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA16530.10829516032662816No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16240.10639524523317855No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG16240.10639524523317855No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG15900.1041677585718928No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG15870.1039712156311911No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA15590.10213681485130871No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG15410.1009575572070986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.023.61
CTAGACA3650.023.315074
TCTAGAC3450.023.057973
TTAGGAC1054.0017767E-1122.904763
GCTATAC450.003826375620.5555533
GCCTAAT1800.020.55555316
GTTCTAG3900.019.9230751
TATACAC4400.018.92045437
TAGAACT3750.018.7466664
CGAATCA1700.018.526
ACCGTTA500.007035912518.58
CCGTTAT500.007035912518.59
ATACAGT701.21952966E-418.56
CGAACGA4050.018.27160516
ACGAACG4050.018.27160515
ACATGTA2850.018.1754388
ATACACA2750.018.16363537
TGCCTAA1950.018.0256415
GTCTAGA3200.017.9218751
GTATCAA11500.017.8565222
Produced by FastQC (version 0.11.5)