##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631400.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704738
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02244522077709	34.0	33.0	34.0	31.0	34.0
2	33.12609083091873	34.0	33.0	34.0	31.0	34.0
3	33.1841648953228	34.0	33.0	34.0	31.0	34.0
4	36.44947767822936	37.0	37.0	37.0	35.0	37.0
5	36.44542227040404	37.0	37.0	37.0	35.0	37.0
6	36.51476860904336	37.0	37.0	37.0	35.0	37.0
7	36.51783925373685	37.0	37.0	37.0	35.0	37.0
8	36.533403903294555	37.0	37.0	37.0	35.0	37.0
9	38.36438222431599	39.0	39.0	39.0	37.0	39.0
10	38.33144232324637	39.0	39.0	39.0	37.0	39.0
11	38.385915049280726	39.0	39.0	39.0	37.0	39.0
12	38.36051128220701	39.0	39.0	39.0	37.0	39.0
13	38.381892277697524	39.0	39.0	39.0	37.0	39.0
14	39.85133340333571	41.0	40.0	41.0	38.0	41.0
15	39.82431910866166	41.0	40.0	41.0	38.0	41.0
16	39.77296527220045	41.0	40.0	41.0	38.0	41.0
17	39.80644438074859	41.0	40.0	41.0	38.0	41.0
18	39.82691298042677	41.0	40.0	41.0	38.0	41.0
19	39.85732995808371	41.0	40.0	41.0	38.0	41.0
20	39.840099441210775	41.0	40.0	41.0	38.0	41.0
21	39.809929363820316	41.0	40.0	41.0	38.0	41.0
22	39.73584935110637	41.0	40.0	41.0	37.0	41.0
23	39.65338750003547	41.0	40.0	41.0	37.0	41.0
24	39.607903930254935	41.0	40.0	41.0	37.0	41.0
25	39.59795980917731	41.0	40.0	41.0	37.0	41.0
26	39.46824777435018	41.0	39.0	41.0	36.0	41.0
27	39.383728421058606	41.0	39.0	41.0	35.0	41.0
28	39.262630935184426	41.0	39.0	41.0	35.0	41.0
29	39.15694343145964	41.0	39.0	41.0	35.0	41.0
30	39.01577181874683	41.0	39.0	41.0	35.0	41.0
31	38.86945219358116	40.0	38.0	41.0	35.0	41.0
32	38.718502478935434	40.0	38.0	41.0	35.0	41.0
33	38.57527194503489	40.0	38.0	41.0	35.0	41.0
34	38.46585681487304	40.0	38.0	41.0	35.0	41.0
35	38.309303599351814	40.0	37.0	41.0	35.0	41.0
36	38.170501661610416	40.0	37.0	41.0	35.0	41.0
37	38.02896821230017	40.0	37.0	41.0	35.0	41.0
38	37.87677690148679	40.0	37.0	41.0	35.0	41.0
39	37.69414023367549	40.0	36.0	41.0	34.0	41.0
40	37.49296476137231	40.0	35.0	41.0	33.0	41.0
41	37.28222687012762	40.0	35.0	41.0	33.0	41.0
42	37.02724700527004	40.0	35.0	41.0	33.0	41.0
43	36.15862348844535	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	7.0
12	12.0
13	7.0
14	1.0
15	10.0
16	8.0
17	9.0
18	11.0
19	24.0
20	52.0
21	107.0
22	189.0
23	351.0
24	551.0
25	885.0
26	1402.0
27	2109.0
28	3155.0
29	4632.0
30	6031.0
31	7167.0
32	8619.0
33	10826.0
34	15719.0
35	21996.0
36	34397.0
37	81655.0
38	129126.0
39	375673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76216693295949	17.075281877804233	13.857632198065096	24.30491899117119
2	18.673890154922823	21.58546864224719	34.16532101291544	25.575320189914553
3	20.01864522702054	23.830132616660375	29.540056020819083	26.611166135500003
4	13.838901833021634	16.405103740680936	32.638654365168335	37.1173400611291
5	14.83487480453729	37.367645848528106	31.236714921006104	16.5607644259285
6	32.10044016357852	33.788443364768185	17.497708368216273	16.61340810343702
7	29.01220027868513	31.33192760997704	20.208219224733163	19.447652886604665
8	30.377104682875057	30.386044175282162	19.33257465895127	19.904276482891515
9	28.450715017495863	13.708498761241767	19.368758318694322	38.472027902568044
10	19.377555914396556	26.56930660756196	28.186787146428887	25.866350331612598
11	35.3610845448947	23.327960178108746	19.2919922013571	22.01896307563946
12	23.494546909631666	27.751306159168372	26.452809412859814	22.30133751834015
13	31.252181661837447	18.61131370807307	25.82065959264294	24.31584503744654
14	23.583799936997863	21.356873050694016	25.19886823188192	29.860458780426203
15	27.093614932074043	26.768813374615814	22.653808933248953	23.483762760061186
16	24.4131861769906	25.620443342064707	25.131892987181054	24.83447749376364
17	22.855586047580804	27.394152152998704	25.35850202486598	24.391759774554515
18	23.33391983971348	23.20394245804824	28.673492844149173	24.788644858089107
19	23.707817657058367	26.050248461130238	28.471857626522194	21.7700762552892
20	24.737136354219583	23.256444238851888	28.881655310200387	23.124764096728146
21	26.830680337941192	23.933007727694548	27.477161725350413	21.759150209013846
22	25.700614980318925	23.680573489722423	26.472674951542274	24.146136578416378
23	23.218274025240586	24.69016854490605	27.517460389534836	24.57409704031853
24	23.086451986411973	25.69309445496057	28.221268045713444	22.999185512914018
25	22.868498647724405	24.57948911510377	27.763225482377845	24.78878675479398
26	23.954576026835504	25.32373733217167	27.80508501031589	22.916601630676933
27	24.26348515334777	24.3098853758418	27.052039197545753	24.374590273264673
28	22.275938008167575	25.242288623573582	28.958847117652237	23.522926250606606
29	22.137021134095225	25.593766761548263	29.292871960927325	22.976340143429187
30	21.44300435055297	26.66764102404014	29.658823562799224	22.230531062607664
31	23.067579724663634	26.2081794936558	27.456444806438707	23.26779597524186
32	21.792779728069156	25.6889794505192	28.608646050021424	23.909594771390218
33	21.729493797695028	25.035687021275994	29.34579943184559	23.889019749183387
34	23.032247445149828	25.383333948219054	27.943008607454118	23.641409999177
35	22.894323848011602	25.594050554958013	28.273769826517086	23.237855770513296
36	20.698046649960695	26.43578180827485	28.74898189114252	24.117189650621935
37	22.188813431374495	25.072580164543417	28.82759266564312	23.911013738438967
38	21.292168153271145	23.82090933084352	30.385618485167537	24.5013040307178
39	21.908567439246927	23.567907506051895	30.188523962096554	24.335001092604628
40	21.02171303377993	24.205449401054008	30.271959224562888	24.500878340603176
41	19.489086724428084	24.99652353073057	31.62295775167509	23.891431993166254
42	20.732669445950126	24.49435109217894	30.883959712687552	23.889019749183387
43	18.933135434728936	24.778712088747877	31.16136777071763	25.126784705805562
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93.0
1	123.5
2	154.0
3	626.0
4	1098.0
5	1098.0
6	1568.5
7	2039.0
8	2027.5
9	2016.0
10	3073.0
11	4130.0
12	4130.0
13	6905.5
14	9681.0
15	14947.5
16	20214.0
17	17788.5
18	15363.0
19	15363.0
20	15996.5
21	16630.0
22	12090.5
23	7551.0
24	7461.5
25	7372.0
26	7372.0
27	7459.0
28	7546.0
29	9109.5
30	10673.0
31	11341.0
32	12009.0
33	12009.0
34	13846.5
35	15684.0
36	18236.5
37	20789.0
38	24612.0
39	28435.0
40	28435.0
41	30869.5
42	33304.0
43	34989.0
44	36674.0
45	41480.5
46	46287.0
47	46287.0
48	51771.5
49	57256.0
50	56683.0
51	56110.0
52	54267.5
53	52425.0
54	52425.0
55	47489.0
56	42553.0
57	40813.0
58	39073.0
59	37151.0
60	35229.0
61	35229.0
62	33000.0
63	30771.0
64	28568.5
65	26366.0
66	23772.5
67	21179.0
68	21179.0
69	18277.0
70	15375.0
71	13513.0
72	11651.0
73	8905.0
74	6159.0
75	6159.0
76	5048.5
77	3938.0
78	3341.0
79	2744.0
80	2456.5
81	2169.0
82	2169.0
83	1788.0
84	1407.0
85	1289.5
86	1172.0
87	1064.0
88	956.0
89	956.0
90	672.0
91	388.0
92	219.5
93	51.0
94	34.0
95	17.0
96	17.0
97	10.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	704738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.021069735184476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.26624125451988	41.50373085291396
2	5.68134907488178	5.34286222079884
3	1.7751911595080647	2.5041416191353507
4	0.8952802522788263	1.68388140699745
5	0.5391038671299266	1.2674620265411953
6	0.3738809881421714	1.0548170409655633
7	0.27775556796992074	0.914225475159459
8	0.213522074850677	0.8032029097243969
9	0.1770196165410088	0.7491286560483352
>10	1.4065658935927372	13.962515595979363
>50	0.20959673657957958	6.831129799146236
>100	0.1733029238116592	15.922758186530778
>500	0.009073451508463806	2.8863847892056143
>1k	0.0012097935344618407	0.9481438406402162
>5k	6.048967672309203E-4	2.158531155427809
>10k+	3.024483836154602E-4	1.4670844247854313
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10316	1.4638064074876052	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7668	1.0880639329793484	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7510	1.0656442536091426	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3073	0.4360485740800127	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1473	0.20901384628046168	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1111	0.15764723911581324	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1010	0.1433156719234666	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	948	0.134518076221234	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	930	0.13196393553348904	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	880	0.12486910028975307	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	852	0.12089599255326092	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	822	0.11663909140701935	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	820	0.11635529799726992	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	805	0.11422684742414912	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.11351736389977553	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	788	0.11181460344127887	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	738	0.10471976819754292	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	735	0.10429407808291875	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	732	0.10386838796829459	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	715	0.10145614398542437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.418967048747194E-4	0.0
10	0.0	1.418967048747194E-4	0.0	7.09483524373597E-4	0.0
11	0.0	1.418967048747194E-4	0.0	0.0026960373926196686	0.0
12	0.0	1.418967048747194E-4	0.0	0.005675868194988776	0.0
13	0.0	1.418967048747194E-4	0.0	0.0062434550144876534	0.0
14	0.0	1.418967048747194E-4	0.0	0.008513802292483164	0.0
15	0.0	1.418967048747194E-4	0.0	0.012061219914351148	0.0
16	0.0	1.418967048747194E-4	0.0	0.021000712321458472	0.0
17	0.0	1.418967048747194E-4	0.0	0.029230721204192196	0.0
18	0.0	1.418967048747194E-4	0.0	0.03320382894068434	1.418967048747194E-4
19	0.0	1.418967048747194E-4	0.0	0.038595903725923676	1.418967048747194E-4
20	0.0	1.418967048747194E-4	0.0	0.04512315215016077	1.418967048747194E-4
21	0.0	1.418967048747194E-4	0.0	0.05434643796701753	1.418967048747194E-4
22	0.0	1.418967048747194E-4	0.0	0.07137404255198386	1.418967048747194E-4
23	0.0	1.418967048747194E-4	0.0	0.09464510215143784	1.418967048747194E-4
24	1.418967048747194E-4	1.418967048747194E-4	0.0	0.12827462120674635	1.418967048747194E-4
25	1.418967048747194E-4	1.418967048747194E-4	0.0	0.1396263575967239	1.418967048747194E-4
26	1.418967048747194E-4	1.418967048747194E-4	0.0	0.158782412754811	1.418967048747194E-4
27	1.418967048747194E-4	1.418967048747194E-4	0.0	0.19709452307098524	1.418967048747194E-4
28	2.837934097494388E-4	1.418967048747194E-4	0.0	0.34722123682843836	1.418967048747194E-4
29	2.837934097494388E-4	1.418967048747194E-4	0.0	0.5661678524501303	1.418967048747194E-4
30	2.837934097494388E-4	1.418967048747194E-4	0.0	0.914808056327316	1.418967048747194E-4
31	2.837934097494388E-4	1.418967048747194E-4	0.0	1.6985035573503913	1.418967048747194E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	0.0054949187	29.599998	9
TCGTATG	25	0.0054949187	29.599998	10
AGGAGTA	60	1.3361805E-6	24.666668	5
GTATATC	40	0.0019304494	23.125002	1
CTAGGAC	40	0.0019304494	23.125002	3
TAGGAGT	100	5.329639E-10	22.199999	4
AGTCGGT	935	0.0	20.57754	11
CGAGCCG	190	0.0	20.447369	15
TTAAGAC	110	1.7516868E-9	20.181818	19
ATTGACT	165	0.0	20.181818	31
CAGTCGG	955	0.0	20.146599	10
GCAGTCG	965	0.0	20.129532	9
TCGGTGA	990	0.0	19.621212	13
GGCAGTC	1005	0.0	19.512438	8
GGGCAGT	1060	0.0	19.372643	7
TATTGAC	155	1.8189894E-12	19.096775	30
CGGTGAT	1010	0.0	19.049505	14
GTCGGTG	1010	0.0	19.049505	12
AAGACGG	215	0.0	18.930233	5
TGGGCAG	1090	0.0	18.839449	6
>>END_MODULE