##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631401.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1526384
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.076045084330026	34.0	33.0	34.0	31.0	34.0
2	33.20941257245883	34.0	33.0	34.0	31.0	34.0
3	33.30192205893144	34.0	34.0	34.0	31.0	34.0
4	36.527799688675984	37.0	37.0	37.0	35.0	37.0
5	36.518654545645134	37.0	37.0	37.0	35.0	37.0
6	36.56730875061584	37.0	37.0	37.0	35.0	37.0
7	36.56937769263829	37.0	37.0	37.0	35.0	37.0
8	36.56474517552595	37.0	37.0	37.0	35.0	37.0
9	38.418159519491816	39.0	39.0	39.0	37.0	39.0
10	38.372015167873876	39.0	39.0	39.0	37.0	39.0
11	38.434416896403526	39.0	39.0	39.0	37.0	39.0
12	38.38914847115798	39.0	39.0	39.0	37.0	39.0
13	38.414542474239774	39.0	39.0	39.0	37.0	39.0
14	39.96707119571484	41.0	40.0	41.0	38.0	41.0
15	39.96800674011258	41.0	40.0	41.0	38.0	41.0
16	39.94935547018313	41.0	40.0	41.0	38.0	41.0
17	39.9345597176071	41.0	40.0	41.0	38.0	41.0
18	39.908854521535865	41.0	40.0	41.0	38.0	41.0
19	39.91559463411566	41.0	40.0	41.0	38.0	41.0
20	39.88833543852661	41.0	40.0	41.0	38.0	41.0
21	39.851140997285086	41.0	40.0	41.0	38.0	41.0
22	39.80370339311733	41.0	40.0	41.0	38.0	41.0
23	39.743408604912	41.0	40.0	41.0	38.0	41.0
24	39.71501208083942	41.0	40.0	41.0	37.0	41.0
25	39.7013182790176	41.0	40.0	41.0	37.0	41.0
26	39.633635441671295	41.0	40.0	41.0	37.0	41.0
27	39.57515081394983	41.0	40.0	41.0	37.0	41.0
28	39.48760534701621	41.0	40.0	41.0	37.0	41.0
29	39.44103711778949	41.0	39.0	41.0	37.0	41.0
30	39.381146552898876	41.0	39.0	41.0	36.0	41.0
31	39.35049502615331	41.0	39.0	41.0	36.0	41.0
32	39.26926448390444	41.0	39.0	41.0	35.0	41.0
33	39.188719221375486	41.0	39.0	41.0	35.0	41.0
34	39.14337152381052	41.0	39.0	41.0	35.0	41.0
35	39.045244840092664	41.0	39.0	41.0	35.0	41.0
36	38.98477971467206	41.0	39.0	41.0	35.0	41.0
37	38.92797945995241	40.0	39.0	41.0	35.0	41.0
38	38.848721553685046	40.0	38.0	41.0	35.0	41.0
39	38.7757687449554	40.0	38.0	41.0	35.0	41.0
40	38.67804497426598	40.0	38.0	41.0	35.0	41.0
41	38.59584613046258	40.0	38.0	41.0	35.0	41.0
42	38.45755131081039	40.0	37.0	41.0	35.0	41.0
43	37.62361306198178	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	1.0
15	2.0
16	4.0
17	13.0
18	18.0
19	43.0
20	74.0
21	149.0
22	287.0
23	461.0
24	767.0
25	1147.0
26	1815.0
27	2511.0
28	3604.0
29	4730.0
30	6665.0
31	8687.0
32	11670.0
33	16233.0
34	25082.0
35	38255.0
36	65236.0
37	125519.0
38	303518.0
39	909889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.692095829096736	16.782408620635437	10.767998092223188	27.757497458044632
2	20.35962117003323	19.520186270296332	31.253734315873334	28.866458243797105
3	20.542406104885796	20.025039570645394	28.408513191962182	31.024041132506632
4	14.50834128240338	14.114403714923638	32.79201039843185	38.58524460424114
5	17.1751014161574	33.98587773456745	31.69078030168031	17.14824054759484
6	37.47497353221732	33.047057621149065	14.638059623266491	14.83990922336712
7	32.37678067904275	28.33304070273273	19.254132642899823	20.03604597532469
8	29.941613643748887	29.49015450895712	19.03839400832294	21.52983783897106
9	27.80558496420298	12.79212832419627	17.834961582406525	41.567325129194224
10	18.630698434994077	24.098981645509912	29.891036593674986	27.379283325821024
11	40.35439312781056	19.409794651935556	19.239260893720058	20.996551326533822
12	24.94064403190809	23.347335925953104	25.756231721506516	25.95578832063229
13	32.00780406503213	16.3540760385329	23.69148261512175	27.94663728131322
14	24.6187722093523	18.62689860480718	21.47971938909213	35.27460979674839
15	27.846727953123196	25.246661390580616	19.735531818991813	27.171078837304375
16	28.321117097663496	23.188856801433978	21.217793163450352	27.272232937452173
17	27.194991561756414	23.455369029025462	21.960528936362017	27.38911047285611
18	27.84830029664881	21.11644252036185	23.555933500351156	27.479323682638185
19	27.414792083774465	22.421684189561734	22.276569985010326	27.886953741653475
20	27.472510194027187	21.731163324563152	22.53423778027023	28.262088701139426
21	29.617645363158946	22.409367498611097	22.337563810941415	25.63542332728855
22	29.083834736213166	21.62955062422038	21.707643685992515	27.57897095357394
23	27.175795867881213	21.522041635656556	22.90236270820449	28.39979978825774
24	26.793454333902872	22.782340485749327	22.312013228650194	28.112191951697607
25	27.50251574964098	21.732408096520928	22.599359007956057	28.16571714588203
26	27.951485340517195	22.793674461996456	22.10767408463401	27.147166112852332
27	28.112716066206143	21.63046782461032	22.44238671264898	27.814429396534557
28	26.680638685940107	21.941922871308925	23.568905334437467	27.808533108313505
29	26.20087736768729	22.343984213671003	23.398109518967704	28.057028899674002
30	25.44942819107118	23.462641117831424	24.19043962724976	26.897491063847628
31	27.872802649922956	22.809659954506863	21.75101416157402	27.566523233996165
32	26.623379175882345	22.392137234142915	22.22487919160578	28.759604398368953
33	26.139752513129068	21.685368819379658	23.48937095776685	28.685507709724423
34	27.686545456451327	21.689496221134394	23.27441849495278	27.349539827461506
35	27.336699022002325	21.875032757156784	24.184281281774442	26.60398693906645
36	25.918117590331136	22.72789809117496	23.88068795270391	27.473296365789995
37	27.001462279478822	21.5929281229363	24.14254866403212	27.26306093355276
38	25.734415455088627	20.94531913332425	25.47628905963375	27.843976351953376
39	26.675659598108997	20.710450319185735	24.990238367278483	27.62365171542679
40	25.76029360894768	21.769423683686412	25.41542626232979	27.054856445036112
41	25.22825186846822	21.20645918720322	26.743270369710377	26.822018574618184
42	26.159406807199236	21.139700101678216	26.766003836518205	25.93488925460435
43	23.87308829233011	20.154757911508504	26.965953521525382	29.006200274636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	25.0
2	31.0
3	87.0
4	143.0
5	143.0
6	186.5
7	230.0
8	195.0
9	160.0
10	260.5
11	361.0
12	361.0
13	585.5
14	810.0
15	1335.5
16	1861.0
17	1807.0
18	1753.0
19	1753.0
20	2007.5
21	2262.0
22	2132.5
23	2003.0
24	2491.5
25	2980.0
26	2980.0
27	3921.5
28	4863.0
29	6342.5
30	7822.0
31	10291.5
32	12761.0
33	12761.0
34	17778.0
35	22795.0
36	30397.0
37	37999.0
38	46154.5
39	54310.0
40	54310.0
41	59293.0
42	64276.0
43	70322.5
44	76369.0
45	86808.0
46	97247.0
47	97247.0
48	104504.5
49	111762.0
50	116862.0
51	121962.0
52	127267.5
53	132573.0
54	132573.0
55	129378.5
56	126184.0
57	124162.0
58	122140.0
59	121481.5
60	120823.0
61	120823.0
62	113892.5
63	106962.0
64	99261.5
65	91561.0
66	82476.0
67	73391.0
68	73391.0
69	59954.0
70	46517.0
71	40466.5
72	34416.0
73	25477.0
74	16538.0
75	16538.0
76	12966.0
77	9394.0
78	7928.0
79	6462.0
80	5851.0
81	5240.0
82	5240.0
83	4272.0
84	3304.0
85	3065.5
86	2827.0
87	2449.0
88	2071.0
89	2071.0
90	1537.0
91	1003.0
92	566.0
93	129.0
94	88.0
95	47.0
96	47.0
97	32.0
98	17.0
99	11.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1526384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.80075671804274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.10718231428909	32.63422319216798
2	7.689440380350371	5.967122109917376
3	2.535864453748288	2.9518037921945903
4	1.2690100464147294	1.9695420033476014
5	0.8086083374036936	1.5687307689890868
6	0.5358091819611803	1.2473881029941554
7	0.38306257202737576	1.040418236551535
8	0.292356438540842	0.9074920837421301
9	0.23452013621599027	0.8189602875718996
>10	1.6296924604924126	12.697977178063272
>50	0.2475890931825551	6.721020146357424
>100	0.2300498304757701	18.28423500775903
>500	0.025617640504871506	6.870995556138306
>1k	0.011197114392857744	6.320091534205738
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3182	0.20846654577092003	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2704	0.17715070388578497	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2673	0.1751197601652009	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2352	0.15408966551012065	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2286	0.1497657208146836	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2251	0.14747271983983062	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2214	0.14504869023784317	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1983	0.12991488380381344	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1927	0.12624608224404868	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1910	0.1251323389134058	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1784	0.11687753540393506	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1711	0.1120949905135274	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1653	0.10829516032662816	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1624	0.10639524523317855	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	1624	0.10639524523317855	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	1590	0.1041677585718928	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1587	0.1039712156311911	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	1559	0.10213681485130871	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1541	0.1009575572070986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.551431356722817E-5	0.0	0.0	0.0
6	0.0	6.551431356722817E-5	0.0	0.0	0.0
7	0.0	6.551431356722817E-5	0.0	0.0	0.0
8	0.0	6.551431356722817E-5	0.0	0.0	0.0
9	0.0	6.551431356722817E-5	0.0	6.551431356722817E-5	0.0
10	0.0	6.551431356722817E-5	0.0	6.551431356722817E-5	0.0
11	0.0	6.551431356722817E-5	0.0	6.551431356722817E-5	0.0
12	0.0	6.551431356722817E-5	0.0	6.551431356722817E-5	0.0
13	0.0	6.551431356722817E-5	0.0	6.551431356722817E-5	0.0
14	0.0	6.551431356722817E-5	0.0	6.551431356722817E-5	0.0
15	0.0	6.551431356722817E-5	0.0	2.620572542689127E-4	0.0
16	0.0	6.551431356722817E-5	0.0	2.620572542689127E-4	0.0
17	0.0	6.551431356722817E-5	0.0	4.5860019497059716E-4	6.551431356722817E-5
18	0.0	6.551431356722817E-5	0.0	5.896288221050535E-4	6.551431356722817E-5
19	0.0	6.551431356722817E-5	0.0	7.86171762806738E-4	6.551431356722817E-5
20	0.0	6.551431356722817E-5	0.0	8.516860763739662E-4	6.551431356722817E-5
21	0.0	6.551431356722817E-5	0.0	0.0013102862713445633	6.551431356722817E-5
22	0.0	6.551431356722817E-5	0.0	0.002227486661285758	6.551431356722817E-5
23	0.0	6.551431356722817E-5	0.0	0.002817115483390811	6.551431356722817E-5
24	0.0	6.551431356722817E-5	0.0	0.004454973322571516	6.551431356722817E-5
25	0.0	6.551431356722817E-5	0.0	0.005503202339647166	2.620572542689127E-4
26	0.0	6.551431356722817E-5	0.0	0.008647889390874118	2.620572542689127E-4
27	0.0	6.551431356722817E-5	0.0	0.02509198209624839	2.620572542689127E-4
28	0.0	6.551431356722817E-5	0.0	0.09551986918101867	2.620572542689127E-4
29	0.0	1.3102862713445634E-4	0.0	0.20171857147349553	2.620572542689127E-4
30	0.0	1.3102862713445634E-4	0.0	0.3442122034822168	2.620572542689127E-4
31	0.0	1.3102862713445634E-4	0.0	0.71639901885764	2.620572542689127E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	925	0.0	23.6	1
CTAGACA	365	0.0	23.31507	4
TCTAGAC	345	0.0	23.05797	3
TTAGGAC	105	4.0017767E-11	22.90476	3
GCTATAC	45	0.0038263756	20.555553	3
GCCTAAT	180	0.0	20.555553	16
GTTCTAG	390	0.0	19.923075	1
TATACAC	440	0.0	18.920454	37
TAGAACT	375	0.0	18.746666	4
CGAATCA	170	0.0	18.5	26
ACCGTTA	50	0.0070359125	18.5	8
CCGTTAT	50	0.0070359125	18.5	9
ATACAGT	70	1.21952966E-4	18.5	6
CGAACGA	405	0.0	18.271605	16
ACGAACG	405	0.0	18.271605	15
ACATGTA	285	0.0	18.175438	8
ATACACA	275	0.0	18.163635	37
TGCCTAA	195	0.0	18.02564	15
GTCTAGA	320	0.0	17.921875	1
GTATCAA	1150	0.0	17.856522	2
>>END_MODULE