FastQCFastQC Report
Fri 10 Feb 2017
ERR1631400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704738
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103161.4638064074876052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76681.0880639329793484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75101.0656442536091426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.4360485740800127No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA14730.20901384628046168No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11110.15764723911581324No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.1433156719234666No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA9480.134518076221234No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9300.13196393553348904No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8800.12486910028975307No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.12089599255326092No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8220.11663909140701935No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8200.11635529799726992No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8050.11422684742414912No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.11351736389977553No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA7880.11181460344127887No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.10471976819754292No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.10429407808291875No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT7320.10386838796829459No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC7150.10145614398542437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG250.005494918729.5999989
TCGTATG250.005494918729.59999810
AGGAGTA601.3361805E-624.6666685
GTATATC400.001930449423.1250021
CTAGGAC400.001930449423.1250023
TAGGAGT1005.329639E-1022.1999994
AGTCGGT9350.020.5775411
CGAGCCG1900.020.44736915
TTAAGAC1101.7516868E-920.18181819
ATTGACT1650.020.18181831
CAGTCGG9550.020.14659910
GCAGTCG9650.020.1295329
TCGGTGA9900.019.62121213
GGCAGTC10050.019.5124388
GGGCAGT10600.019.3726437
TATTGAC1551.8189894E-1219.09677530
CGGTGAT10100.019.04950514
GTCGGTG10100.019.04950512
AAGACGG2150.018.9302335
TGGGCAG10900.018.8394496
Produced by FastQC (version 0.11.5)